자작나무와 오리나무 화분의 주 알레르겐에 대한 immunoglobulin E 결합 성분의 규명
Identification of immunoglobulin E binding components of two major tree pollens, birch and alder
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Purpose: Pollinosis is one of the major allergic diseases caused by airborne pollens. Alder and birch pollens are the major sensitizing tree pollens in this country. The immunoglobulin E (IgE) reactivity to each pollen allergen is known to be variable according to the region. We determined the major IgE binding components of these tree pollens in sera of adult patients with allergic rhinitis. Methods: Allergic rhinitis patients, of whom specific IgE level to birch and/or alder pollens (>10 kU/L by ImmunoCAP) were included. The protein bands of two pollen extracts were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and their IgE-binding components were identified by IgE immunoblot analysis. The binding specificity and cross-reactivity between two pollens were evaluated by IgE enzyme linked immunosorbent assay (ELISA) inhibition test. Results: Six IgE binding components were found in birch pollens in which two (14 kDa and 17 kDa) were major components. Two IgE binding components were found in alder pollens in which the 17 kDa was a major component. The IgE binding component to the major allergen component of 17 kDa was observed in 90.3% of the study subjects sensitive to alder pollens and 72.7% of them sensitive to birch pollens. The ELISA inhibition tests showed significant inhibitions with additions of birch/alder pollen extracts. Conclusion: We identified two major IgE binding components (17 kDa and 14 kDa) from birch pollens and one component (17 kDa) from alder pollens. Significant cross reactivity was noted between these two pollens.
Abstract
Purpose: Pollinosis is one of the major allergic diseases caused by airborne pollens. Alder and birch pollens are the major sensitizing tree pollens in this country. The immunoglobulin E (IgE) reactivity to each pollen allergen is known to be variable according to the region. We determined the major IgE binding components of these tree pollens in sera of adult patients with allergic rhinitis. Methods: Allergic rhinitis patients, of whom specific IgE level to birch and/or alder pollens (>10 kU/L by ImmunoCAP) were included. The protein bands of two pollen extracts were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and their IgE-binding components were identified by IgE immunoblot analysis. The binding specificity and cross-reactivity between two pollens were evaluated by IgE enzyme linked immunosorbent assay (ELISA) inhibition test. Results: Six IgE binding components were found in birch pollens in which two (14 kDa and 17 kDa) were major components. Two IgE binding components were found in alder pollens in which the 17 kDa was a major component. The IgE binding component to the major allergen component of 17 kDa was observed in 90.3% of the study subjects sensitive to alder pollens and 72.7% of them sensitive to birch pollens. The ELISA inhibition tests showed significant inhibitions with additions of birch/alder pollen extracts. Conclusion: We identified two major IgE binding components (17 kDa and 14 kDa) from birch pollens and one component (17 kDa) from alder pollens. Significant cross reactivity was noted between these two pollens.
참고문헌 (19)
- 오재원, "The Revised Edition of Korean Calendar for Allergenic Pollens", Allergy, Asthma & Immunology Research, 4(1), pp.5~11 (2012), 대한천식알레르기학회
- "The Angiosperm Phylogeny Group. An update of the angiosperm phylogeny group classification for the orders and families of flowering plants: APG II", Bot J Linn Soc, 141, pp.399~436 (2003)
- Niederberger V, "Recombinant birch pollen allergens (rBet v 1 and rBet v 2) contain most of the IgE epitopes present in birch, alder, hornbeam, hazel, and oak pollen: a quantitative IgE inhibition study with sera from different populations", J Allergy Clin Immunol, 102, pp.579~591 (1998)
- Park HS, "Survey of airborne pollens in Seoul, Korea", J Korean Med Sci, 9, pp.42~46 (1994)
- 이진우, "1999년, 2005년, 2008년 피부반응검사 분석을 통해 본 지난 10년간 경기 남부지역 대학병원에 내원한 알레르기 환자의 화분 알레르겐 감작률의 변화", 천식 및 알레르기, 31(1), pp.33~40 (2011), 대한천식알레르기학회
- Oh JW, "Aerobiological study of pollen and mold in Seoul, Korea", Allergol Int, 47, pp.263~270 (1998)
- 오재원, "대기 중 알레르기 화분(꽃가루) 농도 예보제 개발", 대한의사협회지, 52(6), pp.579~591 (2009), 대한의사협회
- Movérare R, "Different IgE reactivity profiles in birch pollen-sensitive patients from six European populations revealed by recombinant allergens: an imprint of local sensitization", Int Arch Allergy Immunol, 128, pp.325~335 (2002)
- Ebner C, "Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin)", J Allergy Clin Immunol, 95(5 Pt 1), pp.962~969 (1995)
- 김경호, "부산지역 호흡기 알레르기 환자에서 흡입 알레르겐에 대한 감작률", 천식 및 알레르기, 25(1), pp.59~63 (2005), 대한천식알레르기학회
- Smith M, "Regional importance of Alnus pollen as an aeroallergen: a comparative study of Alnus pollen counts from Worcester (UK) and Poznań (Poland)", Ann Agric Environ Med, 14, pp.123~128 (2007)
- Pauli G, "Skin testing with recombinant allergens rBet v 1 and birch profilin, rBet v 2: diagnostic value for birch pollen and associated allergies", J Allergy Clin Immunol, 97, pp.1100~1109 (1996)
- Jarolim E, "IgE and IgG antibodies of patients with allergy to birch pollen as tools to define the allergen profile of Betula verrucosa", Allergy, 44, pp.385~395 (1989)
- Valenta R, "Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals", Science, 253, pp.557~560 (1991)
- Valenta R, "Profilins constitute a novel family of functional plant pan-allergens", J Exp Med, 175, pp.377~385 (1992)
- van Ree R, "Profilin is a cross-reactive allergen in pollen and vegetable foods", Int Arch Allergy Immunol, 98, pp.97~104 (1992)
- Huh JE, "Protective effects of butanol fraction from Betula platyphyla var. japonica on cartilage alterations in a rabbit collagenase-induced osteoarthritis", J Ethnopharmacol, 123, pp.515~521 (2009)
- Park JW, "Cross - reactivity between pollens in patients sensitlzed to multiple pollens", J Asthma Allergy Clin Immunol, 19, pp.584~593 (1999)
- Mothes N, "Transition from a botanical to a molecular classification in tree pollen allergy: implications for diagnosis and therapy", Int Arch Allergy Immunol, 135, pp.357~373 (2004)