초고압 추출 공정을 통한 고로쇠 목부 추출물의 항암활성 증진
Anticancer Activity of Acer mono Wood Extracted by Ultra High Pressure Extraction Process
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초록
We investigated a method to improve anticancer activities of Acer mono wood extracts by ultra high pressure extraction process. The A. mono was extracted by water at 40℃ and 300 MPa for 15 min (High Pressure Extraction, HPE). The extraction yield by ultra high pressure extraction process was 5.42%. The cytotoxicity on human normal lung cell (HEL299) of the extracts from HPE showed 21.54% lower than that from conventional water extraction at 100℃ in adding the maximum concentration of 1.0 ㎎/㎖. Ultra high pressure extracts process for 15 minutes extracts (HPE15) showed more potent scavenging effect than the control, BHA. On SOD-like test, the HPE15 showed highest activity as 32.4% at 1.0 ㎎/㎖ concentration. Human stomach adenocarcinoma, liver adenocarcinoma, breast adenocarcinoma and lung adenocarcinoma cell growth were inhibited up to about 67~79%, in adding 1.0 ㎎/㎖ of extracts from HPE. HPE was 20~25% higher than conventional water extraction. It was interesting that, among several cancer cell lines (stomach adenocarcinoma, liver adenocarcinoma), the growth of digestive related cancer cells were most effectively inhibited as about 75~79%. On in vivo experiment using ICR mice, the variation of body weight of mice group treated A. mono wood extracts from HPE of 100 ㎎/㎏/day concentration was very lower than control and other group. The survival times of group treated this extracts was 61.96% longer than that of the control group and this extracts showed the lower tumor weight, which were 10.49 g than positive control as 16.17 g. Based on these results, we could tell that the HPE wood extracts of A. mono had higher anticancer activity than conventional water extraction. The results of HPE showed obvious advantages in higher efficiency, shorter extraction time, at lower energy costs.
Abstract
We investigated a method to improve anticancer activities of Acer mono wood extracts by ultra high pressure extraction process. The A. mono was extracted by water at 40℃ and 300 MPa for 15 min (High Pressure Extraction, HPE). The extraction yield by ultra high pressure extraction process was 5.42%. The cytotoxicity on human normal lung cell (HEL299) of the extracts from HPE showed 21.54% lower than that from conventional water extraction at 100℃ in adding the maximum concentration of 1.0 ㎎/㎖. Ultra high pressure extracts process for 15 minutes extracts (HPE15) showed more potent scavenging effect than the control, BHA. On SOD-like test, the HPE15 showed highest activity as 32.4% at 1.0 ㎎/㎖ concentration. Human stomach adenocarcinoma, liver adenocarcinoma, breast adenocarcinoma and lung adenocarcinoma cell growth were inhibited up to about 67~79%, in adding 1.0 ㎎/㎖ of extracts from HPE. HPE was 20~25% higher than conventional water extraction. It was interesting that, among several cancer cell lines (stomach adenocarcinoma, liver adenocarcinoma), the growth of digestive related cancer cells were most effectively inhibited as about 75~79%. On in vivo experiment using ICR mice, the variation of body weight of mice group treated A. mono wood extracts from HPE of 100 ㎎/㎏/day concentration was very lower than control and other group. The survival times of group treated this extracts was 61.96% longer than that of the control group and this extracts showed the lower tumor weight, which were 10.49 g than positive control as 16.17 g. Based on these results, we could tell that the HPE wood extracts of A. mono had higher anticancer activity than conventional water extraction. The results of HPE showed obvious advantages in higher efficiency, shorter extraction time, at lower energy costs.
