박하의 in vitro 항산화활성
In vitro Antioxidant Activity of Mentha viridis L. and Mentha piperita L.
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새로운 기능성 소재를 발굴하기 위한 목적으로 2종의 박하(M. viridis (spicata), M. piperita)를 대상으로 수행된 몇 가지 in vitro 항산화계에서의 활성, 총페놀 함량 및 수율을 분석한 결과는 다음과 같다. 1. Superoxide 라디칼에 대한 박하의 소거능은 M. viridis가 M. piperita 보다 우수하였으며 특히 물 추출물은 대조물질인 ascorbic acid보다 우수하였다. 2. DPPH 라디칼에 대한 소거능은 메탄올 추출물 및 물 추출물 모두에서 M. viridis가 M. piperita보다 우수하였으며 특히 M. viridis의 메탄올 추출물은 롤α-tocopherol과 비슷한 수 준이었다. 3. 사람의 저밀도지단백질의 산화에 대한 박하의 저해활성을 분석한 결과는 M. viridis가 M. piperita 보다 우수하였으며 M. viridis의 모든 추출물 (65.88%~77.59%, 37.69%~87.21%)은 α-tocopherol (28.37%~66.54%)보다 월등하게 우수하였다. 4. Linoleic acid의 산화에 대한 저해효과는 M. viridis가 M. piperita보다 높았으며 α-tocopherol의 활성에 근접하는 수준이었다. 5. 총페놀 함량은 M. viridis (17.95% & 10.18%)가 M.piperita (15.44% & 9.19%)보다 높은 값을 나타내었으며 메탄올 추출물이 물 추출물보다 그 함량이 높았다. 6. 추출물의 수율은 메탄올 추출물 및 물 추출물 모두에서 M. piperita (14.1% & 14.6%)가 M. viridis (13.3% & 13.5%)보다 높게 나타나 항산화활성 및 총페놀 함량과는 상반되는 결과를 나타내었다. 이상의 실험결과를 종합할 때, 실험된 박하 중에서 M.viridis가 M. piperita보다 우수한 산업화 후보 자원이라고 사료된다.
Abstract
For selecting a new candidate as functional material, this study was conducted on in vitro antioxidant activityand total phenol content of methanol and water extracts prepared from two Mentha species ( L..(M. spicata L.) andMentha piperita L, Extracts of M. viridis showed more eficient scavenging activity on superoxide and DPPH.(α,α-diphenyl-β-picrylhydrazyl) radical and inhibitory activity on oxidation of human low density lipoprotein (LDL) induced by CuSO4and auto-oxidation of linoleic acid than those of M. piprita. Methanol extract (65.88%~77.59%) and water extract(37.69%~87.21%) of M. viridis also exhibited more potent inhibitory activity on LDL oxidation than that of α-tocopherol(28.37~66.54%) at 1~100. /. of final concentration. The total phenol contents of methanol extract and water extract of M.viridis (17.95% & 10.18%, respectively) as tannic acid equivalent were higher than those of M. piperita But the yields of methanol and water extracts of M. viridis (13.3% & 13.5%) were lower than those of M. piperita (14.1% &14.6%). The results implies that the extracts from M. viridis (spicata) is more useful material for industrialization as func-tional food than those from M. piperita.
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