본문 바로가기
KERD
논문KCI 등재

當歸 香氣液이 Rat의 뇌신경줄기세포의 分化와 增殖에 미치는 영향

The Effect of an Essential Oil Fragrance from Radix Angelica Sinesis on Differentiation and Proliferation of Neural Stem Cells of Rat

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류한의학

초록

Objectives : In this study, an essential oil fragrance from Danggwi was administrated into the neural stem cell and the effect of the essential oil on the differentiation and proliferation of the neural stem cells were observed. Methods : The establishment of the neural stem cell was identified via Nestin, DAPI dye. An essential oil fragrance from Danggwi was administrated with a proved optimum level for the survival of the cell through MTT assay. Also, according to the analysis of Western blot, the essential oil fragrance from Danggwi promotes the phosphorylating of Akt, Erk, ERM protein. Results : MTT assay showed increased in GFAP. The result indicates that the differentiation to astrocyte is promoted. The phosphorylation levels of ERM, Erk and Akt were increased at 60 min after addition of 5 ug/ml of essential oil fragrance from Danggwi and sustained to 48 hours. These imply that essential oil fragrance from Danggwi may induce the survival and the proliferation of the differentiated cells. Conclusions : These results suggest that the essential oil fragrance from Danggwi can be effective for the in vivo study of degenerative neuronal disease using neural stem cell.

Abstract

Objectives : In this study, an essential oil fragrance from Danggwi was administrated into the neural stem cell and the effect of the essential oil on the differentiation and proliferation of the neural stem cells were observed. Methods : The establishment of the neural stem cell was identified via Nestin, DAPI dye. An essential oil fragrance from Danggwi was administrated with a proved optimum level for the survival of the cell through MTT assay. Also, according to the analysis of Western blot, the essential oil fragrance from Danggwi promotes the phosphorylating of Akt, Erk, ERM protein. Results : MTT assay showed increased in GFAP. The result indicates that the differentiation to astrocyte is promoted. The phosphorylation levels of ERM, Erk and Akt were increased at 60 min after addition of 5 ug/ml of essential oil fragrance from Danggwi and sustained to 48 hours. These imply that essential oil fragrance from Danggwi may induce the survival and the proliferation of the differentiated cells. Conclusions : These results suggest that the essential oil fragrance from Danggwi can be effective for the in vivo study of degenerative neuronal disease using neural stem cell.

참고문헌 (76)

