천죽황(Bambusae concretio silicae, 天竺黃) 물추출물이 신경전달물질 수용체의 표현에 미치는 영향
Effects of Water-Extracts of Bambusae concretio silicae(BCS, 天竺黃) on the Expression of Neurotransmitter Receptors
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Objectives :BCS(Bambusae concretio silicae) is used as a traditional medicine in Korea for the incipient stroke. Recent reports indicated that BCS has a neuroprotective effect by anti-convulsion effect. However, it's mechanism is not well studied. The purpose of this study was to investigate into the molecular mechanism of BCS for neuroprotection in normoxia of cultured rat hippocampal neurons. Methods :BCS (1.0, 2.5, 5.0, and 10.0 ㎍/㎖) was added to culture media (Neurobasal supplemented with B27) on DIV 0, given a normoxia, and the cell viability was measured by typical phase-contrast images of the cultures with 1.0, 2.5, 5.0, and 10.0 ㎍/㎖ on DIV 21. Effects of BCS on the expression of various synaptic proteins (GABAB R1, GABAB R2, GlyR, PSD95) were observed by immunocytochemistry showing on DIV 3, 7 and 21. Results :Typical phase-contrast images of the cultures indicated that BCS has a protective effect of rat hippocampal cells in normoxia. The BCS upregulated GABAB R1 after normoxia on DIV 7, GABAA β2/3 on DIV 21 and GABAB R2 on DIV 21. And the BCS downregulated PSD95 after normoxia on DIV 7. Conclusions :The present study showed evidence for the efficacy of BCS in Typical phase-contrast images, upregulation of inhibitory neurotransmitter receptors(GABAB R1) and downregulation of PSD95 which eventually protected neuronal cell death in normoxia.
Abstract
Objectives :BCS(Bambusae concretio silicae) is used as a traditional medicine in Korea for the incipient stroke. Recent reports indicated that BCS has a neuroprotective effect by anti-convulsion effect. However, it's mechanism is not well studied. The purpose of this study was to investigate into the molecular mechanism of BCS for neuroprotection in normoxia of cultured rat hippocampal neurons. Methods :BCS (1.0, 2.5, 5.0, and 10.0 ㎍/㎖) was added to culture media (Neurobasal supplemented with B27) on DIV 0, given a normoxia, and the cell viability was measured by typical phase-contrast images of the cultures with 1.0, 2.5, 5.0, and 10.0 ㎍/㎖ on DIV 21. Effects of BCS on the expression of various synaptic proteins (GABAB R1, GABAB R2, GlyR, PSD95) were observed by immunocytochemistry showing on DIV 3, 7 and 21. Results :Typical phase-contrast images of the cultures indicated that BCS has a protective effect of rat hippocampal cells in normoxia. The BCS upregulated GABAB R1 after normoxia on DIV 7, GABAA β2/3 on DIV 21 and GABAB R2 on DIV 21. And the BCS downregulated PSD95 after normoxia on DIV 7. Conclusions :The present study showed evidence for the efficacy of BCS in Typical phase-contrast images, upregulation of inhibitory neurotransmitter receptors(GABAB R1) and downregulation of PSD95 which eventually protected neuronal cell death in normoxia.
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