Identification of a novel immune-related gene in the immunized black soldier fly, Hermetia illucens (L.)
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The larvae of Hermetia. illucens have a high probability of coming into contact with microorganisms such as bacteria and fungi. Therefore, the survival of H. illucens is primarily the protection of their own against microbial infection. This effect depends on the development of the innate immune system. Antimicrobial Peptides (AMPs) exhibit antimicrobial activity against other bacterial strains and can provide important data to understand the basis of the innate immunity of H. illucens. In this study, we injected larvae with Enterococcus. faecalis (gram-positive bacteria) and Serratia. marcescens as (gram-negative bacteria) to test the hypothesis that H. illucens is protected from infection by its immune-related gene expression repertoire. To identify the inducible immune-related genes, we performed and cataloged the transcriptomes by RNA-Seq analysis. We compared the transcriptomes of whole larvae and obtained a DNA fragment of 465 bp including the poly (A) tail by RACE as a novel H. illucens immune-related gene against bacteria. A novel target mRNA expression was higher in immunized larvae with E. faecalis and S. marcescens groups than non-immunized group. We expect our study to provide evidence that the global RNA-Seq approach allowed for the identification of a gene of interest which was further analyzed by quantitative RT-PCR, together with genes chosen from the available literature.
Abstract
The larvae of Hermetia. illucens have a high probability of coming into contact with microorganisms such as bacteria and fungi. Therefore, the survival of H. illucens is primarily the protection of their own against microbial infection. This effect depends on the development of the innate immune system. Antimicrobial Peptides (AMPs) exhibit antimicrobial activity against other bacterial strains and can provide important data to understand the basis of the innate immunity of H. illucens. In this study, we injected larvae with Enterococcus. faecalis (gram-positive bacteria) and Serratia. marcescens as (gram-negative bacteria) to test the hypothesis that H. illucens is protected from infection by its immune-related gene expression repertoire. To identify the inducible immune-related genes, we performed and cataloged the transcriptomes by RNA-Seq analysis. We compared the transcriptomes of whole larvae and obtained a DNA fragment of 465 bp including the poly (A) tail by RACE as a novel H. illucens immune-related gene against bacteria. A novel target mRNA expression was higher in immunized larvae with E. faecalis and S. marcescens groups than non-immunized group. We expect our study to provide evidence that the global RNA-Seq approach allowed for the identification of a gene of interest which was further analyzed by quantitative RT-PCR, together with genes chosen from the available literature.
참고문헌 (19)
- Calvino MA, "Spatial-specific action of serotonin within the leech midbody ganglion", J Comp Physiol A Neuroethol Sens Neural Behav Physiol, 194(6), pp.523~531 (2008)[DOI]
- Choi WH, "Novel antibacterial peptides induced by probiotics in Hermetia illucens (Diptera: Stratiomyidae) larvae", Entomol Res (2018)
- Dang XL, "Purification and characterization of an antimicrobial peptide, insect defensin, from immunized house fly (Diptera: Muscidae)", J Med Entomol, 47, pp.1141~1145 (2010)[DOI]
- Elhag O, "Screening, Expression, Purification and Functional Characterization of Novel Antimicrobial Peptide Genes from Hermetia illucens (L.)", PLoS One, 12(1), pp.e0169582~ (2017)[DOI]
- Erickson MC, "Reduction of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis in chicken manure by larvae of the black soldier fly", J Food Prot, 67(4), pp.685~690 (2004)[DOI]
- Feng GJ, "Study of anticoagulant activity of ethanol extracts from leech in vitro", Zhong Yao Cai, 30(8), pp.909~911 (2007)
- Holaskova E, "Antimicrobial peptide production and plant-based expression systems for medical and agricultural biotechnology", Biotechnol Adv, 33(6), pp.1005~1023 (2015)[DOI]
- 이준하, "Anticancer Activity of the Antimicrobial Peptide Scolopendrasin VII Derived from the Centipede, Scolopendra subspinipes mutilans", Journal of Microbiology and Biotechnology, 25(8), pp.1275~1280 (2015), 한국미생물·생명공학회[DOI]
- Lee J, "cDNA cloning and molecular characterization of a defensin-like antimicrobial peptide from larvae of Protaetia brevitarsis seulensis (Kolbe)", Mol Biol Rep, 43(5), pp.371~379 (2016)[DOI]
- Liu Q, "Black soldier fly (Diptera: Stratiomyidae) larvae reduce Escherichia coli in dairy manure", Environ Entomol, 37(6), pp.1525~1530 (2008)[DOI]
- 박순익, "Detection of antimicrobial substances from larvae of the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae)", Entomological Research, 44(2), pp.58~64 (2014), 한국곤충학회
- Park SI, "Purification and characterization of a novel antibacterial peptide from black soldier fly (Hermetia illucens) larvae", Dev Comp Immunol, 52(1), pp.98~106 (2015)[DOI]
- Singh NK, "Vitellogenin from the silkworm, Bombyx mori: an effective anti-bacterial agent", PLoS One, 8(9), pp.e73005~ (2013)[DOI]
- Singh CP, "Characterization of antiviral and antibacterial activity of Bombyx mori seroin proteins", Cellular Microbiol, 16(9), pp.1354~1365 (2014)[DOI]
- Thompson JD, "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Res, 22(22), pp.4673~4680 (1994)[DOI]
- Wei L, "Purification and characterization of a novel defensin from the salivary glands of the black fly, Simulium bannaense", Parasit Vectors, 8, pp.71~ (2015)[DOI]
- Wei HM, "Antimicrobial Properties of an Immunomodulator ¨¢15 kDa Human Granulysin", PLoS One, 11(6), pp.e0156321~ (2016)[DOI]
- Xu XL, "Effects of centipede extracts on normal mouse and S180, H22 bearing mouse", Zhong Yao Cai, 33(4), pp.499~503 (2010)
- Zhou YQ, "Effect of centipede extract on cervical tumor of mice and its mechanism", Zhong Yao Cai, 34(6), pp.859~864 (2011)