지치의 생육시기 및 재배지역별 시코닌계 색소 함량 및 항산화 활성
Investigation of Shikonin Pigments and Antioxidant activity of the Roots from Lithospermum erythrorhizon according to the Different Growth Stages and Areas of Cultivation
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초록
This study was conducted to evaluate the quality variation of Lithospermum radix on the pigment contents and antioxidant activities according to different growth stages and areas of cultivation. Acetylshikonin contents showed the tendency to decrease gradually from the middle of July (0.28%) to the end of August (0.05%) and then the content was increased again to the end of October (0.25%). Shikonin content was detected as small amount of about 0.009% during the period. The weight of plants was increased from the end of September to the end of October and showed the highest value as 19.8 g on October 25. ROS scavenging activity was the highest in the early of October as IC_50 value of 0.11 ㎍/mL. Lithospermum radix of September showed lower ROS scavenging activities than those of other growth stages as IC_50 value of 1.02 and 0.49 ㎍/mL on September 9 and September 27, respectively. Among 17 areas cultivated Lithospermum radix, 10 areas (59%)showed 0.05-0.10% of acetylshikonin contents and 3 areas (18%) were measured to contain 0.16-0.26% of acetylshikonin.
Abstract
This study was conducted to evaluate the quality variation of Lithospermum radix on the pigment contents and antioxidant activities according to different growth stages and areas of cultivation. Acetylshikonin contents showed the tendency to decrease gradually from the middle of July (0.28%) to the end of August (0.05%) and then the content was increased again to the end of October (0.25%). Shikonin content was detected as small amount of about 0.009% during the period. The weight of plants was increased from the end of September to the end of October and showed the highest value as 19.8 g on October 25. ROS scavenging activity was the highest in the early of October as IC_50 value of 0.11 ㎍/mL. Lithospermum radix of September showed lower ROS scavenging activities than those of other growth stages as IC_50 value of 1.02 and 0.49 ㎍/mL on September 9 and September 27, respectively. Among 17 areas cultivated Lithospermum radix, 10 areas (59%)showed 0.05-0.10% of acetylshikonin contents and 3 areas (18%) were measured to contain 0.16-0.26% of acetylshikonin.
참고문헌 (29)
- 안영희, "지치(Lithospermum erythrorhizon) 개체군 자생지의 생태학적 특성", 생약학회지, 40(4), pp.289~297 (2009), 한국생약학회
- Beckman JS, "Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide", Proceedings of the National Academy of Sciences of the United States of America, 87, pp.1620~1624 (1990)
- Biesalski HK, "Free radical theory of aging", Current Opinion in Clinical Nutrition and Metabolic Care, 5, pp.5~10 (2002)
- Chang YS, "Inhibition of platelet aggregation by shikonin derivatives isolated from Arnebia euchromoma", Planta Medica, 59, pp.401~404 (1993)
- Han J, "Antioxidants from a Chinese medicinal herb-Lithospermum erythrorhizon", Food Chemistry, 106, pp.2~10 (2008)
- Hisamichi SJ, "Structures of new minor pigments and isolation of two isomers of shikonin derivatives from Lithospermum erythrorhizon Sieb. et Zucc", Shoyakugaku Zasshi, 36, pp.1541~1559 (1982)
- Hwang SY, "Isolation and quantitative analysis of acetylshikonin from Lithospermi Radix", Korean Journal of Pharmacognosy, 31, pp.295~299 (2000)
- 장주리, "도라지 부탄올 추출물의 항산화 및 nitric oxide 생성 저해 효과", 한국식품저장유통학회지, 18(1), pp.65~71 (2011), 한국식품저장유통학회
- 정향숙, "복분자 추출물의 나노입자화를 통한 항산화 및 미백 효과 증진", 한국약용작물학회지, 17(2), pp.81~89 (2009), 한국약용작물학회
- HyeSookKang, "Scavenging Effect of Korean Medicinal Plants on the Peroxynitrite and Total ROS", Natural Product Sciences, 9(2), pp.73~79 (2003), 한국생약학회
- 김금숙, "지치뿌리로부터 분리한 Acetylshikonin의 LDL 산화 저해활성과 FPTase 저해활성", Journal of Applied Biological Chemistry, 52(4), pp.221~225 (2009), 한국응용생명화학회
- Lebel CP, "Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress", Chemical Research in Toxicology, 5, pp.227~231 (1992)
- 이보한, "간경변증 환자에서 말초혈액 Nitric Oxide 활성도 측정의 의의", 대한내과학회지, 73(3), pp.251~257 (2007), 대한내과학회
- Lee HJ, "Shikonin, Acetylshikonin, and Isobutyroylshikonin Inhibit VEGF-induced Angiogenesis and Suppress Tumor Growth in Lewis Lung Carcinoma-bearing Mice", YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 128, pp.1681~1688 (2008), The pharmaceutical society of Japan
- Lee JW, "Determination of total phenolic compounds from the fruit of Rubus coreanum and antioxidative activity", Journal of the Korean Society of Food Science and Nutrition, 29, pp.943~ (2000)
- Lee KS, "The encyclopedia of oriental herbal medicine", pp.4657~4663 (1998), Jungdam Pubishing Co
- Tiana L, "Evaluation of the anti-neuraminidase activity of the traditional Chinese medicines and determination of the anti-influenza a virus effects of the neuraminidase inhibitory TCMs in vitro and in vivo", Journal of Ethnopharmacology, 137, pp.534~542 (2011)
- 오혜인, "울금과 강황의 항산화 및 항염증 활성 비교연구", 대한본초학회지, 25(1), pp.83~91 (2010), 대한본초학회
- 오영지, "천궁(Cnidium officinale Makino) 지상부 추출물의 항산화 활성 평가", 한국약용작물학회지, 18(6), pp.373~378 (2010), 한국약용작물학회
- Ordoudi SA, "Structure-radical scavenging activity relationship of alkannin", shikonin derivatives, 2011, pp.124, 171~176
- Jun Yeon Park, "Phytochemical Constituents from the Seeds of Lithospermum erythrorhizon", Natural Product Sciences, 15(4), pp.181~184 (2009), 한국생약학회
- Jun Yeon Park, "A Paraben Derivative from the Seeds of Lithospermum erythrorhizon", Journal of the Korean Society for Applied Biological Chemistry, 52(6), pp.643~645 (2009), 한국응용생명화학회
- Sekine T, "Evaluation of superoxide anion radical scavenging activity of shikonin by electron spin resonance", International Journal of Pharmaceutics, 174, pp.133~139 (1998)
- Staniforth V, "Shikonins, phytocompounds from Lithospermum erythrorhizon, inhibit the transcriptional activation of human TNF-α promoter in vivo", The Journal of Biological Chemistry, 279, pp.5877~5885 (2004)
- Tabata M, "Antimicrobial activity of quinone derivatives from Echiumlycopsis callus cultures", Planta Medica, 44, pp.234~236 (1982)
- Tanaka S, "A comparative study on anti-inflammatory activities of the enantiomers, shikonin and alkannin", Journal of Natural Products, 49, pp.466~469 (1986)
- Weng XC, "Antioxidant properties of components extracted from puccoon (Lithospermum erythrorhizon Sieb. et Zucc.)", Food Chemistry, 69, pp.143~146 (2000)
- Yu BP, "Aging and oxidative stress: modulation by dietary restriction", Free Radical Biology & Medicine, 21, pp.651~668 (1996)
- Zhang J, "Nitric oxide activation of poly(ADP-ribose) synthetase in neurotoxicity", Science, 263, pp.687~689 (1994)