  1. 서광식, "줄기세포란 무엇인가?", 경희의학, 21(2), pp.110~116 (2005)
  2. 최욱환, "줄기세포 치료의 연구 현황 및 최신 지견", Korean Journal of Obstetrics and Gynecology, 50(4), pp.569~579 (2007), 대한산부인과학회
  3. RietzeRI, "Purication ofapluripotent neuralstem cellfrom the adultmousebrain", Nature, 412, pp.736~739 (2001)
  4. KrugerGM, "Neural creststem cellpersisin the adultgut but undergo changes in self-renewal, neuronalsubtype potential,and factor responssiveness", Neuron, 35, pp.657~669 (2002)
  5. RidetJL, "Reactiveastrcytes:cellularand molecular cuestobiologicalfunction", TrendsNeurosci, 20, pp.570~577 (1997)
  6. TacconiMT, "Neuroaldeath :istherea roleforastrcytes?", Neurochem Res, 23, pp.759~765 (1998)
  7. RobinsDS, "Production of cytotoxicfactorforoligodendrocytesby stimulatedastrcytes", JImmunol, 139, pp.2593~2597 (1987)
  8. LevineJB, "Astrocytes interact intimately with degenerating motor neurons in mouse amyotrophic latralsclerosis(ALS)", Glia, 28, pp.215~224 (1999)
  9. PeknyM, "Abnormal reaction to central nervous system injury in mice lacking glial fibrillary acidicprotein and vimentin", J CellBiol, 145, pp.503~514 (1999)
  10. TaoR, "Glialcellresponse,complement and apolipoprotein J expressionfollowingaxoninjuryinthe neonatalrat", JNeurocytol, 28, pp.559~570 (1999)
  11. RaivichG, "Molecular signals for glialactivation:pro-and anti-inflammatory cytokinesin theinjuredbrain", ActaNeurochirSuppl, 73, pp.21~31 (1999)
  12. Buckle J, "Use ofaromatherapy as a complementary treatment for chronic pain", Alternative Therapies in Health& Medicine, 5(5), pp.42~51 (1999)
  13. Wheeler Robins JL, "The science and artofaromatherapy", JournalofHolistic Nursing, 17(1), pp.5~17 (1999)
  14. "靈樞經校釋", pp.289~290 (1982), 人民衛生出版社
  15. 任應秋, "黃帝內經章句索引", pp.17~ (1986), 人民衛生出版社
  16. 김준희, "아로마 선호에 관한 연구", 한국주관성연구학회, 8, pp.158~161 (2003)
  17. 김종철, "일차 의료인을 위한 아로마테라피의 소개", 가정의학회지, 23(4), pp.423~425 (2002)
  18. 全國韓醫科大學本草學敎授, "本草學", pp.578~580 (1991), 永林社
  19. 임종국, "당귀약침액의 항산화 효능에 관한 연구(2)", 대한침구학회지, 14(1), pp.383~396 (1997)
  20. 전연이, "An Experimental Study of Effect on Brain Damage and Neuroprotective Effect of Angelicae Gigantis Radix extract against Cerebral Ischemia in Rats", 대한본초학회지, 18(4), pp.25~35 (2003), 대한본초학회
  21. 張均田, "人蔘皂苷Rg1的促智作用機制對神經可望性和神經發生的影響", 藥學學報, 40(5), pp.385~388 (2005)
  22. 單德紅, "定志小丸對抑鬱模型大鼠齒狀回神經干細胞和學習記憶能力的影響", 中醫藥學刊, 23(8), pp.1426~1427 (2005)
  23. 繆兵, "銀杏內酯B對神經細胞活性影響的實驗硏究", 山東醫藥, 44(20), pp.16~18 (2004)
  24. 司銀楚, "三七總皂苷促進離體胎鼠皮層神經干細胞增殖,分化的實驗硏究", 中國體視學與圖像分析, 9(2), pp.78~83 (2004)
  25. 劉柏炎, "補陽還五湯對低溏低氧損傷神經干細胞移行分化的影響", 中國藥物與臨床, 5(3), pp.171~173 (2005)
  26. IsacsonO, "Neuraltransplantation studies frvea the brain’s capacity for continuousreconstruction", TrendsNeurosci, 20, pp.477~482 (1997)
  27. DahlstrandJ, "Nestin mRNA expression correlates with the centralnervoussystem progenitorcell state in many,butnotall,regionsof developingcentralnervoussystem", Brain resDevBrainRes, 84, pp.109~129 (1995)
  28. 이광호, "백서에서 척수의 허혈", 재관류 손상시 neuronal Nitric Oxide Synthase(nNOS)의 발현, 2007, pp.52(4), 449~453[DOI]
  29. Sladowski.D, "An improved MTT assay", J. Immunol.Methods, 157, pp.203~207 (1993)
  30. Schraen-MaschkeS, "2004 Microtubuleassociatedproteintaugene:ariskfactor in human neurodegenerative diseases", NeurobiolDis, 15(3), pp.449~460 (2004)
  31. NonakaJ, "CoCl(2)inhibitsneuraldifferentiation ofretinoicacid-treatedembryoidbodies", JBiosciBioeng, 106(2), pp.141~147 (2008)
  32. DattaSR, "Greenberg ME.Akt phosphorylationofBAD couplessurvival signalstothecell-intrinsicdeathmachinery", Cell, 91, pp.231~241 (1997)
  33. Cardone MH, "Regulation ofcell deathproteasecaspase-9byphosphorylation", Science, 282, pp.1318~1321 (1998)
  34. Sebolt-LeopoldJS, "Targeting themitogen-activatedproteinkinasecascade totreatcancer", NatRevCancer, 4, pp.937~947 (2004)
  35. Tsukita S, "Corticalactin organization :lesson from ERM(ezrin", radixin, JBiolChem, pp.1999, 9(21)[DOI]
  36. WeissS, "Istherea neurakcellinthemammalian forebrain", TrendsNeurosci, 19, pp.387~393 (1996)
  37. GageFH, "Mammalianneuralstem cell", Science, 287, pp.1433~1438 (2000)
