사물탕, 사군자탕, 팔물탕, 십전대보탕의 약리 활성 비교 연구
Immuno stimulatory activities of Samul-tang, Sagunja-tang, Pamul-tang and Sipjeondaebo-tang in vitro
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Objectives :Samul-tang(SM), Sagunja-tang(SG), Pamul-tang(PM) and Sipjeondaebo-tang(SJ) was used many diseases such as sterility, menstrual disorder, general prostration, recruitment in Korea. We investigated the immune stimulatory activities of SG, SM, PM and SJ in in vitro. Methods :For comparision for effective of SM, SG, PM and SJ, this study examined anti-inflammation(NO, PGE2 assay), anti-oxidation(DPPH assay) and immune response in in vitro assay. For immune response activities, this study used NO synthesis on RAW 264.7 cells, splenocyte proliferation and cytokine assay(IL-2, IL-4) in splenocyte. Results :The results showed that SG, SM, PM and SJ were no significant effect anti-oxidation and anti-inflammatory effects. For immune response, they showed the splenocyte proliferation and macrophage proliferation. We confirmed that they synthesised NO a dose-dependent manner significantly and secreted the IL-4. Conclusions :These results suggested the SG, SM, PM and SJ had immune stimulatory activity. A efficacy of immune response in them had produced similar results.
Abstract
Objectives :Samul-tang(SM), Sagunja-tang(SG), Pamul-tang(PM) and Sipjeondaebo-tang(SJ) was used many diseases such as sterility, menstrual disorder, general prostration, recruitment in Korea. We investigated the immune stimulatory activities of SG, SM, PM and SJ in in vitro. Methods :For comparision for effective of SM, SG, PM and SJ, this study examined anti-inflammation(NO, PGE2 assay), anti-oxidation(DPPH assay) and immune response in in vitro assay. For immune response activities, this study used NO synthesis on RAW 264.7 cells, splenocyte proliferation and cytokine assay(IL-2, IL-4) in splenocyte. Results :The results showed that SG, SM, PM and SJ were no significant effect anti-oxidation and anti-inflammatory effects. For immune response, they showed the splenocyte proliferation and macrophage proliferation. We confirmed that they synthesised NO a dose-dependent manner significantly and secreted the IL-4. Conclusions :These results suggested the SG, SM, PM and SJ had immune stimulatory activity. A efficacy of immune response in them had produced similar results.
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