본문 바로가기
KERD
논문

국내 대추 품종의 구분을 위한 InDel 마커의 개발

Development of Insertion or Deletion Markers to Distinguish Korean Jujube Cultivars

이경희|이미선|조남수|박상익|김주혁|이이|김호방|이문순|길진수|김문교|Enkhtsetseg Yeruult|오하경
한국약용작물학회|2021.08|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제29권 제4호|pp. 282-292|DOI 10.7783/KJMCS.2021.29.4.282

0

조회

0

다운로드

4

인용

0

참고문헌

키워드

CultivarMolecular MarkerInsertion or DeletionZiziphus jujubaNext-generation Sequencing

초록

Background: Jujube (Ziziphus jujuba Mill.) friuts are rich in nutrients and an economically and ecologically important medicinal plant in Korea. However, as it is difficult to distinguish jujube cultivars using morphological characters, the development of molecular markers is necessary to protect and distinguish Korean jujube cultivars. Methods and Results: Next-generation sequencing analysis was performed on six major Korean cultivars; Bokjo, Boeun, Chuseok, Mudeung, Geumseong, and Wolchul. Sites with an insertion or deletion (InDel) were identified by comparison from the sequence information of the six Korean cultivars using the CLC Genomics Workbench. Among the identified InDels, we developed ZjInDel-1 and Zj-InDel-2 markers, which could distinguish Bokjo, Chuseok, and Boeun from the other Korean cultivars. Conclusions: The InDel markers developed in this study could be used for classification of domestic jujube cultivars and protection of the elite ones.

Abstract

Background: Jujube (Ziziphus jujuba Mill.) friuts are rich in nutrients and an economically and ecologically important medicinal plant in Korea. However, as it is difficult to distinguish jujube cultivars using morphological characters, the development of molecular markers is necessary to protect and distinguish Korean jujube cultivars. Methods and Results: Next-generation sequencing analysis was performed on six major Korean cultivars; Bokjo, Boeun, Chuseok, Mudeung, Geumseong, and Wolchul. Sites with an insertion or deletion (InDel) were identified by comparison from the sequence information of the six Korean cultivars using the CLC Genomics Workbench. Among the identified InDels, we developed ZjInDel-1 and Zj-InDel-2 markers, which could distinguish Bokjo, Chuseok, and Boeun from the other Korean cultivars. Conclusions: The InDel markers developed in this study could be used for classification of domestic jujube cultivars and protection of the elite ones.

참고문헌 (44)

