지르코니아의 생체적합성에 대한 연구: In vitro 실험 문헌 고찰
A review of biocompatibility of zirconia: In vitro experiment
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
진료실에서 지르코니아의 수요가 증가하고 있음에 따라 지르코니아의 생체적합성에 대한 평가가 필수적이다. 이번 논문에서는 지르코니아의 생체적 합성에 대한 문헌 중 in vitro 실험에서의 결과를 고찰하였다. Fibroblast, osteoblast, lymphocyte 등 다양한 세포를 이용한 in vitro 실험에서 지르코니아 블록은 뛰어난 생체적합성을 보였다. 여러 연구에서 지르코니아에 대한 세포독성 및 돌연변이 유발이 관찰되지 않았으며, 세균부착도 적은 것으로 나타났다. 장기간 체액노출조건을 모방한 in vitro 실험에서는 약간의 기계적 강도의 감소 외에는 악영향이 관찰되지 않았다. Osteoblast-like cell을 이용한 연구에서, 지르코니아 블록이 면역반응, 물질이동, 세포주기조절에 관여하는 유전자를 조절하여 생체적합을 높이는 것으로 관찰되었다. 여러 in vitro 연구에서 지르코니아 분말의 생체적합성에 대해서는 상이한 보고가 나타나고 있다. 종합적으로 지르코니아 분말은 어느 정도 세포독성을 가지고 있는 것으로 생각된다.
Abstract
Increasing demands for zirconia material in clinics, assessment of biocompatibility of zirconia is essential. In this article, a review of in vitro studies of zirconia compatibility was performed. Zirconia showed great biocompatibility at in vitro studies with various cell lines such as fibroblasts, osteoblasts, and lymphocytes. Many studies reported that zirconia caused no cytotoxicity or mutation. Zirconia also showed less bacterial adhesion. There were no adverse effects except for small reduced strength with in vitro study mimicking long-term exposure of body fluid. According to the study with ostoblast-like cells, zirconia could regulate genes of immunity, molecular transport, and cell cycle. Such gene regulating was considered as one of the reasons of zirconia biocompatibility. With biocompatibility of zirconia powders, in vitro studies had controversial conclusions. It seems that zirconia powders might have cytotoxicity.
참고문헌 (18)
- Kosmac T, "The effect of surface grinding and sandblasting on flexural strength and reliability of Y-TZP zirconia ceramic", Dent Mater, 15, pp.426~433 (1999)[DOI]
- Al-Amleh B, "Clinical trials in zirconia: a systematic review", J Oral Rehabil, 37, pp.641~652 (2010)
- Manicone PF, "Biological considerations on the use of zirconia for dental devices", Int J Immunopathol Pharmacol, 20, pp.9~12 (2007)
- Josset Y, "In vitro reactions of human osteoblasts in culture with zirconia and alumina ceramics", J Biomed Mater Res, 47, pp.481~493 (1999)[DOI]
- Fassina P, "Bioceramics and the human body", pp.223~229 (1992), Springer
- Silva VV, "Biological reactivity of zirconia-hydroxyapatite composites", J Biomed Mater Res, 63, pp.583~590 (2002)[DOI]
- Carinci F, "Zirconium oxide: analysis of MG63 osteoblastlike cell response by means of a microarray technology", Biomaterials, 25, pp.215~228 (2004)[DOI]
- Garvie RC, "Biocompatibility of Magnesia Partially Stabilized Zirconia (Mg-PSZ) Ceramics", J Mater Sci, 19, pp.3224~3228 (1984)[DOI]
- Rimondini L, "Bacterial colonization of zirconia ceramic surfaces: an in vitro and in vivo study", Int J Oral Maxillofac Implants, 17, pp.793~798 (2002)
- Covacci V, "In vitro evaluation of the mutagenic and carcinogenic power of high purity zirconia ceramic", Biomaterials, 20, pp.371~376 (1999)[DOI]
- Ito A, "In-vitro evaluation of the cytocompatibility of wear particles generated by UHMWPE", zirconia friction, 1993, pp.12, 203~209
- Catelas I, "Induction of macrophage apoptosis by ceramic and polyethylene particles in vitro", Biomaterials, 20, pp.625~630 (1999)[DOI]
- Li J, "Evaluation of biocompatibility of various ceramic powders with human fibroblasts in vitro", Clin Mater, 12, pp.197~201 (1993)[DOI]
- Nkamgueu EM, "In vitro effects of zirconia and alumina particles on human blood monocyte-derived macrophages: X-ray microanalysis and flow cytometric studies", J Biomed Mater Res, 52, pp.587~594 (2000)[DOI]
- Dion I, "Physico-chemistry and cytotoxicity of ceramics: part I: characterization of ceramic powders", J Mater Sci Mater Med, 8, pp.325~332 (1997)[DOI]
- Lohmann CH, "Ceramic and PMMA particles differentially affect osteoblast phenotype", Biomaterials, 23, pp.1855~1863 (2002)[DOI]
- Sterner T, "Effects of clinically relevant alumina ceramic, zirconia ceramic and titanium particles of different sizes and concentrations on TNFalpha release in a human macrophage cell line", Biomed Tech (Berl), 49, pp.340~344 (2004)
- Tateishi T, "Round robin test for standardization of biocompatibility test procedure by cell culture method", Adv Biomater, 10, pp.89~97 (1992)