In vitro에서 β-site amyloid precursor protein-cleaving enzyme 활성과 amyloid β protein 생산에 대한 聰明湯加味方의 효과
Effect of Chongmyung -Tang Prescription Combination on the Production of Amyloid β protein and β-site amyloid precursor protein-cleaving enzyme Activity in vitro
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초록
Objectives :This experiment was designed to investigate the effect of Chongmyung-Tang Prescription Combination(CmTP-C1-10) extract on the production of amyloid β protein and β-site amyloid precursor protein-cleaving enzyme(BACE) activity. Methods :The effect of CmTP-C1-10 extract on expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by lipopolysacchride(LPS) and amyloid β protein fragment(Aβ fragment) were investigated. The effect of CmTP-C1-10 extract on production of amyloid β protein(Aβ) in BV2 microglia cell line treated by LPS and Aβ fragment were investigated. The effect of CmTP-C1-10 extract on BACE activity were investigated. Results :1. CmTP-C9 extract the most significantly suppressed the expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by LPS and Aβ fragment. 2. CmTP-C9 extract significantly suppressed the production of Aβ in BV2 microglia cell line treated by LPS and Aβ fragment. 3. CmTP-C9 extract the most significantly inhibited BACE activity. Conclusions :These results suggest that CmTP-C9 may be effective for the prevention and treatment of Alzheimer's Disease. Investigation into clinical use of CmTP-C9 for Alzheimer's Disease is suggested for future research.
Abstract
Objectives :This experiment was designed to investigate the effect of Chongmyung-Tang Prescription Combination(CmTP-C1-10) extract on the production of amyloid β protein and β-site amyloid precursor protein-cleaving enzyme(BACE) activity. Methods :The effect of CmTP-C1-10 extract on expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by lipopolysacchride(LPS) and amyloid β protein fragment(Aβ fragment) were investigated. The effect of CmTP-C1-10 extract on production of amyloid β protein(Aβ) in BV2 microglia cell line treated by LPS and Aβ fragment were investigated. The effect of CmTP-C1-10 extract on BACE activity were investigated. Results :1. CmTP-C9 extract the most significantly suppressed the expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by LPS and Aβ fragment. 2. CmTP-C9 extract significantly suppressed the production of Aβ in BV2 microglia cell line treated by LPS and Aβ fragment. 3. CmTP-C9 extract the most significantly inhibited BACE activity. Conclusions :These results suggest that CmTP-C9 may be effective for the prevention and treatment of Alzheimer's Disease. Investigation into clinical use of CmTP-C9 for Alzheimer's Disease is suggested for future research.
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