본문 바로가기
KERD
논문KCI 등재

성심산(醒心散)의 중대뇌동맥 폐쇄로 유발된 허혈성 뇌손상 백서(白鼠)에 대한 인지 및 운동기능 회복 촉진효과

Sungshim-san-mediated Recovery of Cognition and Motor Function in the Severe Rat Stroke, Permanent Middle Cerebral Artery Occlusion Model

정대규|이경석
대한한방신경정신과학회 (The Korean Society Of Oriental Neuropsychiatry)|2015.09|동의신경정신과학회지 (The Korean Society of Oriental Neuropsychiatry)|제26권 제3호|pp. 319-336

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

Sungshim-sanpMCAOLimb placing testWater maze testDonepezil.
주제분류한의학

초록

Objectives: The object of this study was to evaluate the cognition and motor function recovery effects of Sungshim-san (SSS), a traditional Korean cardio-protective polyherbal formula in the severe rat stroke, permanent middle cerebral artery occlusion (pMCAO) model.Methods: The experimental animals were divided into 6 groups. SSS aqueous extracts (yield=16.82%; 400, 200 and 100 mg/kg) were administered orally by using Sonde, once daily, for 28 continuous days from 24 hrs post-pMCAO. Donepezil10 mg/kg, a representative drug for dementia, was used as a refer-ence drug. The body weight changes, infarct/defect sizes, sensorimotor function and cognitive motor behavior were serially monitored. Limb placing and body-swing test for sensorimotor functions were conducted at 1 day before operation (base line), and 1, 3, 7, 14, 21 and 28 days post-pMCAO; and water maze test for the cognitive motor behavior was conducted at 14 and 28 days post-pMCAO, respectively.Results: Focal cerebral cortex infarct and defects due to pMCAO resulted in marked decreases of body weight, disorders of sensorimotor functions and cognitive motor behaviors. However, the pMCAO-re-lated ischemic damages were markedly and dose-dependently inhibited by treatment with SSS 400 and 200 mg/kg, respectively. Donepezil markedly decreased the body weight and gains, as compared with pMCAO control rats; however, SSS 400 and 200 mg/kg favorably ameliorated the pMCAO-in-duced decreases in body weight and gains. SSS 100 mg/kg treated rats did not show any favorable ef-fects on the pMCAO-related ischemic damages, as compared with pMCAO control rats.Conclusions: The results of the study indicated that oral administration of SSS 400 and 200 mg/kg ac-celerated cognition and motor function recovery in the rat pMCAO model. The treatment effect was potentially mediated by neuroprotection via the known augmentation of cerebral antioxidant defense system of SSS itself or its individual herbal components. Especially, the overall effects of SSS 200 mg/kg were similar to those of donepezil 10 mg/kg, but less toxic.

Abstract

Objectives: The object of this study was to evaluate the cognition and motor function recovery effects of Sungshim-san (SSS), a traditional Korean cardio-protective polyherbal formula in the severe rat stroke, permanent middle cerebral artery occlusion (pMCAO) model.Methods: The experimental animals were divided into 6 groups. SSS aqueous extracts (yield=16.82%; 400, 200 and 100 mg/kg) were administered orally by using Sonde, once daily, for 28 continuous days from 24 hrs post-pMCAO. Donepezil10 mg/kg, a representative drug for dementia, was used as a refer-ence drug. The body weight changes, infarct/defect sizes, sensorimotor function and cognitive motor behavior were serially monitored. Limb placing and body-swing test for sensorimotor functions were conducted at 1 day before operation (base line), and 1, 3, 7, 14, 21 and 28 days post-pMCAO; and water maze test for the cognitive motor behavior was conducted at 14 and 28 days post-pMCAO, respectively.Results: Focal cerebral cortex infarct and defects due to pMCAO resulted in marked decreases of body weight, disorders of sensorimotor functions and cognitive motor behaviors. However, the pMCAO-re-lated ischemic damages were markedly and dose-dependently inhibited by treatment with SSS 400 and 200 mg/kg, respectively. Donepezil markedly decreased the body weight and gains, as compared with pMCAO control rats; however, SSS 400 and 200 mg/kg favorably ameliorated the pMCAO-in-duced decreases in body weight and gains. SSS 100 mg/kg treated rats did not show any favorable ef-fects on the pMCAO-related ischemic damages, as compared with pMCAO control rats.Conclusions: The results of the study indicated that oral administration of SSS 400 and 200 mg/kg ac-celerated cognition and motor function recovery in the rat pMCAO model. The treatment effect was potentially mediated by neuroprotection via the known augmentation of cerebral antioxidant defense system of SSS itself or its individual herbal components. Especially, the overall effects of SSS 200 mg/kg were similar to those of donepezil 10 mg/kg, but less toxic.

