Molecular Characterization of Small Heat Shock Protein(hsp20.8A) from the Silkworm, Bombyx mori
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To define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from diapausing eggs and diapause activated eggs were compared with differential expression using cDNA array. Among those clones, mRNA transcript from hsp 20.8A, which was expressed at a high level in diapausing eggs that had been incubated at 25oC for 30 days after oviposition, whereas, in the eggs exposed to 15oC for 30 days, 5oC for 60 days, the expression of mRNA decreased. On the other hand, the expression of mRNA during embryogenesis observed abundantly at 4 to 6 day after heat–HCl treatment and later at 9 to 10 day after just before hatching. This result was suggested for us that hsp20.8A was expressed in response to embryogenesis as well as physical stress.
Abstract
To define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from diapausing eggs and diapause activated eggs were compared with differential expression using cDNA array. Among those clones, mRNA transcript from hsp 20.8A, which was expressed at a high level in diapausing eggs that had been incubated at 25oC for 30 days after oviposition, whereas, in the eggs exposed to 15oC for 30 days, 5oC for 60 days, the expression of mRNA decreased. On the other hand, the expression of mRNA during embryogenesis observed abundantly at 4 to 6 day after heat–HCl treatment and later at 9 to 10 day after just before hatching. This result was suggested for us that hsp20.8A was expressed in response to embryogenesis as well as physical stress.
참고문헌 (20)
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