매실나무와 살구나무, 종간잡종의 구별을 위한 InDel 마커의 개발
Development of InDel Markers to Distinguish between Prunus mume, Prunus armeniaca var. ansu, and their Interspecific Hybrids
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Background: Prunus mume Sieb. et. Zucc. is an important medicinal resource, and its fruit is used as a health food worldwide. However, this plant is often confused with Prunus armeniaca var. ansu., a related but distinct deciduous species. To distinguish between these plants, we developed co-dominant insertion-deletion (InDel) markers. Methods and Results: DNA was extracted from P. mume and P. armeniaca leaves and subjected to next-generation sequencing (NGS) analysis. After assembly, 30 polymorphic loci were identified. Primers were designed for the polymorphic loci and used to genotype P. mume and P. armeniaca. Using two InDel markers developed from the polymorphic loci, we distinguished between P. mume and P. armeniaca, and also identified the genetic hybrids between P. mume and P. armeniaca.
Abstract
Background: Prunus mume Sieb. et. Zucc. is an important medicinal resource, and its fruit is used as a health food worldwide. However, this plant is often confused with Prunus armeniaca var. ansu., a related but distinct deciduous species. To distinguish between these plants, we developed co-dominant insertion-deletion (InDel) markers. Methods and Results: DNA was extracted from P. mume and P. armeniaca leaves and subjected to next-generation sequencing (NGS) analysis. After assembly, 30 polymorphic loci were identified. Primers were designed for the polymorphic loci and used to genotype P. mume and P. armeniaca. Using two InDel markers developed from the polymorphic loci, we distinguished between P. mume and P. armeniaca, and also identified the genetic hybrids between P. mume and P. armeniaca.
참고문헌 (24)
- 최보경, "매실농축액의 에틸카바메이트 분석과 노출량 평가", 한국식품조리과학회지, 31(2), pp.112~117 (2015), 한국식품조리과학회
- 전재범, "변이밀집영역 유래 27개 InDel 마커를 이용한 콩(Glycine max (L.) Merrill) 신품종 판별 및 국내 149 품종과 유연관계 분석", 한국자원식물학회지, 32(5), pp.519~542 (2019), 한국자원식물학회[DOI]
- Corrado G, "SNP genotyping reveals genetic diversity between cultivated landraces and contemporary varieties of tomato", BMC Genomics, 14, pp.835~ (2013)[DOI]
- Daniell H, "Chloroplast genomes: Diversity, evolution, and applications in genetic engineering", Genome Biology, 17, pp.134~ (2016)[DOI]
- Gao ZH, "Microsatellite markers and genetic diversity in Japanese apricot(Prunus mume)", HortScience, 39, pp.1571~1574 (2004)
- 길진수, "SSR 마커를 이용한 산양삼의 유전적 다양성 분석", 한국약용작물학회지, 25(6), pp.389~396 (2017), 한국약용작물학회[DOI]
- 정대희, "Genome-Wide SSR 마커를 이용한 주요 산지별 참당귀의 유전다양성 분석", 한국약용작물학회지, 27(6), pp.376~382 (2019), 한국약용작물학회[DOI]
- 김진기, "절화장미 품종간 정역교배에 있어서 EST-SSR 마커의 유전", 원예과학기술지, 28(4), pp.618~626 (2010), 한국원예학회
- 김문교, "국내 대추 품종의 구분을 위한 InDel 마커의 개발", 한국약용작물학회지, 29(4), pp.282~292 (2021), 한국약용작물학회[DOI]
- Kwon YJ, "Volatile components of apricot(Prunus armeniaca var. ansu Max. )and Japanese apricot(Prunus mume S ieb. e t Zu c. )", Journal of Applied Biological Chemistry, 33, pp.319~324 (1990)
- 이정란, "DNA 염기서열에 기초한 벼과 잡초의 분자생물학적 동정", Weed & Turfgrass Science, 4(1), pp.26~34 (2015), 한국잔디학회[DOI]
- Lv Y, "mInDel: A high-throughput and efficient pipeline for genome-wide InDel marker development", BMC Genomics, 17, pp.290~ (2016)[DOI]
- 백일영, "매실 추출물 섭취가 에너지기질 및 피로물질 변화에 미치는 영향", 생명과학회지, 20(1), pp.49~54 (2010), 한국생명과학회
- Pan IC, "Complete chloroplast genome sequence of an orchid model plant candidate:Erycina pusilla apply in tropical Oncidium breeding", PloS One, 7, pp.34738~ (2022)[DOI]
- Park IK, "The complete chloroplast genomes of six Ipomoea species and indel marker development for the discrimination of authentic Pharbitidis Semen(Seeds of I. nil or I. purpurea)", Frontiers in Plant Science, 9, pp.965~ (2018)[DOI]
- Park IK, "Sequencing and comparative analysis of the chloroplast genome of Angelica polymorpha and the development of a novel indel marker for species identification.", Molecules, 24, pp.1038~ (2019)[DOI]
- 박상익, "엽록체기반 SSR marker를 이용한 당귀의 기원 판별", 한국약용작물학회지, 25(6), pp.361~366 (2017), 한국약용작물학회[DOI]
- Park YS, "Effect of Prunus mume extract on the sensory quality and shelf life of cooked rice", Korean Journal of Food and Cookery Science, 14, pp.503~508 (1998)
- Sim JH, "Screening of antioxidant in Fructus Mune(Prunus mune Sieb. Et Zucc. )extract", Journal of Applied Biological Chemistry, 45, pp.119~123 (2002)
- 손황배, "콩 전장 유전체 기반의 202개 InDel 마커를 활용한 한국 콩 품종의 염색체 재조합 양상 구명", 한국육종학회지, 49(3), pp.213~223 (2017), 한국육종학회[DOI]
- Tao R, "Characterization and cDNA cloning for Sf-RNase, a molecular marker for self-compatibility, in Japanese apricot(Prunus mume)", Journal of the Japanese Society for Horticultural Science, 71, pp.595~600 (2002)
- Tao R, "Molecular markers for self-compatibility in Japanese apricot(Prunus mume)", HortScience, 35, pp.1121~1123 (2000)
- Xue S, "Comparative analysis of the complete chloroplast genome among Prunus mume, P. armeniaca, and P. salicina", Horticulture Research, 6, pp.89~ (2019)[DOI]
- 유수정, "살구 추출물의 항산화성, 항돌연변이성 및 세포독성 효과", Food Science and Preservation, 14(2), pp.220~225 (2007), 한국식품저장유통학회