Comparative Evaluation of Fibrin for Bone Regeneration in Critical Size Calvarial Defects
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Natural biopolymers such as collagen and fibrin have beenwidely used in bone regenerative applications. Despite thefrequent use, their comparative biological propertiesis arelargely unknown. In a previous study, we found thesuperiority of fibrin to collagen in the adsorption of serumproteins and the proliferation and differentiation of culturedosteoblasts. In this study, we used an in vivo model toevaluate how effectively fibrin supports bone regeneration,as compared with collagen. Collagen and fibrin were placedin critical size defects made on rat calvarial bones. Comparedwith collagen, fibrin supported substantially more new bonetissue formation, which was confirmed by micro-CTmeasurement and histological analyses. The cells in theregenerative tissues of the fibrin-filled defects wereimmunostained strongly for Runx2, while collagen-placeddefects were stained weakly. These in vivo resultsdemonstrate that fibrin is superior to collagen in supporting bone regeneration.
Abstract
Natural biopolymers such as collagen and fibrin have beenwidely used in bone regenerative applications. Despite thefrequent use, their comparative biological propertiesis arelargely unknown. In a previous study, we found thesuperiority of fibrin to collagen in the adsorption of serumproteins and the proliferation and differentiation of culturedosteoblasts. In this study, we used an in vivo model toevaluate how effectively fibrin supports bone regeneration,as compared with collagen. Collagen and fibrin were placedin critical size defects made on rat calvarial bones. Comparedwith collagen, fibrin supported substantially more new bonetissue formation, which was confirmed by micro-CTmeasurement and histological analyses. The cells in theregenerative tissues of the fibrin-filled defects wereimmunostained strongly for Runx2, while collagen-placeddefects were stained weakly. These in vivo resultsdemonstrate that fibrin is superior to collagen in supporting bone regeneration.
참고문헌 (23)
- Lynch MP, "The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: modification of expression of genes supporting cell growth, adhesion, and extracellular matrix mineralization", Exp Cell Res, 216, pp.35~45 (1995)
- Mizuno M, "Type I collagen-induced osteoblastic differentiation of bone-marrow cells mediated by collagen-alpha2beta1 integrin interaction", J Cell Physiol, 184, pp.207~213 (2000)
- Moursi AM, "Interactions between integrin receptors and fibronectin are required for calvarial osteoblast differentiation in vitro", J Cell Sci, 110, pp.2187~2196 (1997)
- Knight CG, "The collagen-binding A-domains of integrins alpha(1)beta(1)and alpha(2)beta(1)recognize the same specific amino acid sequence, GFOGER, in native(triple-helical)collagens", J Biol Chem, 275, pp.35~40 (2000)
- Oh JH, "Suppression of Runx2 protein degradation by fibrous engineered matrix", Biomaterials, 32, pp.5826~5836 (2011)
- Li WJ, "Electrospun nanofibrous structure : a novel scaffold for tissue engineering", J Biomed Mater Res, 60, pp.613~621 (2002)
- Woo KM, "Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization", Biomaterials, 28, pp.335~343 (2007)
- Ma PX, "Biomimetic materials for tissue engineering", Adv Drug Delivery Rev, 60, pp.184~198 (2008)
- Breen A, "Fibrin as a Delivery System for Therapeutic Drugs and Biomolecules", Tissue Eng Part B-Re, 15, pp.201~214 (2009)
- Amrani DL, "Wound healing. Role of commercial fibrin sealants", Ann N Y Acad Sci, 936, pp.566~579 (2001)
- Clark RA, "Fibrin is a many splendored thing", J Invest Dermatol, 121, pp.21~22 (2003)
- Mosesson MW, "The structure and biological features of fibrinogen and fibrin", Ann N Y Acad Sci, 936, pp.11~30 (2001)
- Alston SM, "In vivo efficacy of a new autologous fibrin sealant", J Surg Res, 146, pp.143~148 (2008)
- Flanagan TC, "The in vitro development of autologous fibrin-based tissue-engineered heart valves through optimised dynamic conditioning", Biomaterials, 28, pp.3388~3397 (2007)
- 오정환, "Comparative evaluation of the biological properties of fibrin for bone regeneration", BMB Reports, 47(2), pp.110~114 (2014), 생화학분자생물학회
- Jung HM, "Modulation of the resorption and osteoconductivity of alpha-calcium sulfate by histone deacetylase inhibitors", Biomaterials, 31, pp.29~37 (2010)
- Oh JH, "The effects of the modulation of the fibronectin-binding capacity of fibrin by thrombin on osteoblast differentiation", Biomaterials, 33, pp.4089~4099 (2012)
- Collet JP, "Influence of fibrin network conformation and fibrin fiber diameter on fibrinolysis speed : dynamic and structural approaches by confocal microscopy", Arterioscler Thromb Vasc Biol, 20, pp.1354~1361 (2000)
- Davis HE, "Supplementation of fibrin gels with sodium chloride enhances physical properties and ensuing osteogenic response", Acta Biomater, 7, pp.691~699 (2011)
- Komori T, "Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts", Cell, 89, pp.755~764 (1997)
- Prince M, "Expression and regulation of Runx2", Cbfa1 and osteoblast phenotypic markers during the growth and differentiation of human osteoblasts, 2001, pp.80, 424~440
- Lee MH, "Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor beta1 in C2C12 myogenic cells coincides with suppression of the myogenic phenotype but is not sufficient for osteoblast differentiation", J Cell Biochem, 73, pp.114~125 (1999)
- Xiao G, "Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells", J Bone Miner Res, 17, pp.101~110 (2002)