본문 바로가기
KERD
논문KCI 등재

Recombinant Expression and Enzyme Activity of Chymotrypsin-like protease from Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae)

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Chymotrypsin serine protease is one of the main digestive proteases in the midgut of and is involved in various essential processes. In a previous study, a gene encoding a chymotrypsin-like protease, Hi-SP1,was cloned from the larvae of Hermetia illucens and characterized. In this study, we produced the recombinant chymotrypsin-like protease Hi-SP1 in Escherichia coli cells. The molecular weight of the recombinant Hi-SP1 was estimated to be approximately 26 kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western-blotting. Chymotrypsin activity was detected when AAPF was used as the substrate. Examination of the effects of temperature and pH revealed that the proteolytic activity of recombinant Hi-SP1 decreased markedly at temperatures above 30oC, and the optimum pH was found to be 10.0.

Abstract

Chymotrypsin serine protease is one of the main digestive proteases in the midgut of and is involved in various essential processes. In a previous study, a gene encoding a chymotrypsin-like protease, Hi-SP1,was cloned from the larvae of Hermetia illucens and characterized. In this study, we produced the recombinant chymotrypsin-like protease Hi-SP1 in Escherichia coli cells. The molecular weight of the recombinant Hi-SP1 was estimated to be approximately 26 kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western-blotting. Chymotrypsin activity was detected when AAPF was used as the substrate. Examination of the effects of temperature and pH revealed that the proteolytic activity of recombinant Hi-SP1 decreased markedly at temperatures above 30oC, and the optimum pH was found to be 10.0.

참고문헌 (25)

  1. Balti R, "Chymotrypsin from the hepatopancreas of cuttlefish (Sepia officinalis) with high activity in the hydrolysis of long chain peptide substrates: Purification and biochemical characterisation", Food Chem, 130, pp.475~484 (2012)
  2. Blow DM, "The structure of chymotrypsin, Vol. 3" (1971), Academic Press
  3. Bondari K, "Soldier fly larvae as feed in commercial fish production", Aquaculture, 24, pp.103~109 (1981)
  4. Diener S, "Conversion of organic material by black soldier fly larvae: establishing optimal feeding rates", Waste Manage Res, 27, pp.603~610 (2009)
  5. Gráf L, "Chymotrypsin" (1998), Academic Press
  6. Herrero S, "Identification and recombinant expression of a novel chymotrypsin from Spodoptera exigua", Insect Biochem Molec, 35, pp.1073~1082 (2005)
  7. James MT, "The genus Hermetia in the United States (Diptera: Stratiomyidae)", Bull Brooklyn Entomol Soc, 30, pp.165~170 (1935)
  8. 김종길, "국내 서식하는 아메리카동애등에(Hermetia illucens)의 생태 특성", 한국응용곤충학회지, 47(4), pp.337~343 (2008), 한국응용곤충학회
  9. Kim JI, "Newly recording two exotic insects species from Korea", J Kor Biota, 2, pp.223~225 (1997)
  10. Wontae Kim, "Characterization of the molecular features and expression patterns of two serine proteases in Hermetia illucens (Diptera: Stratiomyidae) larvae", BMB Reports, 44(6), pp.387~392 (2011), 생화학분자생물학회
  11. Klomklao S, "Trypsin from the pyloric caeca of bluefish (Pomatomus saltatrix)", Comp Biochem Phys B, 148, pp.382~389 (2007)
  12. Lee MJ, "Endoproteases from the midgut of larval Spodoptera littoralis include a chymotrypsin-like enzyme with an extended binding-site", Insect Biochem Molec, 25, pp.49~61 (1995)
  13. Lord WD, "The black soldier fly Hermetia illucens (Diptera: Stratiomyidae) as a potential measure of human postmortem interval: observations and case histories", J Forensic Sci, 39, pp.215~222 (1994)
  14. Mazumdar-Leighton S, "Identification of six chymotrypsin cDNAs from larval midguts of Helicov-erpa zea and Agrotis ipsilon feeding on the soybean (Kunitz) trypsin inhibitor", Insect Biochem Molec, 31, pp.633~644 (2001)
  15. McCallan E, "Hermetia illucens (L.) (Diptera: Stratiomyidae), a cosmopolitan American species long established in Australia and New Zealand", Entomol Mo Mag, 109, pp.232~234 (1974)
  16. Newton GL, "Dried Hermetia illucens larvae meal as a supplement for swine", J Anim Sci, 44, pp.395~400 (1977)
  17. Noriega FG, "A molecular view of trypsin synthesis in the midgut of Aedes aegypti", J Insect Physiol, 45, pp.613~620 (1999)
  18. Rawlings ND, "Families of Serine Peptidases", Proteolytic Enzymes: Serine and Cysteine Peptidases, 244, pp.19~61 (1994)
  19. Sheppard DC, "A value added manure management system using the black soldier fly", Bio resource Tech, 50, pp.275~279 (1994)
  20. Simpson, BK, "Trypsin from Greenland cod, Gadus ogac. Isolation and comparative properties", Comp Biochem Physiol, 79 (1984)
  21. Terra WR, "Insect Digestive Enzymes - Properties, Compartmentalization and Function", Comp Biochem Phys B, 109, pp.1~62 (1994)
  22. Terra WR, "Digestive enzymes" (1996), Chapman and Hall
  23. Tomberlin JK, "Factors influencing mating and oviposition of black soldier flies (Diptera: Stratiomyidae) in a colony", J Entomol Sci, 37, pp.345~352 (2002)
  24. Tomberlin JK, "Black soldier fly (Diptera: Stratiomyidae) colonization of pig carrion in South Georgia", J Forensic Sci, 50, pp.152~153 (2005)
  25. Tomberlin JK, "Black Development of the black soldier fly (Diptera: Stratiomyidae) in relation to temperature", Environ Entomol, 38, pp.930~934 (2009)

