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Conformational study of decamer DNA duplex d(ACGTATACGT)2 by NMR spectroscopy

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Abstract

The conformation of the self-complementary decamer duplex, d(ACGTATACGT)2 (TATA-duplex) has been studied by proton NMR spectroscopy. The duplex is essentially B-type, with distortions apparent at the TATA steps. These conformational distortion which may be preferable to occur in the thymine residue on the 5-side, has been investigated by unusual NOE crosspeaks.

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