일천궁에 발생하는 오이모자이크바이러스의 현장 검출을 위한 Lateral Flow Reverse Transcription Recombinase Polymerase Amplification (LF-RT-RPA) 개발
Development of Lateral Flow Reverse Transcription Recombinase Polymerase Amplification (LF-RT-RPA) Assay for On-site Detection of Cucumber Mosaic Virus on Cnidium officinale
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Background: Quality and quantity of Cheongung (Cnidium officinale Makinoi) is reduced by plant viral infections. Therefore, it is necessary to develop effective detection methods for diagno- sis of problematic viruses in Cheongung farms. This study aimed to develop lateral flow reverse transcription recombinase polymerase amplification (LF-RT-RPA) assay for the detection of cucumber mosaic virus (CMV) in C. officinale. Methods and Results: Total RNA was extracted from CMV-infected Cheongung plant and sub- jected to RT-RPA reaction with RPA_CMV-CP-F1/R1 primers targeting the CMV RNA3 sequence. When specificity was examined using lateral flow Immunostrip, experimental and con- trol lines were generated only in the sample from the CMV-infected plant, while control lines were observed in samples from cnidium vein yellowing virus-1, cnidium vein yellowing virus-2, and apple stem grooving virus-infected Cheongung plants. The optimal temperature and reaction time for LF-RT-RPA assay were determined to be 37oC and 15 min. On-site diagnosis carried out to detect CMV in 7 Cheongung plants showing viral-infection symptoms, confirmed six of the plants infected with CMV. Conclusion: CMV, one of the prevalent plant viruses, was effectively detected using LF-RT-RPA assay without the need of any equipment on farms. Detection limit for CMV in C. officinale Maki- noi was up to 100 fg of total RNA, suggesting that the LF-RT-RPA assay developed in this study is suitable for on-site detection of CMV in Cheongung farms.
Abstract
Background: Quality and quantity of Cheongung (Cnidium officinale Makinoi) is reduced by plant viral infections. Therefore, it is necessary to develop effective detection methods for diagno- sis of problematic viruses in Cheongung farms. This study aimed to develop lateral flow reverse transcription recombinase polymerase amplification (LF-RT-RPA) assay for the detection of cucumber mosaic virus (CMV) in C. officinale. Methods and Results: Total RNA was extracted from CMV-infected Cheongung plant and sub- jected to RT-RPA reaction with RPA_CMV-CP-F1/R1 primers targeting the CMV RNA3 sequence. When specificity was examined using lateral flow Immunostrip, experimental and con- trol lines were generated only in the sample from the CMV-infected plant, while control lines were observed in samples from cnidium vein yellowing virus-1, cnidium vein yellowing virus-2, and apple stem grooving virus-infected Cheongung plants. The optimal temperature and reaction time for LF-RT-RPA assay were determined to be 37oC and 15 min. On-site diagnosis carried out to detect CMV in 7 Cheongung plants showing viral-infection symptoms, confirmed six of the plants infected with CMV. Conclusion: CMV, one of the prevalent plant viruses, was effectively detected using LF-RT-RPA assay without the need of any equipment on farms. Detection limit for CMV in C. officinale Maki- noi was up to 100 fg of total RNA, suggesting that the LF-RT-RPA assay developed in this study is suitable for on-site detection of CMV in Cheongung farms.
