구강내 임플랜트-지대주 연결부에서 치주질환관련 세균의 검출
Detection of periodontal disease related bacteria from the implant-abutment interface in oral cavity
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
문제 진술: 임플랜트 시스템은 임플랜트와 지대주 사이에서 빈 공간이 발생하여 세균의 저장소로서 작용하게 되어 임플랜트 주위 연조직 에 염증반응을 야기할 수 있다. 목 적: 이 연구의 목적은 임플랜트-지대주 간극의 미세 누출에 관여하는 치주질환원인균으로 추정되는 Porpyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans의 검출률을 조 사하는 것이다. 연구재료 및 방법: 시료채취는 27명의 환자들에서 행하였고 소독된 페이퍼포인트를 이용하여 1×PBS로 옮겼다. 치주질환원인균의 검출 은 16 rDNA에 근거한 종특이적인 프라이머를 지닌 중합효소체인반응을 이용하였다. 결 과: 임플랜트 고정체내에서의 Porphyromonas gingivalis와 Prevotella intermedia의 검출률은 59%와 82%였다. 환자의 임플 랜트 연구에서는 Porphyromonas gingivalis와 Prevotella intermedia의 검출률은 44%와 82%였다. 환자혀에서의 Porphyromonas gingivalis와 Prevotella intermedia의 검출률은 82%와 82%였다. 결 론: 현재의 임플랜트 시스템은 임플랜트 내부의 세균 군집화와 미세누출을 안전하게 예방할 수 없다.
Abstract
Statement of the problem: Implant systems result in gaps and cavities between implant and abutment that can act as a trap for bacteria and thus possibly cause inflammatory reactions in the peri-implant soft tissues. Purpose: Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, and Aggregatibacter actinomycetemcomitans, related to implant-abutment interface microleakage. Material and methods: Samples were taken from 27 subjects with sterilized paper points and were transported in 1×PBS. The detection of periodontopathogens were performed by polymerase chain reaction with species-specific primers based on 16S rDNA. Results: Our data showed that the detection rate of P. gingivalis and P. intermedia in implant fixture was 59% and 82% in patients respectively. Detection rate of P. gingivalis and P. intermedia in implant crevice was 44% and 82% in patients. Detection rate of P. gingivalis and P. intermedias in tongue was 82% and 82% in patients. Conclusion: Current implant systems cannot safely prevent microbial leakage and bacterial colonization of the inner part of the implant.
참고문헌 (27)
- Volker K, "Microbial leakage and marginal fit of the implant-abutment interface", Int J Oral Maxillofac Implants, 12, pp.527~540 (1997)
- Steinebrunner L, "In vitro Evaluation of Bacterial Leakage Along the Implant-Abutment Interface of Different Implant Systems", Int J Oral Maxillofac Implants, 20, pp.875~881 (2005)
- Gross M, "Microleakage at the abutment-implant interface of osseointegrated implants: a comparative study", Int J Oral Maxillofac Implants, 14, pp.94~100 (1999)
- Quirynen M, "Microbial penetration along the implant components of the Branemark system. An in vitro study", Clin Oral Impl Res, 5, pp.239~244 (1994)
- Abrahamsson I, "Soft tissue response to plaque formation at different implant systems. A comparative study in the dog", Clin Oral Impl Res, 9, pp.73~79 (1998)
- Mombelli A, "The microbiota associated with successful or failing osseointegrated titanium im-plants", Oral Microbiology and Immunology, 2, pp.145~151 (1987)
- Broggini N, "Persistent acute inflammation at the implant-abutment interface", J Dent Res, 82, pp.232~237 (2003)
- Binon PP, "The effect of implant", abutment hexagonal misfit on screw joint stability, 1996, pp.9, 149~160
- Quirynen M, "Bacterial colonisation of the interanl part of two-stage implants. An in vivo study", Clinial Oral Implants Research, 4, pp.158~161 (1993)
- Lim HH, "Frequency of species and biotypes of mutans streptococci isolated from dental plaque in the adolescents and adults", J Bacteriol Virol, 35, pp.197~201 (2005)
- Lee SE, "Direct identification of Vibrio vulnificus in clinical specimens by nested PCR", J Clin Microbiol, 36, pp.2887~2892 (1998)
- Ashmito A, "Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions", Oral Microbiology and Immunology, 11, pp.266~273 (1996)
- Stubbs S, "Direct detection of Prevotella intermedia and P. nigrescens in suppurative oral infection by amplification of 16S rRNA gene", J Med Microbiol, 48, pp.1017~1022 (1999)
- Grossi SG, "Assessment of risk for periodontal disease II Risk indicators for alveolar bone loss", J Periodontol, 66, pp.23~29 (1995)
- Grossi SG, "of risk for periodontal disease. I. Risk indicators for attachment loss", J Periodontol, 65, pp.260~267 (1994)
- Ximenez-Fyvie LA, "Comparison of the microbiota of supra-and subgingival plaque in health and periodontitis", J Clin Periodontol, 27, pp.648~657 (2000)
- Gersdorf H, "Identification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis", J Clin Microbiol, 31, pp.941~946 (1993)
- Holt SC, "Factors in virulence expression and their role in periodontal disease pathogenesis", Crit Rev Oral Biol, 2, pp.177~281 (1991)
- Saito T, "Cloning, expression and sequencing of a protease gene from Bacteroides forsythus ATCC 43037 in Escherichia coli", Infect Immun, 65, pp.4888~4891 (1997)
- Okamato M, "Hemolytic and hemagglutinating activities of Prevotella intermedia and Prevotella nigrescens", FEMS Microbiol Lett, 178, pp.299~304 (1999)
- Leung KP, "Effects of porphyrins and inorganic ion on the growth of Prevotella intermedia", FEMS Microbiol Lett, 209, pp.15~21 (2002)
- Hakkarainen K, "A 7-month study of sulcular fluid flow in the assessment of healing after debridement of deep pockets", J Periodontol, 57, pp.14~19 (1986)
- Lang NP, "Bleedin on probing. A predictor for the progression of periodontal disease?", J Clin Periodontol, pp.590~586 (1986)
- Kook JK, "Detection of Tannerella forsythia and", or Prevotella intermedia Might be useful for microbial predictive markers fot the outcome of initial periodontal treatment in Koreans, 2005, pp.49, 9~16
- Jansen VK, "Microbial leakage and marginal fit of the implant-abutment interface", Int J Oral Maxillofac Implants, 12, pp.527~540 (1997)
- Dixon DL, "Comparison of screw loosening, rotation, and deflection among three implant designs", J Prosthet Dent, 74, pp.270~278 (1995)
- Besimo D, "Marginal fit of prefabircated crowns of the Ha-Ti implant system:An in vitro scanning electron microscopic study", Int J Prosthodont, 9, pp.87~94 (1996)