Tertiary Structure of PreS1(21-47) of Hepatitis B Virus Studied by NMR Spectroscopy

Abstract

To design more efficient peptide antagonist against the HBV, preS1(21-47) which carries the HBV receptor binding site for hepatocytes was synthesized and the solution structure of preS1(21-47) was investigated using CD spectroscopy and NMR spectroscopy in membrane-mimicking environments. According to CD spectra, preS1(21-47) has a random structure in aqueous solution, while conformational change was induced by addition of TFE and SDS micelle. Tertiary structure as determined by NMR spectroscopy shows that preS1(21-47) has a very flexible structure even in SDS micelle.