참고문헌 (72)
- Babu B, "Recombinase polymerase amplification applied to plant virus detection and potential implications", Analytical Biochemistry, 546, pp.72~77 (2018)
- Babujee L, "Optimization of an isothermal recombinase polymerase amplification method for real-time detection of Potato virus Y, O and N types in potato", Journal of Virological Methods, 267, pp.16~21 (2019)
- Baranwal VK, "Applied plant virology: Advances, detection, and antiviral strategies", pp.27~37 (2020), Academic Press
- Belete MT, "Complete genome sequence of cnidium virus 1, a novel betanucleorjadovirus infecting Cnidium officinale", Archives of Virology, 167, pp.973~977 (2022)
- Bujarski J, "ICTV virus taxonomy profile : Bromoviridae", Journal of General Virology, 100, pp.1206~1207 (2019)
- Bujarski J., "Encyclopedia of Virology", pp.260~267 (2021), Elsevier
- 조점덕, "우리나라 고추 바이러스 종류, 병징 및 발생 형태", 식물병 연구, 13(2), pp.75~81 (2007), 한국식물병리학회
- Cho SK, "Anti-inflammatory and analgesic activities of the extracts and fractions of cnidii rhizoma", Korean Journal of Pharmacognosy, 27, pp.282~287 (1996)
- Choi SK, "RT-PCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers", Journal of Virological Methods, 83, pp.67~73 (1999)
- Choi YM, "An investigation of undescribed witches' broom symptom disease caused by mycoplasma-like organism on Bupleurum falcatum, Cnidium officinale and Plantago asiatica in Korea", The Korean Journal of Mycology, 13, pp.49~51 (1985)
- Chung BN, "First report of Cnidium officinale as a natural host plant of apple stem grooving virus in South Korea", Plant Disease, 106, pp.338~338 (2022)
- Clark MF, "Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses", Journal of General Virology, 34, pp.475~483 (1977)
- Crannell ZA, "Equipment-free incubation of recombinase polymerase amplification reactions using body heat", PLOS One, 9, pp.e112146~ (2014)
- Davis RF, "Cucumber mosaic virus isolates seedborne in Pahseolus vulgaris : Serology, host-pathogen relationships, and seed transmission", Phytopathology, 76, pp.999~1004 (1986)
- Devergne JC, "Comparison of direct and indirect ELISA for detecting antigenically related cucumovirus", Journal of Virological Methods, 3, pp.193~199 (1981)
- Edwardson JR, "CRC Handbook of viruses infecting legumes", pp.293~319 (1991), CRC Press
- Francki RIB, "Cucumber mosaic virus-Variation and problems of identification", Acta Horticulturae, 110, pp.167~174 (1980)
- 한경민, "The First Report of Fusarium solani Causing Wilting in Cnidium officinale in Korea", 식물병 연구, 27(2), pp.76~78 (2021), 한국식물병리학회[DOI]
- Honma H, "Complete nucleotide sequence of a new potexvirus, ‘Cnidium virus X’, isolated from Cnidium officinale in Japan", Archives of Virology, 164, pp.1931~1935 (2019)
- Hord MJ, "Field survey of Cucumber mosaic virus subgroup I and II in crop plants in Costa Rica", Plant Disease, 85, pp.952~954 (2001)
- Hsu YC, "A new combination of RT-PCR and reverse dot blot hybridization for rapid detection and identification of potyviruses", Journal of Virological Methods, 128, pp.54~60 (2005)
- Hu JS, "Comparison of dot blot, ELISA and RT-PCR assays for detection of two cucumber mosaic virus isolates infecting banana in Hawaii", Plant Disease, 79, pp.902~906 (1995)
- Hwang J, "Comparision of chemical components of Ligusticum chuanzxiong Hort and Cnidium officinale Makino", Analytical Science and Technology, 11, pp.54~61 (1998)
- Jones RAC, "Control of plant virus diseases", Advances in Virus Research, 67, pp.205~244 (2006)
- 정충렬, "일천궁과 토천궁에서 발생하는 총채벌레류의 분자동정", 한국약용작물학회지, 27(1), pp.17~23 (2019), 한국약용작물학회[DOI]