참고문헌 (34)
- Ahn WY, "Color index and coloring substances in Korean sugar maple,Acer mono Max.syrub", Journal of Korean Forest Society, 26, pp.7~12 (1975)
- Ames BN, "Oxidative DNA damage,cancer and aging.Oxygen and human disease", Annals of Internal Medicine, 107, pp.536~539 (1987)
- 이승철, "비단풀 물 추출물의 항산화력 및 항암활성", 한국식품영양과학회지, 35(10), pp.1304~1308 (2006), 한국식품영양과학회
- Barene AL, "Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluen", Journal of the American Oil Chemist's Society, 52, pp.59~63 (1975)
- Bennett PB, "High pressure biology and medicine", pp.1~428 (1998), University of Rochester Press
- Choi JH, "Studies on the anti-aging of Korean Ginseng", Journal of Food Science and Nutrition, 17, pp.506~515 (1985)
- Cross CE, "Oxygen radicals and human disease", Annals of Internal Medicine, 107, pp.526~545 (1987)
- Dool R, "The cause of cancer:Quantitative estimates of avoidable risks of cancer in the United States today", Food and Chemical Toxicology, 21, pp.512~513 (1983)
- Gutteridge JMC, "Antioxidant in nutrition, health, and disease", pp.1~62 (1994), Oxford University Press
- 정명훈, "고로쇠 수피 초고압 추출물의 항암활성 증진", 한국식품영양과학회지, 38(9), pp.1243~1252 (2009), 한국식품영양과학회
- 김영, "고로쇠와 우산고로쇠 나무의 항산화능 및 glutathione S-transferase 비교", 한국약용작물학회지, 16(6), pp.427~433 (2008), 한국약용작물학회
- 정성제, "약용 식물 추출물의 항산화 활성 검색", Journal of the Korean Society for Applied Biological Chemistry, 47(1), pp.135~140 (2004), 한국응용생명화학회
- Kang TB, "Studies on the inhibitory the growth of HL-60 leukemia cells", Biochemical Pharmacology, 54, pp.1013~1018 (1997)
- 이현용, "추출 공정 다변화를 통한 홍경천의 항암활성 증진효과", 한국약용작물학회지, 14(6), pp.317~321 (2006), 한국약용작물학회
- 김철희, "초고압 공정에 의한 홍경천의 독성 감소 및 항암활성 증진", 한국약용작물학회지, 15(6), pp.411~416 (2007), 한국약용작물학회
- Kim CM, "A study on the ingredients in the sap of Acer mono Max.and Betula costata T.in Mt.Jiri area", Korean Journal of Food Science and Technology, 20, pp.479~482 (1991)
- 김홍태, "인진쑥 methanol 추출물의 투여가 암이 유발된 마우스에서 보인 혈액생화학적 및 종양 무게에 미치는 영향", 한국임상수의학회지, 24(3), pp.372~378 (2007), 한국임상수의학회
- 권민철, "초고압 공정에 의한 복분자의 면역조절효능 비교", 한국약용작물학회지, 15(6), pp.398~404 (2007), 한국약용작물학회
- Kwon YS, "Activity of antioxidative compounds from the stem of Acer mono Max", Korean Journal of Medicinal Crop Science, 5, pp.302~306 (1997)
- 이현화, "흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교", 한국약용작물학회지, 16(2), pp.79~85 (2008), 한국약용작물학회
- 이세진, "감귤 농축액에서 배양한 운지버섯 배양추출물의 항산화 및 항암활성", 한국미생물·생명공학회지, 31(4), pp.362~367 (2003), 한국미생물·생명공학회
- 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회
- 김영, "초고압 저온처리에 의한 매자나무의 면역 활성", 한국약용작물학회지, 16(6), pp.439~445 (2008), 한국약용작물학회
- Marklund S, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", European Journal of Biochemistry, 47, pp.469~474 (1974)
- Min KJ, "Studies on the anticancer effect of Broussonetia kazinoki extracts", Korean Journal of Society and Food Science, 15, pp.231~237 (1999)
- Miquel J, "CRC Handbook of free radicals and antioxidants in biomedicine", pp.218~223 (1989), CRC press
- 문현식, "울릉도 자생 우산고로쇠나무의 수액채취와 주요성분", 한국약용작물학회지, 12(3), pp.249~254 (2004), 한국약용작물학회
- 이현용, "초음파 병행 추출이 마황과 복분자 열수 추출물의 면역활성 증진에 미치는 효과", KSBB Journal, 19(2), pp.113~117 (2004), 한국생물공학회
- Park JW, "New targeted agents for hepatocellular carcinoma", Journal of the Korean Liver Cancer Study Group, 8, pp.16~17 (2008)
- Park SY, "Screening and isolation of the antitumor agents from medicinal plants(I)", Korean Journal of Pharmacognosy, 23, pp.264~267 (1992)
- Syed Abdul Qadir, "고로쇠와 우산고로쇠 나무의 부위별 항암 및 면역조절능 비교", 한국약용작물학회지, 15(6), pp.405~410 (2007), 한국약용작물학회
- Terazawa M., "Phenolic compounds in living tissues of woods III.Platyphylloside,a new diarylheptanoid glucoside from the green back of shirakamba(Betula platyphylla Sukatchev var.japonica Hara)", Mokuzai Gakkaishi, 30, pp.391~403 (1984)
- Trush MA, "Activation of pharmacologic agents to radical inermediates.Implications for the role of free radicals in drug action and toxicity", Biochemical Pharmacology, 31, pp.3335~3346 (1982)
- Yoon SL, "Utilization and tapping of the sap from briches and maples", Mokchae Konghak, 20, pp.15~20 (1992)