인지능 개선 효과 증진을 위한 백삼 추출물 조제 연구
Study on White Ginseng Extract Preparation for Cognition Improvement
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초록
Background: The study was conducted to elucidate the extraction conditions under which white ginseng has cognition-improving efficacy. Methods and Results: Extracts from white ginseng under different solvent and temperature conditions were analyzed for ginsenoside content and inhibitory effect on N-methyl-D-aspartate (NMDA) receptor and acetylcholinesterase. The total ginsenoside contents and amounts of ginsenoside Rb1 plus ginsenoside Rg1 from the 1st extracts (prepared with EtOH/H2O as solvent) were higher than those from the 2nd extracts (extracted with H2O after the 1st EtOH/H2O extraction). The contents in the 1st and 2nd extracts produced at 80℃ were also higher than those obtained at 50℃. Samples from the 1st extraction at 80℃ indicated higher inhibitory activities on NMDA receptors-whose excessive activation is thought to mediate the calcium-dependent neurotoxicity associated with several neurodegenerative diseases-than those from the 2nd extraction. Among the samples prepared at varying temperatures, the extract prepared at 50℃ showed the highest suppression activity on NMDA receptors. Note, however, that the extracts from the 2nd extraction at 50℃ inhibited acetylcholinesterase-whose inhibition could be a therapeutic strategy for neurodegenerative diseases with cognitive deficits and memory malfunction-more effectively than those from the 1st extraction. Conclusions: To enhance the cognition-improving activity of white ginseng extract, it is suggested that the extracts be utilized after being combined the 1st extracts (made with EtOH/H2O solvent) and the 2nd extracts (prepared with H2O) at low temperature.
Abstract
Background: The study was conducted to elucidate the extraction conditions under which white ginseng has cognition-improving efficacy. Methods and Results: Extracts from white ginseng under different solvent and temperature conditions were analyzed for ginsenoside content and inhibitory effect on N-methyl-D-aspartate (NMDA) receptor and acetylcholinesterase. The total ginsenoside contents and amounts of ginsenoside Rb1 plus ginsenoside Rg1 from the 1st extracts (prepared with EtOH/H2O as solvent) were higher than those from the 2nd extracts (extracted with H2O after the 1st EtOH/H2O extraction). The contents in the 1st and 2nd extracts produced at 80℃ were also higher than those obtained at 50℃. Samples from the 1st extraction at 80℃ indicated higher inhibitory activities on NMDA receptors-whose excessive activation is thought to mediate the calcium-dependent neurotoxicity associated with several neurodegenerative diseases-than those from the 2nd extraction. Among the samples prepared at varying temperatures, the extract prepared at 50℃ showed the highest suppression activity on NMDA receptors. Note, however, that the extracts from the 2nd extraction at 50℃ inhibited acetylcholinesterase-whose inhibition could be a therapeutic strategy for neurodegenerative diseases with cognitive deficits and memory malfunction-more effectively than those from the 1st extraction. Conclusions: To enhance the cognition-improving activity of white ginseng extract, it is suggested that the extracts be utilized after being combined the 1st extracts (made with EtOH/H2O solvent) and the 2nd extracts (prepared with H2O) at low temperature.