- Jung CR, "Selection of natural product extracts against Phoma sp. and Colletotrichum gloesporioides occurred in Korean angelica(Angelica gigas Nakai)and cnidii rhizoma(Cnidium officinale Makino)", Proceedings of the Korean Journal of Medicinal Crop Science, 26, pp.30~30 (2018)
- 김덕환, "천궁밭 발생잡초 및 군락특성", Weed & Turfgrass Science, 4(4), pp.279~287 (2015), 한국잔디학회
- 김도현, "Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay", The Plant Pathology Journal, 38(6), pp.665~672 (2022), 한국식물병리학회[DOI]
- 김정수, "2010년 우리나라 주요 작물 바이러스병 발생 상황", 식물병 연구, 17(3), pp.334~341 (2011), 한국식물병리학회
- Kim JY, "Development of a multiplex real-time recombinase polymerase amplification(RPA)assay for rapid quantitative detection of Campylobacter coli and jejuni from eggs and chicken products", Food Control, 73, pp.1247~1255 (2017)
- Na-Kyeong Kim, "Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of Barley Yellow Dwarf Virus in Oat", The Plant Pathology Journal, 36(5), pp.497~502 (2020), 한국식물병리학회[DOI]
- 김나경, "Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips", The Plant Pathology Journal, 38(2), pp.159~166 (2022), 한국식물병리학회[DOI]
- Kim NY, "A portable detection assay for apple stem pitting virus using reverse transcription-recombinase polymerase amplification", Journal of Virological Methods, 274, pp.113747~ (2019)
- Lee CB, "The encyclopeida of Korea plants", pp.583~ (1985), Hyang Mun Sa
- Lee DS, "Complete genome sequences of two apple stem grooving viruses in Cnidium officinale in Korea", Microbiology Resource Announcements, 12, pp.e00902~e00922 (2023)
- Lee HJ, "Rapid and visual detection of Tomato spotted wilt virus using recombinase polymerase amplification combined with lateral flow strips", Molecular and Cellular Probes, 57, pp.101727~ (2021)
- Lee HJ, "Rapid detection of Peach latent mosaic viroid by reverse transcription recombinase polymerase amplification", Molecular and Cellular Probes, 53, pp.101627~ (2020)
- Lee SC, "Multiplex RT-PCR detection of two orchid viruses with an internal control of plant nad5 mRNA", Plant Pathology Bulletin, 15, pp.187~196 (2006)
- Li TT, "Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification(LF-RPA) assay", Frontiers in Cellular and Infection Microbiology, 9, pp.1~ (2019)
- Lillis L, "Non-instrumented incubation of a recombinase polymerase amplification assay for the rapid and sensitive detection of proviral HIV-1 DNA", PLOS One, 9, pp.e108189~ (2014)
- Lobato IM, "Recombinase polymerase amplification : Basics, applications and recent advances", Trends Analytical Chemistry, 98, pp.19~35 (2018)
- Londoño MA, "Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics", Virology Journal, 13, pp.48~ (2016)
- Lu Y, "The detection of ACLSV and ASPV in pear plants by RT-LAMP assays", Journal of Virological Methods, 252, pp.80~85 (2018)
- Mekuria TA, "Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification", Journal of Virological Methods, 205, pp.24~30 (2014)
- Impact assessment based on climate change scenarios (RCP) in apple, grape, mandarin, ginseng, cnidium, and Korean Angelica, "National Institute of Horiticultural and Herbal Science(NIHHS)", National Institute of Horiticultural and Herbal Science. Rural Development Administration, 89~125 (2017)
- 오영지, "천궁(Cnidium officinale Makino) 지상부 추출물의 항산화 활성 평가", 한국약용작물학회지, 18(6), pp.373~378 (2010), 한국약용작물학회
- Ortega-Acosta C, "First report of cucumber mosaic virus subgroup I and II with satellite RNA infecting Nicotiana glauca in Mexico", Journal of Plant Pathology, 101, pp.801~ (2019)
- Owen J, "Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping of RNA3 in eight CMV strains", Virology, 166, pp.495~502 (1988)
