Lipopolysaccharide 처리에 의해 유발된 RAW 264.7 세포 및 λ-carrageenan 처리에 의해 유발된 부종 마우스 모델에서 호장근 열수 추출물의 항염증 효과
Anti-inflammatory Effects of Polygonum cuspidatum Hot Water Extract in Lipopolysaccharide-stimulated RAW 264.7 Cells and λ-Carrageenan-induced Paw Edema Mouse Model
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Background: Polygonum cuspidatum, a plant commonly used in traditional medicine, possesses various pharmacological properties, including anti-inflammatory and antioxidant properties. How- ever, understanding whether P. cuspidatum exerts anti-inflammatory effects and elucidating its underlying mechanisms remain limited. In this study, we evaluated the anti-inflammatory effects of P. cuspidatum hot water extract (PWE) using lipopolysaccharide (LPS)-induced RAW 264.7 cells and a λ-carrageenan (λCAR)-induced paw edema C57BL/6 mouse model. Methods and Results: To evaluate the anti-inflammatory efficacy of PWE, a nitric oxide (NO) assay, quantitative reverse transcription polymerase chain reaction, and western blotting were per- formed. At PWE 400 ㎍/㎖, NO generation was reduced by 51.53 ± 1.94%, compared to that using LPS-stimulated RAW 264.7 cells. PWE also inhibited the interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in a dose-dependent manner at the protein and mRNA levels. Additionally, an in vivo experiment was conducted using a λCAR-induced paw edema mouse model. Cytokine analysis in the λCAR-induced paw edema mouse model revealed that it strongly inhibited pro- inflammatory factors. Conclusions: PWE has strong anti-inflammatory effects based on these results. We suggest that PWE can be used as a functional material for anti-inflammation and may be useful for preventing or treating inflammation.
Abstract
Background: Polygonum cuspidatum, a plant commonly used in traditional medicine, possesses various pharmacological properties, including anti-inflammatory and antioxidant properties. How- ever, understanding whether P. cuspidatum exerts anti-inflammatory effects and elucidating its underlying mechanisms remain limited. In this study, we evaluated the anti-inflammatory effects of P. cuspidatum hot water extract (PWE) using lipopolysaccharide (LPS)-induced RAW 264.7 cells and a λ-carrageenan (λCAR)-induced paw edema C57BL/6 mouse model. Methods and Results: To evaluate the anti-inflammatory efficacy of PWE, a nitric oxide (NO) assay, quantitative reverse transcription polymerase chain reaction, and western blotting were per- formed. At PWE 400 ㎍/㎖, NO generation was reduced by 51.53 ± 1.94%, compared to that using LPS-stimulated RAW 264.7 cells. PWE also inhibited the interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in a dose-dependent manner at the protein and mRNA levels. Additionally, an in vivo experiment was conducted using a λCAR-induced paw edema mouse model. Cytokine analysis in the λCAR-induced paw edema mouse model revealed that it strongly inhibited pro- inflammatory factors. Conclusions: PWE has strong anti-inflammatory effects based on these results. We suggest that PWE can be used as a functional material for anti-inflammation and may be useful for preventing or treating inflammation.
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