Machined 티타늄 임플란트와 calcium phosphate coated 티타늄 임플란트의 조직형태계측학적 연구
Histomorphometric study of machined titanium implants and calcium phosphate coated titanium implants
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연구목적: 본 연구는 machined 임플란트와 골유도능이 있는 calcium phosphate를 electron-beam deposition으로 coating처리한 임플란트의 골/임플란트 접촉률을 조직형태계측학적으로 비교하는 것이다. 연구 재료 및 방법: 여섯 마리의 수컷 New Zealand white rabbit과 직경 3.3 mm, 길이 5 mm의 임플란트 24개를 준비하였다. Machined 임플란트 (대조군)와 calcium phosphate coated 임플란트 (실험군)를 좌, 우 경골에 2개씩 총 4개를 식립하고 임플란트 주위에 부하가 가해지지 않도록 하여 3주, 6주의 치유기간을 두었다. 식립 3주와 6주후, 각각 3마리의 토끼를 희생하여 조직시편을 제작하였다. 제작된 시편을 광학현미경 하에서 골/임플란트 접촉률 (BIC ratio)을 계산하고 paired t-test로 두 군을 비교하였다. 결과: 골/임플란트 접촉률은 임플란트 식립 3주후, 대조군에서 평균과 표준편차는 44.1 ± 16.5% 이었고 실험군은 70.8 ± 18.9%로 실험군이 통계적으로 유의하게 높았다 (P = 0.0264). 6주후의 경우, machined 임플란트는 78.6 ± 15.1%, calcium phosphate coated 임플란트는 79.0 ± 26.0%로 두 군 간 통계적으로 유의한 차이는 없었다. 결론: Calcium phosphate coated 임플란트는 machined 티타늄 임플란트에 비해 빠른 초기 골반응을 나타냈다. 그러므로, 임상적으로 calcium phosphate coated 임플란트를 사용했을때, 수술 후 치유 기간을 단축하여 조기 부하가 가능할 것으로 사료된다.
Abstract
Purpose: The objective of this study was to investigate the effects of calcium phosphate coated titanium implant surface on bone response and implant stability at early stage of healing period of 3 weeks and later healing period of 6 weeks. Material and methods: A total of 24 machined, screw-shaped implants (Dentium Co., Ltd., Seoul, Korea) which dimensions were 3.3 mm in diameter and 5.0 mm in length, were used in this research. All implants (n = 24), made of commercially pure (grade IV) titanium, were divided into 2 groups. Twelve implants (n = 12) were machined without any surface modification (control). The test implants (n = 12) were anodized and coated with thin film (150 nm) of calcium phosphate by electron-beam deposition. The implants were placed on the proximal surface of the rabbit tibiae. The bone to implant contact (BIC) ratios was evaluated after 3 and 6 weeks of implant insertion. Results: The BIC percentage of calcium phosphate coated implants (70.8 ± 18.9%) was significantly higher than that of machined implants (44.1 ± 16.5%) 3 weeks after implant insertion (P = 0.0264). However, there was no significant difference between the groups after 6 weeks of healing (P > .05). Conclusion: The histomorphometric evaluation of implant surface revealed that: 1. After 3 weeks early healing period, bone to implant contact (BIC) percentage of calcium phosphate coated implants (70.8%) was much greater than that of surface untreated machined implants (44.1%) with P = 0.0264. 2. After 6 weeks healing period, however, BIC percentage of calcium phosphate coated implants group (79.0%) was similar to the machined only implant group (78.6%). There was no statistical difference between two groups (P = 0.8074). 3. We found the significant deference between the control group and experimental group during the early healing period of 3 weeks. But no statistical difference was found between two groups during the later of 6 weeks.
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