- Palukaitis P, "Cucumoviruses", Advances in Virus Research, 62, pp.241~323 (2003)
- Palukaitis P, "Cucumber mosaic virus", Advances in Virus Research, 41, pp.281~348 (1992)
- Park YC, "Complete genome sequence of cnidium virus 2, a novel cytorhabdovirus isolated from Cnidium officinale in South Korea", Archives of Virology, 168, pp.170~ (2023)
- Park YK, "The study on antioxidative effects and quality comparison of Ligusticum chuanxiong and Cnidium officinale(Ⅰ)", The Korea Journal of Herbology, 13, pp.103~114 (1998)
- Peng J, "Detection of Cucumber mosaic virus isolates from banana by one step reverse transcription loop-mediated isothermal amplification", Archives of Virology, 157, pp.2213~2217 (2012)
- Piepenburg O, "DNA detection using recombination proteins", PLOS Biology, 4, pp.e204~ (2006)
- Rao ALN, "Comparative studies on tomato aspermy virus and cucumber mosaic viruses VII. Serological relationships reinvestigated", Virology, 116, pp.318~326 (1982)
- Scott HA, "Serological behavior of cucumber mosaic virus(strain Y)and the virus protein", Virology, 34, pp.79~90 (1968)
- 서영진, "기상요인이 일천궁의 증발산량 변화에 미치는 영향", 한국농림기상학회지, 20(4), pp.366~375 (2018), 한국농림기상학회
- Shaman M, "First record of natural infection of abaca(Musa textilis) with Banana bract mosaic potyvirus in the Philippines", Australian Plant Pathology, 29, pp.69~ (2000)
- Silva G, "Rapid and specific detection of yam mosaic virus by reverse-transcription recombinase polymerase amplification", Journal of Virological Methods, 222, pp.138~144 (2014)
- 송임근, "천궁의 기원과 식별을 위한 분자마커", 대한본초학회지, 24(4), pp.1~8 (2009), 대한본초학회
- Srivastava N, "Rapid diagnosis of cucumber mosaic virus in banana plants using a fluorescence-based real-time isothermal reverse transcription-recombinase polymerase amplification assay", Journal of Virological Methods, 270, pp.52~58 (2019)
- Stevens M, "Comparison of ELISA and RT-PCR for the detection of beet yellows closterovirus in plants and aphids", Journal of Virological Methods, 68, pp.9~16 (1997)
- Takeshita M, "Involvement of cucumber mosaic cucumovirus RNA2 and RNA3 in viral systemic spread in radish plant", Archives of Virology, 143, pp.1109~1117 (1998)
- 엄지나, "당귀, 작약, 지황, 천궁 혼합 발효물의 항산화, 항주름 및 미백 효과", 한국약용작물학회지, 25(3), pp.152~159 (2017), 한국약용작물학회[DOI]
- Wahyuni WS, "Serological and biological variation between and within subgroup I and II strains of cucumber mosaic virus", Plant Pathology, 41, pp.282~297 (1992)
- Yang HJ, "Survey of virus occurrence in Cnidium officinale and Ligustucum chuanxiong", Proceedings of the Korean Journal of Pesticide Science, pp.304~304 (2018)
- Yoo RH, "The complete nucleotide sequence and genome organization of lychnis mottle virus", Archives of Virology, 160, pp.2891~2894 (2015)
- 윤영배, "RCP 기후변화 시나리오에 따른 천궁의 생장 및 생리 활성 변화", 한국약용작물학회지, 30(6), pp.450~461 (2022), 한국약용작물학회
- Zhang S, "Rapid diagnostic detection of plum pox virus in prunus plants by isotheral AmplifyRP using reverse transcription-recombinase polymerase amplification", Journal of Virological Methods, 207, pp.114~120 (2014)
- Zhao C, "Reverse transcription-recombinase polymerase amplification combined with lateral flow strip for detection of rice black-streaked dwarf virus in plants", Journal of Virological Methods, 263, pp.96~100 (2019)
- Zhao L, "Rapid detection of tobacco viruses by reverse transcription loop-mediated isothermal amplification", Archives of Virology, 157, pp.2291~2298 (2012)
- Zhao XJ, "Development of a RT-LAMP method for the rapid detection of cucumber mosaic virus from vegetables", Acta Horticulturae Sinica, 43, pp.1203~1210 (2016)