PCR-Based Detection of Densovirus Infection in Silkworm (Bombyx mori L.)
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
Two pairs of DNA primers were designed for the detection of the Zhenjiang (China) strain of Bombyx mori densonucleosis virus (BmDNV-Z). These primers were designed from the nucleotide sequence of major structural protein gene (putative VD1-ORF2). PCR amplification was attempted from different issues (including silk gland, blood, skin and midgut) and feces of the silkworm which infected wit BmDNV-Z were amplified by PCR. Both of the primers gave expected size of in the DNA bands from midgut and feces, but not in the DNA of silk gland, blood and skin.The two bands were sequenced, and their sequence were same as the sequence designed for. BmDNV-Z could be successfully detected in single silkworm after it was infected for 12 hrs, and could not be detected before 9 hrs after infected.
참고문헌 (12)
- "Terminalstructure of a densovirus implies a hairpin transfer replicationwhich is similar to the model for AAV. Virology 179", pp.57~63 (1990)
- ". Molecular cloningand characterization of Bombyx densovirus genomicDNA. Arch. Virology 93", pp.139~146 (1987)
- "Organization and nucleotide sequence of adensovirus genome imply a host-dependent evolution of parvoviruses.J. Virology 61", pp.553~560 (1987)
- "Studies on thr infectionsite by the densonucleosis virus in the diseased silkworm", pp.93~98 (1985)
- "The development of a densonucleosisvirus isolated from silkworm larvae", pp.219~222 (1983)
- "Molecular biology of insect viruses" (1998), China agricultural scientech press
- "Serological diagnosesof the densonucleosis disease of Bombyx mori. ActaSericologica Sinica 15", pp.217~222 (1989)
- "Theserological relationship of Bombyx mori densonucleosisvirus from China and Japan. Acta Sericologica Sinica 11", pp.241~242 (1985)
- "Histopathological features andpathogenicity of a densonucleosis virus of the silkworm isolated from sericultural farms in Yamanashiprefecture. J. Seric. Sci. Jpn 52", pp.400~405 (1983)
- "Differencein serological characteristics of densonucleosisviruses in the silkworm Bombyx mori. J. Seric. Sci. Jpn 55", pp.75~76 (1986), T. Shimizu and H. Seki
- "Cloning of the major structural protein gene of densovirus (Zhenjiang Isolate) in silkworm, Bombyx mori and its expression in Escherichia coli", 20, pp.279~282 (2004)
- "Bombyx mori densovirus 3 isolate VD1, complete genome" (2005)
