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인간 신경모세포종 SH-SY5Y에서 人蔘 total ginsenosides의 신경보호 기능에 관련된 유전자 발현 양상에 대한 연구

Gene expression profiling of SH-SY5Y cells in neuroprotective effect of total ginsenosides on H2O2 induced neurotoxicity

이승기|정경화|김지혁|허용석|채영규
대한한방신경정신과학회 (The Korean Society Of Oriental Neuropsychiatry)|2007.03|동의신경정신과학회지 (The Korean Society of Oriental Neuropsychiatry)|제18권 제1호|pp. 95-110

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Abstract

Objective : The purpose of this study was to investigate molecular basis of neuroprotective effect in total ginsenosides. After H2O2 induced neurotoxicity, gene expression profiling of SH-SY5Y neuroblastoma cells treated by total ginsenosides is analyzed.Method : After SH-SY5Y cells were cultured, they were damaged by H2O2 induced oxidative stress. After twenty four hours, experimental group is treated by total ginsenosides and control group is treated by 0.9% saline. A high density cDNA microarray chip is used to analyze the gene expression profiling of SH-SY5Y cells. The Significance Analysis of Microarray method is used for identifying genes on a microarray. Results: 1. According to the results of microarray experiment, 17 genes were up-regulated, 38 genes were down-regulated. 2. Expression of OPHN1, KTAN1, ATM, PRKCE, MAPKs genes associated with cell proliferation, neural growth, and the prevention of apoptosis were increased. 3. Change of EPX gene was the greatest among all genes. EPX gene associated with oxidative stress, and tumor suppressor gene ADAM11 were decreased. Conclusion : According to this study, molecular basis of neuroprotective effect of total ginsenosides is as followings: the increase of gene expression associated with cell proliferation, neuron growth, the prevention of apoptotsis and decrease of gene expression associated with oxidative stress and tumor suppressor.

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