Sericin and D-Serine: Exploring Their Role in Bone Health Through Osteoclast Regulation and Serum Metabolism
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Sericin, a silk-derived protein rich in L-serine, exhibits potential bioactivity in modulating bone metabolism. This study investigated the amino acid composition of sericin from various Bombyx mori varieties, its effects on osteoclast activity, and its impact on serum D-serine levels. Amino acid composition analysis revealed significant variations among Bombyx mori varieties, with sericin from different sources containing high levels of L-serine, suggesting diverse bioactive potential. The role of D-serine, a derivative of L-serine, in osteoclast differentiation was examined using RAW264.7 cells. Pretreatment with D-serine significantly suppressed the expression of tartrate-resistant acid phosphatase (TRAP) induced by RANKL and M-CSF, indicating its inhibitory effect on osteoclast activity. Serum analysis following sericin administration in healthy rats demonstrated that a single dose of sericin (25 mg/kg) significantly increased serum D-serine levels compared to the control group (p < 0.001). Although no significant differences were observed among groups fed with varying concentrations of sericin over three days, higher baseline serum D-serine levels were noted in the higher-dose groups. These findings highlight sericin's potential as a functional food component for bone health, driven by its high L-serine content and the osteoclast-inhibitory effects of D-serine.
Abstract
Sericin, a silk-derived protein rich in L-serine, exhibits potential bioactivity in modulating bone metabolism. This study investigated the amino acid composition of sericin from various Bombyx mori varieties, its effects on osteoclast activity, and its impact on serum D-serine levels. Amino acid composition analysis revealed significant variations among Bombyx mori varieties, with sericin from different sources containing high levels of L-serine, suggesting diverse bioactive potential. The role of D-serine, a derivative of L-serine, in osteoclast differentiation was examined using RAW264.7 cells. Pretreatment with D-serine significantly suppressed the expression of tartrate-resistant acid phosphatase (TRAP) induced by RANKL and M-CSF, indicating its inhibitory effect on osteoclast activity. Serum analysis following sericin administration in healthy rats demonstrated that a single dose of sericin (25 mg/kg) significantly increased serum D-serine levels compared to the control group (p < 0.001). Although no significant differences were observed among groups fed with varying concentrations of sericin over three days, higher baseline serum D-serine levels were noted in the higher-dose groups. These findings highlight sericin's potential as a functional food component for bone health, driven by its high L-serine content and the osteoclast-inhibitory effects of D-serine.