  38. paganoSF, "Isolationandcharacterization of neural stem cells from the adult human olfactory bulb", stem cells, 18, pp.295~300 (2000)
  39. RaoMS, "Multipotentandrestrictedprecursors in the centralnervous system", Anat. Rec, 257, pp.137~148 (1999)
  40. ReynoldsBA, "A multipotent EGF-responsive striatal embryonic progenitor cell produces neuronsandastrocyte", J.Neurosci.Res, 12, pp.4565~4574 (1992)
  41. Kornblum HI, "Introduction to Neural Stem Cells", Stroke, 38(2), pp.810~816 (2007)
  42. MichalczykK, "Nestinstructure and predicted function in cellular cytoskeletalorganisation", HistolHospital, 20(52), pp.665~671 (2005)
  43. 권광원, "신경줄기세포의 냉동보관법 확립", Anatomy and Cell Biology, 37(6), pp.499~508 (2004), 대한해부학회
  44. 조명수, "배아줄기세포의 신경세포로의 분화", 인구의학연구논집, 16, pp.7~13 (2003)
  45. Hockfield S, "Identification ofmajorcellclassesin thedeveloping mammaliannervoussystem", JNeurosci, 5, pp.3310~3328 (1985)
  46. LendahlU, "CNSstem cellsexpressanew classof intermediatefilamentprotein", Cell, 60, pp.585~595 (1990)
  47. OkabeS, "Developmentofneural precursorcellsandfunctionalpostmitotic neuronsfrom embryonicstem cellsin vitro", Mechanism Develop, 59, pp.89~102 (1996)
  48. ZhangSC, "Invitrodifferentiation of transplantable neural precursors from human embryonicstem cells", Nat Biotechnol, 19, pp.1129~1133 (2001)
  49. Kilpatrick TJ, "Cloning and growthofmultipotentialneuralprecursor. Requirements for proliferation and differentiation", Neuron, 10, pp.255~265 (1993)
  50. MurphyMJ, "Fibroblast growthfactorstimulatestheproliferation and differentiation ofneuralprecursor cellsin vitro", J.Neurosci.Res, 25, pp.463~475 (1990)
  51. GossJR, "Astrocytes arethemajorsource ofnervegrowth factorupregulation following traumatic brain injury in the rat", Exp Neurol, 22, pp.3231~3326 (2000)
  52. CaltinMC, "Effects ofethanolon clacium homeostasis in the nervous system: implications for astrocytes", MolNeurobiol, 19, pp.1~14 (1999)
  53. BenvenisteEN, "Tumornecrosisfactor-α enhancesinterferon-γ mediated classⅡ antigen expression onastrocytes", JNeuroimmunol, 25, pp.209~219 (1989)
  54. 오현주, "문헌에 나타난 향기요법에 대한 고찰", 한방재활학회지, 7(1), pp.546~560 (1997)
  55. 張介賓, "張氏景岳全書", pp.610~611 (1978), 현성사
  56. 孫思邈, "千金要方", pp.315~ (1995), 대성문화사
  57. 王壽, "外臺秘要", pp.249~254 (1975), 성보사
  58. 趙佶, "聖濟總錄43卷", pp.822~825 (1987), 人民衛生出版社
  59. 龔廷賢, "壽世保元", pp.290~293 (1995), 上海科學技術出版社
  60. 李時珍, "本草綱目", pp.883~887 (1982), 人民衛生出版社
  61. 李仲梓, "醫宗必讀", pp.76~ (1978), 大方出版社
  62. 김용진, "당귀가 백서의 뇌손상 및 심혈관계에 미치는 영향", 대한한의학회지, 21(4), pp.37~46 (2000)
  63. 정종길, "當歸", pp.12~15 (2005), 醫林323
  64. 李沆雨, "土川芎과 日川芎의 效能및 品質比較에 관한 硏究-두種類의 川芎과 川芎·當歸配合時의 血管弛緩效能", 大韓本草學會誌, 14(1), pp.55~60 (1999)
  65. LeeS, "Anti-tumoractivities of decursinolangelateand decursin from Angelica gigas", Arch.Pharm.Res, 26, pp.727~730 (2003)
  66. 柳康秀, "참당귀根의 Coumarin成分에 관한 연구", 약학회지, 11, pp.22~ (1967)
  67. Marshall,NJ, "A criticalassessmentoftheuseofmicroulture tetazolium assaystomeasurecellgrowth andfunction", Growthregul, 5, pp.69~84 (1995)
  68. 의 발현, "대한병리학회지", 2001, 361~367
  69. 정병갑, "분자세포생물학 제2판", pp.506~ (2002), 도서출판 한우리
  70. FrankeTF, "定志小丸對抑鬱模型大鼠齒狀回神經干細胞和學習記憶能力的影響", Cell, 88, pp.435~437 (1997)
  71. ShiueH, "Akt2 phosphorylates ezrin to trigger NHE3 translocation andactivation", JBiolChem, 280(2), pp.1688~1695 (2005)
  72. HiraoM, "Regulation mechanism of ERM protein", plasma membrane association :Possible involvement of phosphatidylturnoverandRho-dependent signalingpathway, 1996, pp.135, 37~51
  73. Cobb MH, "How MAP kinaseareregulated", JBiolChem, 270, pp.14843~14846 (1995)
  74. Chuderland D, "Protein-protein interactions in the regulation of the extracellularsignal-regulatedkinase", MolBiotechnol, 29, pp.57~74 (2005)
  75. Shamloo M, "Activation ofthe extracellularsignalregulated protein kinase cascade in thehippocampalCA1region in arat model of global cerebral ischemic preconditioning", Neuroscience, 93, pp.81~88 (1999)
  76. Magnuson NS, "The Raf-1serine", threoninekinase, 1994, pp.5, 247~253

관련 논문AI추천

이 간행물의 다른 논문 (15건)