  1. 방미희, "국산 건대추의 외형 등급별 품질특성 연구", 한국약용작물학회지, 28(6), pp.455~462 (2020), 한국약용작물학회
  2. Chakravarthi BK, "SSR marker based DNA fingerprinting and diversity study in rice(Oryza sativa L)", African Journal of Biotechnology, 5, pp.684~688 (2006)
  3. Chen R, "Development of InDel markers for Brassica rapa based on a high-resolution melting curve", Horticultural Plant Journal, 7, pp.31~37 (2021)
  4. Chiou CY, "The genetic relationships of Indian jujube(Ziziphus mauritiana Lam.) cultivars using SSR markers", Heliyon, 6, pp.e05078~ (2020)[DOI]
  5. Choi KJ, "International union for the protection of new varieties of plants(UPOV)and its 1991 convention", Korean Journal of Horticultural Science and Technology, 20, pp.151~159 (2002)
  6. Cyong JC, "Cyclic adenosine monophosphate in fruits of Zizyphus jujuba", Phytochemistry, 19, pp.2747~2748 (1980)
  7. Cyong JC, "Identification of guanosine 3′ : 5′-monophosphate in the fruit of Zizyphus jujuba", Phytochemistry, 21, pp.1871~1874 (1982)
  8. Doyle JJ, "Isolation of plant DNA from fresh tissue", Focus, 12, pp.39~40 (1990)
  9. Evreinoff VA, "Notes sur le Jujubier(Zizyphus sativa G.)", Journal D'agriculture Traditionnelle et de Botanique Appliquée, 11, pp.177~187 (1964)
  10. Gao QH, "The jujube(Ziziphus jujuba Mill.) fruit : A review of current knowledge of fruit composition and health benefits", Journal of Agricultural and Food Chemistry, 61, pp.3351~3363 (2013)
  11. Han BH, "Sedative activity and its active components of Zizyphi Fructus", Archives of Pharmacal Research, 10, pp.208~211 (1987)
  12. Hayashi K, "Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes", Theoretical and Applied Genetics, 113, pp.251~260 (2006)
  13. Jain A, "InDel markers: An extended marker resource for molecular breeding in chickpea", PLoS One, 14, pp.e0213999~ (2019)[DOI]
  14. Kim KH, "Comprehensive survey of genetic diversity in chloroplast genomes and 45S nrDNAs within Panax ginseng species", PLoS One, 10, pp.e0117159~ (2015)[DOI]
  15. Kumar S, "MEGA7 : Molecular evolutionary genetics analysis version 7. 0 for bigger datasets", Molecular Biology and Evolution, 33, pp.1870~1874 (2016)
  16. Li JW, "Nutritional composition of five cultivars of Chinese jujube", Food Chemistry, 103, pp.454~460 (2007)
  17. Li S, "Genetic diversity and population structure of Chinese jujube(Ziziphus jujuba Mill.) and sour jujube(Ziziphus acidojujuba Mill.) using inter-simple sequence repeat(ISSR)Markers", Molecular Plant Breeding, 12, pp.1~21 (2021)
  18. Li S, "Development and application of cucumber InDel markers based on genome re-sequencing", Journal of Plant Genetic Resources, 14, pp.278~283 (2013)
  19. Li W, "An InDel-based linkage map of hot pepper(Capsicum annuum)", Molecular Breeding, 35, pp.1~10 (2015)
  20. Liu B, "Development of InDel markers for Brassica rapa based on whole-genome re-sequencing", Theoretical and Applied Genetics, 126, pp.231~239 (2013)
  21. Liu J, "A Chinese jujube(Ziziphus jujuba Mill. )fruit-expressed sequence tag(EST) library : Annotation and EST-SSR characterization", Scientia Horticulturae, 165, pp.99~105 (2014)
  22. Liu K, "PowerMarker : An integrated analysis environment for genetic marker analysis", Bioinformatics, 21, pp.2128~2129 (2005)
  23. Liu M, "RAPD analysis on the cultivars, strains and related species of Chinese jujube", Acta Horticulturae, 622, pp.477~484 (2002)
  24. Liu MJ, "A taxonomic study on the genus Ziziphus", Acta Horticulturae, 390, pp.161~166 (1994)
  25. Ma QH, "Development and characterization of SSR markers in Chinese jujube(Ziziphus jujuba Mill.) and its related species", Scientia Horticulturae, 129, pp.597~602 (2011)
  26. Moghaddam SM, "Developing market class specific InDel markers from next generation sequence data in Phaseolus vulgaris L", Frontiers in Plant Science, 5, pp.185~ (2014)[DOI]
  27. Na HS, "Effect of jujube methanol extract on the hepatotoxicity in CCl4-treated rats", Journal of the Korean Society of Food Science and Nutrition, 25, pp.839~845 (1996)
  28. 남재익, "I-SSR 표지자분석을 이용한 대추나무 품종간 유연관계 분석", 한국산림과학회지, 102(1), pp.59~65 (2013), 한국산림과학회
  29. Okamura N, "Studies on the constituents of Zizyphi Fructus. III. Structures of dammarane-type saponins", Chemical and Pharmaceutical Bulletin, 29, pp.676~683 (1981)
  30. Outlaw WH Jr, "The jujube(Ziziphus jujuba Mill.), a multipurpose plant", Economic Botany, 56, pp.198~200 (2002)
  31. 박기찬, "차세대염기서열분석법을 이용한 잔대의 SSR 마커 개발", 한국약용작물학회지, 25(6), pp.411~417 (2017), 한국약용작물학회[DOI]
  32. 박상익, "엽록체기반 SSR marker를 이용한 당귀의 기원 판별", 한국약용작물학회지, 25(6), pp.361~366 (2017), 한국약용작물학회[DOI]
  33. 박영기, "대추(Zyziphus jujuba) 품종별 성숙정도에 따른 항산화 활성, 총페놀 함량, 비타민 C 함량 및 당함량", 한국자원식물학회지, 29(5), pp.539~546 (2016), 한국자원식물학회[DOI]
  34. Qi J, "Establishment of ISSR-PCR reaction system in Chinese jujube", Acta Agriculturae Boreali-Sinica, 23, pp.209~212 (2008)
  35. Qu ZZ, "China fruit’s monograph—Chinese jujube volume", pp.229~ (1993), China Forestry Publishing House
  36. Rhee YK, "Inhibitory effect of Zizyphi Fructus on β-glucuronidase and tryptophanase of human intestinal bacteria", Korean Journal of Food Science and Technology, 30, pp.199~205 (1998)
  37. Shahhoseini R, "A study on genetic variation in Iranian jujube(Zizyphus jujuba Mill. )genotypes using molecular AFLP marker", Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 20, pp.55~68 (2012)
  38. Smith JSC, "Fingerprinting crop varieties", Advances in Agronomy, 47, pp.85~140 (1992)
  39. Wang S, "Isolation and characterization of microsatellite markers and analysis of genetic diversity in Chinese jujube(Ziziphus jujuba Mill.)", PLoS One, 9, pp.e99842~ (2014)[DOI]
  40. Yagi A, "Studies on the constituents of Zizyphi Fructus. IV. Isolation of an anti-allergic component, ethyl alpha-D-fructofuranoside from EtOH extract of Zizyphi Fructus(author's transl)", Yakugaku zasshi: Journal of the Pharmaceutical Society of Japan, 101, pp.700~707 (1981)
  41. Yagi A, "Studies on the constituents of Zizyphi Fructus. I. structure of three new p-coumaroylates of alphitolic acid", Chemical and Pharmaceutical Bulletin, 26, pp.1798~1802 (1978)
  42. Yang J, "In silico identification and experimental validation of Insertion-Deletion polymorphisms in tomato genome", DNA research, 21, pp.429~438 (2014)
  43. Zhao J, "Rapid SNP discovery and a RAD-based high-density linkage map in jujube(Ziziphus Mill.)", PLoS One, 9, pp.e109850~ (2014)[DOI]
  44. Zhao Z, "Characterization of water soluble polysaccharides from organs of Chinese jujube(Ziziphus jujuba Mill. cv. Dongzao)", European Food Research and Technology, 226, pp.985~989 (2008)

