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pH 변화 및 균질화 처리에 따른 백삼 추출물의 물리화학적 특성 및 항산화활성

Enhancement of Physicochemical Characteristics and Antioxidant Activities of White Ginseng Extract by pH Control and Homogenization Treatment

김현호|박용찬|성봉재|김선익|지무근|이가순
한국약용작물학회|2022.04|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제30권 제2호|pp. 134-144

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키워드

pHHomogenizationWhite GinsengAntioxidant ActivityGinsenosidesAcidic PolysaccharidesProtein Content

초록

Background: The main useful components of ginseng are ginsenosides, acidic polysaccharides, and proteins. The aim of this study was to increase the extraction efficiency of thease useful components by changing the pH and homogenizing using a buffer solution. Methods and Results: White ginseng powder (WGP) was first extracted with a 20 fold solution of citric acid-phosphate buffer (pH 3.0, 3.5, 4.0, 4.5, and 5.0) and distilled water at 80℃ for 5 hours by stirring in 550 rpm, and then each extract was homogenized with speed (0 to 15,000 rpm) and time (0 to 25 min). Total ginsenosides, acidic polysaccharide crude protein content and transmittance of WGP extract homogenized (12,500 rpm, 15 min) at pH 4.0 - pH 4.5 were 1.07 ㎎/㎖ - 1.10 ㎎/㎖, 0.85% - 0.87%, 0.96% - 0.99% and 36.53% - 40.21%, respectively, and were increased due to pH control and homogenization treatment compared to the untreated (distilled water extraction and no homogenization). The antioxidant activity such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferric reducing/antioxidant power (FRAP) assay of the homogenized (12,500 rpm, 15 min) extract in the pH 4.0 - pH 4.5 was also increased compared to the untreated. Conclusions: As a result, the extraction efficiency of useful components in White ginseng was simultaneously enhanced due to pH adjustment and homogenization treatment along with an increase in antioxidant activity.

Abstract

Background: The main useful components of ginseng are ginsenosides, acidic polysaccharides, and proteins. The aim of this study was to increase the extraction efficiency of thease useful components by changing the pH and homogenizing using a buffer solution. Methods and Results: White ginseng powder (WGP) was first extracted with a 20 fold solution of citric acid-phosphate buffer (pH 3.0, 3.5, 4.0, 4.5, and 5.0) and distilled water at 80℃ for 5 hours by stirring in 550 rpm, and then each extract was homogenized with speed (0 to 15,000 rpm) and time (0 to 25 min). Total ginsenosides, acidic polysaccharide crude protein content and transmittance of WGP extract homogenized (12,500 rpm, 15 min) at pH 4.0 - pH 4.5 were 1.07 ㎎/㎖ - 1.10 ㎎/㎖, 0.85% - 0.87%, 0.96% - 0.99% and 36.53% - 40.21%, respectively, and were increased due to pH control and homogenization treatment compared to the untreated (distilled water extraction and no homogenization). The antioxidant activity such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferric reducing/antioxidant power (FRAP) assay of the homogenized (12,500 rpm, 15 min) extract in the pH 4.0 - pH 4.5 was also increased compared to the untreated. Conclusions: As a result, the extraction efficiency of useful components in White ginseng was simultaneously enhanced due to pH adjustment and homogenization treatment along with an increase in antioxidant activity.

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