관련 논문AI추천

Characterization of the molecular features and expression patterns of two serine proteases in Hermetia illucens (Diptera: Stratiomyidae) larvae고영호, 김아영, 김원태, 박관호, 박영한, 배성우, 이상범, 최영철, 한상미생화학분자생물학회 · BMB Reports2011 · 제44권 제6호Molecular Cloning, Gene Structure, Expression and Enzyme Activity of a Serine Protease from Laccotrephes japonensis (Hemiptera; Nepidae)남승희, 노시갑, 황재삼, Kwan-Ho Park, Youngcheol Choi한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2012 · 제25권 제2호Screening and Characterization of a Novel Cellulase Gene from the Gut Microflora of Hermetia illucens Using Metagenomic Library구본성, 윤상홍, 이창묵, 한범수, Joon-Soo Sim, So-Hyeon Seo, Soo-Jin Kim, Young-Seok Lee한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2014 · 제24권 제9호Identification and Characterization of a New Alkaline Thermolysin-Like Protease, BtsTLP1, from Bacillus thuringiensis Serovar Sichuansis Strain MC28Frits Goedegebuur, Helong Hao, Keya Zhang, Lilia Babe, Xiaogang Gu, Zhenghong Zhang, Zhengzheng Zou, Zhiyong Xie, Zhongmei Tang한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2015 · 제25권 제8호Purification and Characterization of a Novel Extracellular Thermostable Alkaline Protease from Streptomyces sp. M30Jue Wang, Linfeng Luogong, Qiongzhen Chen, Shuhuan Li, Weiliang Dong, Yan Huang, Yan Xin, Yicheng Wang, Zhibin Sun, Zhongli Cui한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2015 · 제25권 제11호Purification, Characterization, and Cloning of a Cold-Adapted Protease from Antarctic Janthinobacterium lividum김수미, 김현도, 최종일한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제3호

이 간행물의 다른 논문 (11건)