돼지풀과 쑥 화분의 주 알레르겐에 대한 IgE 결합 성분의 규명
Identification of IgE binding components of two major weed pollens, ragweed and mugwort
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Purpose: Ragweed and mugwort pollens are the major weed allergens that cause pollinosis in Korea. The IgE-binding components to these 2 pollens and their cross-reactivity have not been reported in Korea, while several reports had been made in Western countries. We investigated IgE-binding components to ragweed and mugwort pollens and their allergenic relationship in patients sensitive to the 2 pollens. Methods: We enrolled 33 allergic rhinitis patients with typical seasonal pollinosis symptoms in autumn and elevated serum specific IgE levels to ragweed and/or mugwort pollens (>10 kU/L by ImmunoCAP). The protein bands of the 2 pollen extracts were determined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and IgE immunoblot analysis was performed to determine the IgE-binding components of each pollen extract. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot inhibition tests were performed to evaluate the cross-reactivity between ragweed and mugwort pollen extracts. Results: Eight IgE-binding components (9, 10, 11, 12, 27, 30, 38, and 80 kDa) were found in ragweed pollen extracts, of which 4 (38, 11, 27, and 80 kDa) were major IgE-binding components. Eight IgE-binding components (10, 14, 16, 20–24, 26–30, 42, 60–66, and 80–90 kDa) were found in mugwort pollen extracts, of which 2 components (26–30 and 20–24 kDa) were major IgE-binding components. No significant inhibitions were noted between ragweed and mugwort pollen extracts by the ELISA inhibition test. No significant changes were noted in IgE immunoblot inhibition analysis. Conclusion: We identified 4 major IgE-binding components (38, 11, 35, 27, and 80 kDa) in ragweed pollens and 2 major IgE-binding components (26–30 and 20–24 kDa) in mugwort pollens. No cross-reactivity was found between ragweed and mugwort pollens.
Abstract
Purpose: Ragweed and mugwort pollens are the major weed allergens that cause pollinosis in Korea. The IgE-binding components to these 2 pollens and their cross-reactivity have not been reported in Korea, while several reports had been made in Western countries. We investigated IgE-binding components to ragweed and mugwort pollens and their allergenic relationship in patients sensitive to the 2 pollens. Methods: We enrolled 33 allergic rhinitis patients with typical seasonal pollinosis symptoms in autumn and elevated serum specific IgE levels to ragweed and/or mugwort pollens (>10 kU/L by ImmunoCAP). The protein bands of the 2 pollen extracts were determined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and IgE immunoblot analysis was performed to determine the IgE-binding components of each pollen extract. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot inhibition tests were performed to evaluate the cross-reactivity between ragweed and mugwort pollen extracts. Results: Eight IgE-binding components (9, 10, 11, 12, 27, 30, 38, and 80 kDa) were found in ragweed pollen extracts, of which 4 (38, 11, 27, and 80 kDa) were major IgE-binding components. Eight IgE-binding components (10, 14, 16, 20–24, 26–30, 42, 60–66, and 80–90 kDa) were found in mugwort pollen extracts, of which 2 components (26–30 and 20–24 kDa) were major IgE-binding components. No significant inhibitions were noted between ragweed and mugwort pollen extracts by the ELISA inhibition test. No significant changes were noted in IgE immunoblot inhibition analysis. Conclusion: We identified 4 major IgE-binding components (38, 11, 35, 27, and 80 kDa) in ragweed pollens and 2 major IgE-binding components (26–30 and 20–24 kDa) in mugwort pollens. No cross-reactivity was found between ragweed and mugwort pollens.
