Nucleopolyhedrovirus Induces Suppressor of Cytokine Signaling in the Beet Armyworm, Spodoptera exigua
2
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
Suppressor of cytokine signaling (SOCS) is known to play a key role as a negative feedback regulator in JAK/STAT signaling cascade in innate immunity. Our laboratory has recently been interested in elucidating the interactions between Spodoptera exigua (Se) and SeNPV. This context leads us to clone and characterize SeSOCS that may have important functions in response to SeNPV infection. Using the RT-PCR and TA cloning approach, we found a partial fragment (416 bp) of SeSOCS. Blast search and multiple alignment data showed that it has a homology to various insects such as Anopheles gambiae (78%), Aedes aegypti (75%), Drosophila melanogastar (77%), Mus musculus (69%), and Homo sapiens (69%). Temporal induction patterns of SeSOCS were analysed after being immune-challenged with either NPV or laminarin. It showed that the level of SeSOCS mRNA was strongly induced in a biphasic manner in response to SeNPV and laminarin, respectively. It seems that SOCS, a negative regulator of JAK/STAT signaling system is also present in S. exigua and may play a role in innate immunity albeit its precise role should be further elucidated at the molecular and cellular level in the early phase of SeNPV infection in larvae.
참고문헌 (13)
- "The role of suppressors of cytokine signaling proteins in regulation of the immune response", 22, pp.503~529 (2004)
- "Anopheles gambiae Ag-STAT, a new insect member of the STAT family, is activated in response to bacterial infection.", 18, pp.959~967 (1999)
- "lessons from insect model systems and implications for vertebrate innate immunity and vaccine development", 30, pp.429~442 (2000)
- "The JAK", STAT signaling pathway is required but not sufficient for the antiviral response of drosophila, 2005, pp.6, 946~953
- "Molecular interactions between Anopheles stephensi midgut cells and Plasmodium berghei the time bomb theory of ookinete invasion of mosquitoes", pp.6030~6040 (2000)
- "Implications of time bomb model of ookinete invasion of midgut cells", 32, pp.1311~1316 (2002)
- "Innate immune responses of a lepidopteran insect", 198, pp.97~105 (2004)
- "Characterization of two mosquito STATs Differential regulation of tyrosine phosphorylation and DNA binding activity by lipopolysaccharide treatment and by Japanese encephalitis virus infection", 279, pp.3308~3317 (2004)
- "STAT-like DNA-binding activity in Spodoptera frugiperda cells", 225, pp.562~569 (1996)
- "flexible strategies for multiple sequence alignment aided by quality analysis tools", 25, pp.4876~4882 (1997)
- "The complex interplay between mosquito positive and negative regulators of Plasmodium development", 8, pp.415~421 (2005)
- "RNA interference directs innate immunity against viruses in adult Drosophila", 312, pp.452~454 (2006)
- "Induction of suppressor of cytokine signaling-3 by herpes simplex virus type 1 confers efficient viral replication", 338, pp.173~181 (2005)