영지버섯과 아까시흰구멍버섯의 판별을 위한 미토콘드리아 유전체 기반 InDel 마커 개발
Development of Mitocondrial Genome-based InDel Markers to Distinguish between Ganoderma lucidum and Perenniporia fraxinea
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Background: Ganoderma lucidum and Perenniporia fraxinea are morphologically similar, mak- ing them difficult to distinguish when cut into pieces or powdered. Ganoderma lucidum is a more effective medicinal mushroom than P. fraxinea, but it is expensive, so there is a strong possibility that similar shaped mushrooms are mixed and sold together. Therefore, molecular biological mark- ers that can differentiate G. lucidum from P. fraxinea are needed. Methods and Results: We selected two polymorphic insertion-deletion (InDel) sites and one poly- morphic InDel site from the cox1 and rnl genes within the mitochondrial DNA, respectively, and designed primers using flanking sequences that surrounded these polymorphic sites. We performed a polymerase chain reaction (PCR) to validate these markers. Three InDel markers were developed to differentiate between G. lucidum and P. fraxinea: GlPf_INDEL01, GlPf_INDEL02, and GlP- f_INDEL04. Conclusions: The three InDel markers developed in this study combined with a simple PCR anal- ysis easily distinguished G. lucidum from P. fraxinea.
Abstract
Background: Ganoderma lucidum and Perenniporia fraxinea are morphologically similar, mak- ing them difficult to distinguish when cut into pieces or powdered. Ganoderma lucidum is a more effective medicinal mushroom than P. fraxinea, but it is expensive, so there is a strong possibility that similar shaped mushrooms are mixed and sold together. Therefore, molecular biological mark- ers that can differentiate G. lucidum from P. fraxinea are needed. Methods and Results: We selected two polymorphic insertion-deletion (InDel) sites and one poly- morphic InDel site from the cox1 and rnl genes within the mitochondrial DNA, respectively, and designed primers using flanking sequences that surrounded these polymorphic sites. We performed a polymerase chain reaction (PCR) to validate these markers. Three InDel markers were developed to differentiate between G. lucidum and P. fraxinea: GlPf_INDEL01, GlPf_INDEL02, and GlP- f_INDEL04. Conclusions: The three InDel markers developed in this study combined with a simple PCR anal- ysis easily distinguished G. lucidum from P. fraxinea.
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