본문 바로가기
KERD
논문KCI 등재

Optimal Cold Temperature for the Artificial Hibernation of Bombus ignitus Queen Bumblebees

2

조회

0

다운로드

5

인용

0

참고문헌

키워드

초록

Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of -2.5°C, 0°C, 2.5°C and 5°C with constant humidity >80%, the queens stored at 2.5°C exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at 0°C, 5°C, 7.5°C and 12.5°C. At 2.5°C the colony developmental characteristics after diapause were 1.2- to 1.5-fold greater than those when queens were stored at 5°C. Thus, 2.5°C and 70% R.H. were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens.

Abstract

Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of -2.5°C, 0°C, 2.5°C and 5°C with constant humidity >80%, the queens stored at 2.5°C exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at 0°C, 5°C, 7.5°C and 12.5°C. At 2.5°C the colony developmental characteristics after diapause were 1.2- to 1.5-fold greater than those when queens were stored at 5°C. Thus, 2.5°C and 70% R.H. were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens.

참고문헌 (42)

  1. Alford DV, "A study of the hibernation of bumblebees (Hymenoptera: Bombidae) in southern England", J Anim Ecol, 38, pp.149~170 (1969)
  2. Alford DV, "Studies on the fat body of adult bumble-bees", J Apic Res, 8, pp.37~48 (1969)
  3. Alford DV, "Bumblebees", pp.352~ (1975), Davies-Poynter
  4. Andrewartha HG, "Diapause in relation to the ecology of insects", Bio Rev, 27, pp.50~107 (1952)
  5. Asada S, "Studies on year-round rearing of Japanese native bumblebees(Bombus spp. )for buzz-foraging crop pollination", Bull-Kanagawa Prefect Agric Res Inst, 144, pp.3~18 (2004)
  6. Beekman M, "Diapause survival and post-diapause performance in bumblebee queens(Bombus terrestris)", Entomol Exp App, 89, pp.207~214 (1998)
  7. Beekman M, "Does the diapause experience of bumblebee queens Bombus terrestris affect colony characteristics", Ecol Entomol, 25, pp.1~6 (2000)
  8. Beekman MP, "Artificial rearing of bumble bee(Bombus terrestris)selects against heavy queens", J Api Res, 39, pp.61~65 (2000)
  9. Chino H, "Carbohydrate metabolism in the diapause egg of the silkworm, Bombyx mori. II. Conversion of glycogen into sorbitol and glycerol during diapause", J Insect Physiol, 2, pp.1~2 (1958)
  10. Crozier AJG, "Supradian and infrandian cycle in oxygen uptake of diapausing pupae of Pieris brassicae", J Insect Physiol, 25, pp.575~582 (1979)
  11. Denlinger DL, "Rates and cycles of oxygen consumption during pupal diapause in Sarcophaga flesh flies", J Insect Physiol, 18, pp.871~882 (1972)
  12. Dag A, "Comparison between the effectiveness of honeybee(Apis millifera)and bumblebee(Bombus terrestris)as pollinators of greenhouse sweet pepper(Capsicum annuum)", Am Bee J, 141, pp.447~448 (2001)
  13. Denlinger DL, "Regulation of diapauses", Annu Rev Entomol, 47, pp.93~122 (2002)
  14. Duchateau MJ, "Development and reproductive strategies in Bombus terrestris colonies", Behavior, 107, pp.186~207 (1988)
  15. Ellingsen IJ, "Oxygen consumption in active and quiescent protonymphs of the American house-dust mite", J Insect Physiol, 24, pp.13~16 (1978)
  16. May ML, "Oxygen consumption by adult colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae", J Insect Physiol, 35, pp.797~804 (1989)
  17. Free JB, "Insect pollination of crops", pp.684~ (1993), Academic Press
  18. Greenleaf S, "Wild bee species increase tomato production but respond differently to surrounding land use in Northern California", Biol Conserv, 133, pp.81~87 (2006)
  19. Heinrich B, "Bumblebee economics" (1979), Harvard University Press
  20. Hoem SN, "Weight and life length of hibernation bumblebee queens (Hymenoptera: Bombidae) under controlled conditions", Ent Scand, 3, pp.313~320 (1972)
  21. Horber E, "Beitrag zur Domestifikationsversuche mit Hummeln(Bombus latr)", Albredht Thaer-Arch, 5, pp.282~304 (1961)
  22. Kai H, "Attempts to elucidate insect diapause development characterized by the potentiation of physiological development during exposure to low temperature", J Seric Sci Jpn, 52, pp.283~289 (1983)
  23. Kai H, "Fixed minimum duration of chilling critical to the termination of diapause, and effects of longer chilling on hatching mode in eggs of Bombyx mori", J Seric Sci Jpn, 64, pp.132~141 (1995)
  24. Mansigh A, "Physiological classification of dormancy in insects", Can Entomol, 103, pp.983~1009 (1971)
  25. May ML, "Oxygen consumption by adult colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)", J Insect Physiol, 35, pp.797~804 (1989)
  26. Michener CD, "The bees of the world" (2000), Johns Hopkins University Press
  27. Minitab Incorporated Company, "Minitab user’s guide", ~ (2000)
  28. Morandin LA, "Wild bee abundance and seed production in conventional, organic, and genetically modified canola", Ecol Appl, 15, pp.871~881 (2005)
  29. Röseler PF, "Effects of carbon dioxide and brain cauterization on corpora allata activity and oogenesia in bumblebees(Bombus hypnorum and Bombus terrestris)", Zool Jb Physiol, 88, pp.237~246 (1984)
  30. Scheniderman HA, "The physiology of insect diapause VII. The respiratory metabolism of the Cecropia silkworm, during diapause and development", Biol Bull, 105, pp.334~ (1953)
  31. van den Eijnde, "Method for rearing Bombus terrestris continuously and the production of bumblebee colonies for pollination purposes", Acta Horticul, 288, pp.154~158 (1991)
  32. Velthuis HHW, "A century of advances in bumblebee domestication and the economic and environmental aspects of its commercialization for pollination", Apidologie, 38, pp.421~441 (2006)
  33. Waku, Y, "Oxygen consumption during diapause in the Lepidopterous pupae-Physiological and autecological significance of its U shaped curve", Sci Rep Tohoku Univ, 28, pp.298~314 (1965)
  34. Winfree R, "Effect of human disturbance on bee communities in a forested ecosystem", Conserv Biol, 21, pp.213~223 (2007)
  35. Yaginuma T, "Malate-aspartate cycles as an effective hydrogen shuttle at the termination of diapause in the eggs of Bombyx mori", J Insect Physiol, 16, pp.677~685 (1986)
  36. Yaginuma T, "Oxygen consumption in relation to sorbitol utilization at the termination of diapause eggs of the silkworm, Bombyx mori", J Insect Physiol, 45, pp.621~627 (1999)
  37. Yoon HJ, "Temperature and humidity favorable for colony development of the indoor-reared bumblebee, Bombus ignitus", Appl Entomol Zool, 37, pp.419~423 (2002)
  38. 윤형주, "Colony Developmental Characteristics of the Bumblebee Queen, Bombus ignitus by the First Oviposition Day", International Journal of Industrial Entomology, 8(2), pp.139~143 (2004), 한국잠사학회
  39. 윤형주, "Influence of Chilling Duration on Oxygen Consumption and Hatchability in Eggs of the Silkworm, Bombyx mori", International Journal of Industrial Entomology, 8(1), pp.73~76 (2004), 한국잠사학회
  40. 윤형주, "The Flight of the Bumblebee Queen, Bombus terrestris, After Diapause Termination Affects to Oviposition and Colony Development", International Journal of Industrial Entomology, 9(2), pp.241~247 (2004), 한국잠사학회
  41. 윤형주, "The Effect of Antiseptic and Sugar Solution on Colony Development of the Bumblebees, Bombus ignitus and B. terrestris", International Journal of Industrial Entomology, 11(1), pp.43~48 (2005), 한국잠사학회
  42. Hyung Joo Yoon, "Chilling Temperature and Humidity to Break Diapause of the Bumblebee Queen Bombus terrestris", International Journal of Industrial Entomology, 20(2), pp.93~98 (2010), 한국잠사학회

