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鷄血藤이 Rat의 말초신경 재생에 미치는 효과

Effect of Gyehyuldeung Treatments in Peripheral Nerve Regeneration of Rat

유호룡|김윤식|안정조|조현경|설인찬|임승민
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2009.06|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제30권 제2호|pp. 375-387

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참고문헌

키워드

GAP-43Gyehyuldeungaxonal regenerationCdc2 protein
주제분류한의학

초록

Objective : Gyehyuldeung (GHD) has been widely used in oriental medicine for the treatments of cardiovascular and neurological disorders. Thus, its potential facilitatory activity on axonal regeneration was investigated in the rats. Methods : Sprague-Dawley rats were given crush injury at the sciatic nerve and the changes of axon growth after nerve injury on each nerve injury model were investigated with anti-NF-200 antibody, DiI, GAP-43 protein and Cdc2 protein Results : GHD-mediated enhancement of axonal regeneration after crush injury was measured in both qualitative and quantitative ways by immunofluorescence staining with anti-NF-200 antibody and retrograde tracing of fluorescence dye DiI. GAP-43 protein levels were elevated by GHD treatments in the distal injured sciatic nerve and DRG sensory neurons. The neurite outgrowth of DRG sensory neurons was facilitated by GHD treatment when co-cultured with Schwann cells and astrocytes prepared from injured sciatic nerves and injured spinal cord tissues, respectively. It was observed that Cdc2 protein was up-regulated in co-cultured Schwann cells or astrocytes and Cdc2 protein signals were co-localized to a certain extent with those of phospho-vimentin protein. Conclusions : These results suggest that GHD may play a facilitatory role in axonal regeneration by acting on the injured axons and adjacent non-neuronal cells. The current findings may be useful for the development of therapeutic targets through more specific explorations on molecular interactions between herbal components and endogenous factors.

Abstract

Objective : Gyehyuldeung (GHD) has been widely used in oriental medicine for the treatments of cardiovascular and neurological disorders. Thus, its potential facilitatory activity on axonal regeneration was investigated in the rats. Methods : Sprague-Dawley rats were given crush injury at the sciatic nerve and the changes of axon growth after nerve injury on each nerve injury model were investigated with anti-NF-200 antibody, DiI, GAP-43 protein and Cdc2 protein Results : GHD-mediated enhancement of axonal regeneration after crush injury was measured in both qualitative and quantitative ways by immunofluorescence staining with anti-NF-200 antibody and retrograde tracing of fluorescence dye DiI. GAP-43 protein levels were elevated by GHD treatments in the distal injured sciatic nerve and DRG sensory neurons. The neurite outgrowth of DRG sensory neurons was facilitated by GHD treatment when co-cultured with Schwann cells and astrocytes prepared from injured sciatic nerves and injured spinal cord tissues, respectively. It was observed that Cdc2 protein was up-regulated in co-cultured Schwann cells or astrocytes and Cdc2 protein signals were co-localized to a certain extent with those of phospho-vimentin protein. Conclusions : These results suggest that GHD may play a facilitatory role in axonal regeneration by acting on the injured axons and adjacent non-neuronal cells. The current findings may be useful for the development of therapeutic targets through more specific explorations on molecular interactions between herbal components and endogenous factors.

참고문헌 (30)

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관련 논문AI추천

Growth Promoting Effects of Oriental Medicinal Drugs on Sciatic Nerve Regeneration in the Rat남궁욱, 설인찬, 조현경, Yoon Sik Kim한의병리학회 · 동의생리병리학회지2005 · 제19권 제6호Sengmaek-san-mediated Enhancement of Axonal Regeneration after Sciatic Nerve Injury in the Rat김윤식, 남궁욱, 백경민, 설인찬, 안정조, 유호룡, 조현경한의병리학회 · 동의생리병리학회지2008 · 제22권 제2호補氣除痛湯減味方의 랫드 말초신경 손상에 대한 회복 효과 연구김진미, 김철중, 조충식대한한방내과학회 · 대한한방내과학회지2012 · 제33권 제1호보기제통탕이 말초신경병증 모델에서 신경 손상 회복에 미치는 영향김철중, 박상우, 조충식대한한방내과학회 · 대한한방내과학회지2013 · 제34권 제4호Effects of Oriental Medicinal Drugs on Axonal Regeneration in the Spinal Cord Neurons남궁욱, 설인찬, 안정조, Yoon-Sik Kim한의병리학회 · 동의생리병리학회지2005 · 제19권 제6호중추신경계 손상 회복에 미치는 대한 조구등의 영향김동웅, 박형진, 송봉근, 이진구대한약침학회 · Journal of Pharmacopuncture2009 · 제12권 제1호

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