관련 논문AI추천

매실나무와 살구나무, 종간잡종의 구별을 위한 InDel 마커의 개발이이, 김문교, 김주혁, 이동욱, 이미선, 조남수한국약용작물학회 · 한국약용작물학회지2022 · 제30권 제3호국내 작약 품종을 저비용으로 식별하기 위한 InDel 마커 개발이이, 허목, 구성철, 김리경, 김문교, 김이현, 김혜원, 박우태, 박재완, 안우석, 오태영, 이미선, 이정훈, 장수연, 정진태한국약용작물학회 · 한국약용작물학회지2025 · 제33권 제5호오미자과 식물 종 판별을 위한 엽록체 기반 InDel 마커의 개발이이, 강상호, 권수진, 길진수, 김창국, 김효진, 이재복, 이정호, 정희정, 홍창표한국약용작물학회 · 한국약용작물학회지2021 · 제29권 제1호닥나무 속 식물의 엽록체 유전체 기반 InDel 마커의 개발이이, 김윤아, 김정성, 김주혁, 신창섭, 이미선, 이은지, 최용규한국자원식물학회 · 한국자원식물학회지2023 · 제36권 제4호일천궁과 토천궁의 구별을 위한 InDel 마커의 개발이이, 김동휘, 김호방, 문성진, 박민선, 박재완, 손동균, 이미선, 이정훈한국약용작물학회 · 한국약용작물학회지2025 · 제33권 제1호모란과 작약의 구별을 위한 엽록체 기반 InDel 마커의 개발정 희, 이이, 길진수, 김문교, 김세림, 박소현, 윤기훈, 이미선, 이재복, 정진태, 정희정한국약용작물학회 · 한국약용작물학회지2022 · 제30권 제6호

이 간행물의 다른 논문 (6건)

지황 생장의 파괴·비파괴적 해석남효훈, 김광섭, 서영진pp. 233-241감초 유전자원의 생육 및 수량 특성이정훈, 마경호, 한종원, 우선희, 정진태, 오명원, 윤영호, 손동균, 황호섭pp. 242-252키토산과 UV처리에 따른 2년생 인삼의 생육특성 및 성분변화장인배, 장인복, 문지원, 서수정, 김영창pp. 253-262db/db마우스에서 Aspergillus awamori로 발효한 황기 추출물의 항당뇨 및 지질개선 효능송빛나, 김영수, 이미연, 이성현, 박보람, 최지호, 박신영, 이은정, 최활, 윤원찬pp. 263-272황기 재배지에서 토양의 물리적 특성에 따른 습해 정도와 재배적지 기준 설정이은송, 현근수, 김용일, 안태진, 장재기pp. 273-281
원문 보기