蜈蚣의 항산화효과에 관한 연구
Antioxidative Effects of Scolopendra subspinipes
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초록
Objective : The purpose of this study was to evaluate the antioxidative effects of the extract of Scolopendra subspinipes which has been used mainly for detoxication in the oriental medicine and reported to have sedative action, antiinflammatory effect, antihypertensive property and immunity enhancing activity. Method : Inhibitory activities on oxygen radical generating enzymes (aldehyde oxidase and xanthine oxidase) and increasing activities on oxygen radical scavenging enzymes (superoxide dismutase, glutathione peroxidase, glutathione-S-transferase) were investigated. Furthermore, the content of glutathione in the mouse brain, DPPH radical scavenging activity and also anti-lipid peroxidative effects in vivo and in vitro were estimated. Results : The extract showed weak inhibitory effects on the activities of aldehyde oxidase and xanthine oxidase which are oxygen radical generating enzymes. The extract inhibited lipid peroxidation with 26.1% against control group at 500 mg/kg in vivo and with 11.2% against control group at 10 mg/kg in vitro in a dose-dependent manner, which means this drug may protect radical-induced cell damages. The extract showed dose-dependently the scavenging effect on DPPH radical with 24.8% activity at 10 mg/ml in vitro. The extract enhanced the activities of superoxide dismutase, glutathione peroxidase and glutathione-S-transferase, which are oxygen radical scavenging enzymes, with 28.9%, 22.3% and 23.1%, respectively at 500mg/kg in vivo. Finally, this extract strongly increased the glutathione content in the mouse barin. Conclusion : Above results indicated that Scolopendra subspinipes can be useful for the protection or treatment of some diseases caused by reactive oxygen species.
Abstract
Objective : The purpose of this study was to evaluate the antioxidative effects of the extract of Scolopendra subspinipes which has been used mainly for detoxication in the oriental medicine and reported to have sedative action, antiinflammatory effect, antihypertensive property and immunity enhancing activity. Method : Inhibitory activities on oxygen radical generating enzymes (aldehyde oxidase and xanthine oxidase) and increasing activities on oxygen radical scavenging enzymes (superoxide dismutase, glutathione peroxidase, glutathione-S-transferase) were investigated. Furthermore, the content of glutathione in the mouse brain, DPPH radical scavenging activity and also anti-lipid peroxidative effects in vivo and in vitro were estimated. Results : The extract showed weak inhibitory effects on the activities of aldehyde oxidase and xanthine oxidase which are oxygen radical generating enzymes. The extract inhibited lipid peroxidation with 26.1% against control group at 500 mg/kg in vivo and with 11.2% against control group at 10 mg/kg in vitro in a dose-dependent manner, which means this drug may protect radical-induced cell damages. The extract showed dose-dependently the scavenging effect on DPPH radical with 24.8% activity at 10 mg/ml in vitro. The extract enhanced the activities of superoxide dismutase, glutathione peroxidase and glutathione-S-transferase, which are oxygen radical scavenging enzymes, with 28.9%, 22.3% and 23.1%, respectively at 500mg/kg in vivo. Finally, this extract strongly increased the glutathione content in the mouse barin. Conclusion : Above results indicated that Scolopendra subspinipes can be useful for the protection or treatment of some diseases caused by reactive oxygen species.
참고문헌 (35)
- Fridovich I, "The biology of oxygen radicals", Science, 201, pp.875~ (1978)
- Marttila RJ, "Oxygen toxicity protecting enzymes in Parkinson's disease", J. Neurol. Sci, 86, pp.321~ (1988)
- Johannsen P, "Glutathione peroxidase in early and advanced Parkinson's disease", J. Neurol. Neurolsurg. Psychiat, 54, pp.679~ (1991)
- Zelman FA, "Superoxide Dismutase activity in Alzheimer's disease. possible mechanism for paired helical filament formation", Brain. Res, 476, pp.160~ (1988)
- Frlich I, "Free Radical mechanisms in Dementia of Alzheimer Type and the Potential for Antioxidative Treatment", Arzneim-Forsch. Drug Res, 45, pp.443~ (1995)
- Pellegrini-Giampietro DE, "Excitatory amino acid release and free radicals formation may cooperate in the genesis of ischemia-induced neuronal damage", J. Neurosci, 10, pp.1035~ (1990)
- Halliwell B, "Reactive oxygen species and the central nervous system", J. Neurochem, 59, pp.1609~ (1992)
- Hall ED, "The role of oxygen radical-induced lipid peroxidation in acute CNS trauma. Oxygen radicals and tissue injury", pp.92~ (1988), FASEB Bethesda, M.D.
- Marx JL, "Oxygen free radicals linked to many diseases", Science, 35, pp.529~531 (1987)
- Harman DJ, , Gerontol, 11, pp.298~ (1956)
- Gilman SC, "Effect of oxidative stress on excitatory amino acid release by cerebral cortical synaptesomes", Free Rad. Biol. & Med, 15, pp.671~ (1993)
- 馬繼興, "神農本草經輯主", pp.449~ (1995), 人民衛生出版社
- 신민교, "원색임상본초학", pp.665~666 (1991), 영림사
- 김종희, "오공(蜈蚣)의 진통, 소염, 진경 및 독성작용에 관 한 실험적 연구", 대한한의학회지, 14 (1993)
- 이동희, "蜈蚣의 항고혈합 작용에 관한 연구", 대한본초학회지, 12(2), pp.39~49 (1997)
- 김길섭, "蜈蚣이 老齡에 따른 mouse의 면역 기능에 미치는 영향", 대한한방내과학회지, 19(1), pp.477~490 (1998)
- 안규석, "蚯蚓, 水蛭, 蠐螬 및 蜈蚣이 血栓症에 미치는 영향", 대한한의학회지, 11(2), pp.92~101 (1990)
- Rajagopalan KV, "Hepatic aldehyde oxidase. I. Purification and properties", J. Biol. Chem, 237, pp.922~ (1962)
- Stirpe F, "The regulation of rat liver xanthine oxidase : Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O)", J. Biol. Chem, 244, pp.3855~ (1969)
- Ohkawa H, "Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction", Anal. Biochem, 95, pp.351~ (1979)
- Blosi MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~ (1958)
- Martin JP, "Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation", Arch. Biochem. Biophys, 255, pp.329~ (1987)
- Paglia DE, "Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase", Lab. Clin. Med, 70, pp.158~ (1967)
- Lawrence RA, "Glutathione peroxidase activity in selenium-dificient rat liver", Biochem. Biophysi. Res. Comm, 71, pp.952~ (1976)
- Habig WH, "Glutathione-S-transferase: the first enzymatic step mercapturic acid formation", J. Biochem, 249 (1974)
- Ellman GL, "Tissue sulfhydryl group", Arch. Biochem. Biophys, 82, pp.70~77 (1959)
- 전국한의과대학본초학교실, "본초학", pp.508~ (1991), 영림사
- 이상인, "본초학", pp.239~240 (1981), 수서원
- 이상인, "한약임상응용" (1986), 성보사
- 張樹生, "百藥效用奇觀", pp.87~ (1987), 中國古籍出版社
- 笵崔生, "中藥的應用", pp.370~ (1989), 人民衛生出版社
- 楊永良, "中藥學", pp.232~ (1989), 湖北科學技術出版社
- 江蘇新醫學院篇, "中藥大辭典", pp.2472~ (1979), 上海科學技術出版社
- 黎新源, "熱鬱胸膈, 鼻堉辨治", pp.1~ (1990), 新中醫
- 이시진, "도해본초강목", pp.1324~1325 (1975), 고문사