참고문헌 (25)
- 이진우, "1999년, 2005년, 2008년 피부반응검사 분석을 통해 본 지난 10년간 경기 남부지역 대학병원에 내원한 알레르기 환자의 화분 알레르겐 감작률의 변화", 천식 및 알레르기, 31(1), pp.33~40 (2011), 대한천식알레르기학회
- 오재원, "The Revised Edition of Korean Calendar for Allergenic Pollens", Allergy, Asthma & Immunology Research, 4(1), pp.5~11 (2012), 대한천식알레르기학회
- 오재원, "대기 중 알레르기 화분(꽃가루) 농도 예보제 개발", 대한의사협회지, 52(6), pp.579~591 (2009), 대한의사협회
- 오재원, "소아 알레르기 유발 가능성과 화분(꽃가루)농도와의 관련성", Allergy Asthma & Respiratory Diseases, 19(1), pp.4~11 (2009), 대한 소아알레르기 호흡기학회
- So Hyun Park, "Sensitization rates of airborne pollen and mold in children", Korean Journal of Pediatrics, 55(9), pp.372~379 (2012), 대한소아과학회
- 이지은, "Variability of Offending Allergens of Allergic Rhinitis According to Age: Optimization of Skin Prick Test Allergens", Allergy, Asthma & Immunology Research, 6(1), pp.47~54 (2014), 대한천식알레르기학회
- Wopfner N, "The spectrum of allergens in ragweed and mugwort pollen", Int Arch Allergy Immunol, 138, pp.337~346 (2005)
- 김주화, "Changes in Sensitization Rate to Weed Allergens in Children with Increased Weeds Pollen Counts in Seoul Metropolitan Area", Journal of Korean Medical Science, 27(4), pp.350~355 (2012), 대한의학회[DOI]
- 오재원, "한국 공중화분의 특징과 최근의 변화", 천식 및 알레르기, 27(1), pp.1~7 (2007), 대한천식알레르기학회
- Oberhuber C, "Prevalence of IgE-binding to Art v 1, Art v 4 and Amb a 1 in mugwort-allergic patients", Int Arch Allergy Immunol, 145, pp.94~101 (2008)
- Moverare R, "Mugwort-sensitized individuals from North Europe, South Europe and North America show different IgE reactivity patterns", Int Arch Allergy Immunol, 154, pp.164~172 (2011)
- Han D, "The specific IgE reactivity pattern of weed pollen-induced allergic rhinitis patients", Acta Otolaryngol, 131, pp.533~538 (2011)
- Katial RK, "Mugwort and sage (Artemisia) pollen cross-reactivity: ELISA inhibition and immunoblot evaluation", Ann Allergy Asthma Immunol, 79, pp.V340~V346 (1997)
- Hirschwehr R, "Identification of common allergenic structures in mugwort and ragweed pollen", J Allergy Clin Immunol, 101(2 Pt 1), pp.196~206 (1998)
- Weber RW, "Patterns of pollen cross-allergenicity", J Allergy Clin Immunol, 112, pp.229~239 (2003)
- Park HS, "Antigenic relationship between mugwort and ragweed pollens by crossed immunoelectrophoresis", J Korean Med Sci, 9, pp.213~217 (1994)
- Asero R, "Artemisia and Ambrosia hypersensitivity: co-sensitization or co-recognition?", Clin Exp Allergy, 36, pp.658~665 (2006)
- 윤문경, "자작나무와 오리나무 화분의 주 알레르겐에 대한 immunoglobulin E 결합 성분의 규명", Allergy Asthma & Respiratory Diseases, 1(3), pp.216~220 (2013), 대한 소아알레르기 호흡기학회
- Hao GD, "Prevalence of sensitization to weed pollens of Humulus scandens, Artemisia vulgaris, and Ambrosia artemisiifolia in northern China", J Zhejiang Univ Sci B, 14, pp.240~246 (2013)
- Jahn-Schmid B, "Humoral and cellular cross-reactivity between Amb a 1, the major ragweed pollen allergen, and its mugwort homolog Art v 6", J Immunol, 188, pp.1559~1567 (2012)
- Jahn-Schmid B, "The T cell response to Art v 1, the major mugwort pollen allergen, is dominated by one epitope", J Immunol, 169, pp.6005~6011 (2002)
- Jimeno L, "Monoclonal antibody-based ELISA to quantify the major allergen of Artemisia vulgaris pollen, Art v 1", Allergy, 59, pp.995~1001 (2004)
- Himly M, "Art v 1, the major allergen of mugwort pollen, is a modular glycoprotein with a defensin-like and a hydroxyproline-rich domain", FASEB J, 17, pp.106~108 (2003)
- Canis M, "IgE reactivity patterns in patients with allergic rhinoconjunctivitis to ragweed and mugwort pollens", Am J Rhinol Allergy, 26, pp.31~35 (2012)
- Gadermaier G, "Array-based profiling of ragweed and mugwort pollen allergens", Allergy, 63, pp.1543~1549 (2008)