관련 논문AI추천

Optimal Timing and Duration of Cold Application for Breaking Diapause in Queens of the Bumblebee Bombus ignitus윤형주, 이경용한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2014 · 제28권 제2호Chilling Temperature and Humidity to Break Diapause of the Bumblebee Queen Bombus terrestris윤형주, 황재삼, In Gyun Park, Kyeong Yong Lee한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2010 · 제20권 제2호A Combination Method of CO2-Narcosis and Cold Treatment for Breaking Diapause of Bombus ignitus and Bombus terrestris Bumblebee Queens윤형주, 이경용한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2014 · 제28권 제2호Wake-up Treatments for Improving Oviposition and Colony Development of the Bumblebees Bombus ignitus and B. terrestris윤형주, In Gyun Park, Kyeong Yong Lee, Samg Beom Lee한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2010 · 제20권 제1호Agricultural Utilization and Year-Round Rearing Techniques of Bumblebees in Korea윤형주, In Gyun Park, Kyeong Yong Lee, Mi Ae Kim한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2011 · 제22권 제2호Temperature, Photoperiod and Illumination for Mating of the European Bumblebee, Bombus terrersisHyung Joo Yoon, In Gyun Park, Kyeong yong Lee, Kyung Choi, Sam Eun Kim, Samg Beom Lee한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2008 · 제16권 제2호

이 간행물의 다른 논문 (7건)