참고문헌 (26)
- Chen HSV, "Open-channel block of N-methyl-Daspartate(NMDA)responses by memantine : Therapeutic advantage against NMDA receptor-mediated neurotoxicity", Journal of Neuroscience, 12, pp.4427~4436 (1992)
- 최장숙, "백삼의 페놀산 조성과 항산화 활성", Journal of Ginseng Research, 30(1), pp.22~30 (2006), 고려인삼학회
- Giacobini E., "Cholinergic function and Alzheimer’s disease", International Journal of Geriatric Psychiatry, 18, pp.S1~S5 (2003)
- Greig NH, "Selective butyrylcholinesterase inhibition elevates brain acetylcholine, augments learning and lowers Alzheimer β-amyloid peptide in rodent", Proceedings of the National Academy of Sciences of the United States of America, 102, pp.17213~17218 (2005)
- 현미선, "RAW 264.7 세포에서 LPS에 의해 유도된 염증에 대한 백삼, 홍삼, 발효홍삼의 항염효과에 대한 비교 연구", Journal of Applied Biological Chemistry, 52(1), pp.21~27 (2009), 한국응용생명화학회
- 전보현, "고지방식으로 생육한 생쥐 간에서 백삼과 홍삼 추출물의 항산화 효과", Journal of Ginseng Research, 29(3), pp.138~144 (2005), 고려인삼학회
- Jung NP, "Cytotoxicity of white and red ginseng against cancer cells and their effects on the cell cycle", Journal of Ginseng Research, 24, pp.183~187 (2000)
- Kennedy DO, "Modulation of cognition and mood following administration of single doses of Ginkgo biloba, ginseng, and a ginkgo", ginseng combination to healthy young adults, 2002, pp.75, 739~751
- 김동휘, "고려인삼의 식품 안전성에 관한 임상 연구", 한국약용작물학회지, 23(3), pp.185~189 (2015), 한국약용작물학회[DOI]
- Kim S, "Inhibitory effect of ginsenosides on NMDA receptor-mediated signals in rat hippocampal neurons", Biochemical and Biophysical Research Communications, 296, pp.247~254 (2002)
- 김세미, "LPS에 의해 활성화된 RAW264.7 대식세포에서 수삼깍두기의 항염증 효과", 한국식생활문화학회지, 30(2), pp.197~205 (2015), 한국식생활문화학회[DOI]
- Kim US, "Study of the effects of different products of ginseng radix aqua-acupuncture on the alloxan-induced diabetic rats", The Acupuncture, 6, pp.1~13 (1989)
- Kim YC, "Ginsenoside Rb1 and Rg3 protect cultured rat cortical cells from glutamate-induced neurodegeneration", Journnal of Neuroscience Research, 53, pp.426~432 (1998)
- Young Ock Kim, "Effects of Ginseng Radix on the ischemia-induced 4-vessel occlusion and cognitive impairments in the rat", Journal of Ginseng Research, 31(1), pp.44~50 (2007), 고려인삼학회
- Lee E, "20(S)-ginsenoside Rh2, a newly identified active ingredient of ginseng, inhibits NMDA receptors in cultured rat hippocampal neurons", European Journal of Pharmacology, 536, pp.69~77 (2006)
- 이미라, "백삼과 홍삼추출물의 학습수행과 Acetylcholinesterase 억제에 미치는 효과", Journal of Ginseng Research, 32(4), pp.341~346 (2008), 고려인삼학회
- 이승은, "Trimethyltin으로 유도된 기억장애 흰쥐에서 백삼의 신경보호효과", 한국약용작물학회지, 19(6), pp.456~463 (2011), 한국약용작물학회
- Lim JH, "Protection of ischemic hippocampal neurons by ginsenoside Rb1, a main ingredient of ginseng root", Neuroscience Research, 28, pp.191~200 (1997)
- Lopes JP, "Galantamine potentiates the neuroprotective effect of memantine against NMDA-induced excitotoxicity", Brain and Behavior, 3, pp.67~74 (2013)
- Mook-Jung I, "Ginsenoside Rb1and Rg1 improve spartial learning and increase hippocampal synaptophysin level in mice", Journal of Neuroscience Research, 63, pp.509~515 (2001)
- Perry EK, "Changes in brain cholinesterases in senile dementia of Alzheimer type", Neuropathology and Applied Neurobiology, 4, pp.273~277 (1978)
- Radad K, "Ginsenosides Rb1 and Rg1 effects on mesencephalic dopaminergic cells stressed with glutamate", Brain Research, 1021, pp.41~53 (2004)
- Reay JL, "Panax ginseng(G115)improves aspects of working memory performance and subjective ratings of calmness in healthy young adults", Human Psychopharmacology : Clinical and Experimental, 25, pp.462~471 (2010)
- 성금수, "Acetaminophen 유도 간독성에 대한 백삼과 홍삼 추출물의 간보호 효과", Journal of Ginseng Research, 29(3), pp.131~137 (2005), 고려인삼학회
- Shin JY, "The differnce of ginsenoside compositions according to the conditions of extraction and fractionation of crude ginseng saponins", Korean Journal of Food Science and Technology, 33, pp.282~287 (2001)
- 성정훈, "고려인삼에 의한 신경면역 및 염증반응 조절 : 백삼사포닌에 의한 교세포에서의 TNF-α, IL-1β 및 NO 생성 증가", Journal of Ginseng Research, 28(2), pp.120~126 (2004), 고려인삼학회