참고문헌 (70)

  1. Feigin VL, "Stroke epidemiology : a review of population-based studies of incidence, prevalence, and case-fatality in the late 20th century", Lancet Neurol, 2, pp.43~53 (2003)
  2. Kim YS., "Intergrated clinical approach to stroke", pp.303~308 (1997), Seowondang
  3. 장원석, "六君子湯이 Glutamate에 의한 C6 신경교세포의 Apoptosis에 미치는 영향", 대한한방내과학회지, 31(3), pp.586~599 (2010), 대한한방내과학회
  4. Kirino T, "Selective vulnerability of the hippocampus to ischemia-reversible and irreversible types of ischemic cell damage", Progress in Brain Res, 63, pp.39~58 (1985)
  5. 정광식, "淸肺瀉肝湯이 MCAO에 의한 흰쥐의 뇌허혈 손상에 미치는 영향", 대한예방한의학회지, 13(1), pp.13~27 (2009), 대한예방한의학회
  6. 김영태, "茵蔯淸肝湯의 알코올성 산화스트레스에 대한 보호효과 연구", 대한한방내과학회지, 32(4), pp.550~564 (2011), 대한한방내과학회
  7. Watanabe H, "Pharmacological evidence for antidementia effect of Choto-san(Goutengsan), a traditional Kampo medicine", Pharmacol Biochem Behav, 75, pp.635~643 (2003)
  8. McCarty MF, "Potential complementarity of high-flavanol cocoa powder and spirulina for health protection", Med Hypotheses, 74, pp.370~373 (2010)
  9. Harada S, "Preventive effect of Morinda citrifolia fruit juice on neuronal damage induced by focal ischemia", Biol Pharm Bull, 32, pp.405~409 (2009)
  10. Birks J, "Donepezil for dementia due to Alzheimer's disease", Cochrane Database Syst Rev, (1), pp.CD001190~ (2006)
  11. Rogers SL, "Perspectives in the management of Alzheimer's disease : clinical profile of donepezil", Dement Geriatr Cogn Disord, 9, pp.29~42 (1998)
  12. Román GC, "Donepezil : a clinical review of current and emerging indications", Expert Opin Pharmacother, 5, pp.161~180 (2004)
  13. 최성훈, "혈관성치매에 대한 한·양방 치료약물의 처방 사례연구", 대한예방한의학회지, 15(3), pp.39~49 (2011), 대한예방한의학회
  14. Dunn NR, "Adverse effects associated with the use of donepezil in general practice in England", J Psychopharmacol, 14, pp.406~408 (2000)
  15. Takasaki K, "Neuroprotective effects of citidine–5-diphosphocholine on impaired spatial memory in a rat model of cerebrovascular dementia", J Pharmacol Sci, 116, pp.232~237 (2011)
  16. Hu D, "Schizandrin, an antioxidant lignan from Schisandra chinensis, ameliorates Aβ1-42-induced memory impairment in mice", Oxid Med Cell Longev, pp.721~ (2012)
  17. Kim WC, "A clinical study of vascular dementia in stroke patients", Journal of korean oriental internal medicine, 19, pp.50~59 (1998)
  18. 최성훈, "피질하 혈관성치매의 진단, 치료 및 예방:1례 보고와 함께", 대한예방한의학회지, 14(3), pp.63~75 (2010), 대한예방한의학회
  19. Heart-systemic internal med. Dep. of College of Ori. med, "Oriental heart-systemic internal medicine", pp.425~ (1999), Seowondang
  20. Sim GO., "Sim Ssijonsaengseo", pp.137~ (1979), Jayu publisher
  21. Heo J., "Dongui bogam", pp.245~ (2005), Dongeuibogam publisher
  22. Kwak JM, "A Study on the Effects of Sungshimsan on the Heart Lipid Peroxide and Metabolic Enzyme System in Senescence Induced Rats", Daejeon Univ. journal of oriental medicine, 8, pp.625~641 (1999)
  23. Park YK, "An Experimental Study on The Effect of Seongshimsan", Kyunghui medicine, 5(4), pp.424~431 (1989)
  24. Lee BK, "Oriental diagnostics", pp.II66~ (2000), Sungbosa
  25. De Ryck M, "Photochemical stroke model : flunarizine prevents sensorimotor deficits after neocortical infarcts in rats", Stroke, 20, pp.1383~1390 (1989)
  26. Borlongan CV, "Elevated body swing test : a new behavioral parameter for rats with 6-hydroxydop amine-induced hemiparkinsonism", J Neurosci, 15, pp.5372~5378 (1995)
  27. Morris RGM, "Development of a water maze procedure for studying spatial memory in the rat", J Neurosci Methods, 11, pp.47~60 (1984)
  28. Tamura A, "Focal cerebral ischaemia in the rat: 1.description of technique and early neuropathological consequences following middle cerebral artery occlusion", J Cereb Blood Flow Metab, 1, pp.53~60 (1981)
  29. Lee JY, "Ibudilast, a phosphodiesterase inhibitor with anti-inflammatory activity, protects against ischemic brain injury in rats", Brain Res, 1431, pp.97~106 (2012)
  30. Levene A., "Pathological factors influencing excision of tumours in the head and neck", Part I. Clin Otalary, 6, pp.145~151 (1981)
  31. Ludbrook J, "Update: microcomputer statistics packages. A personal view", Clin Exp Pharmacol Physiol, 24, pp.294~296 (1997)
  32. Cucchiara B, "Transient ischemic attack: risk stratification and treatment", Ann Emereg Med, 52, pp.S27~S39 (2008)
  33. Rossini PM, "Post-stroke plastic reorganisation in the adult brain", Lancet Neurol, 2, pp.493~502 (2003)
  34. Lukic-Panin V, "Prevention of neuronal damage by calcium channel blockers with antioxidative effects after transient focal ischemia in rats", Brain Res, 1176, pp.143~150 (2007)
  35. Bisson JF, "Effects of long-term administration of a cocoa polyphenolic extract(Acticoa powder)on cognitive performances in aged rats", Br J Nutr, 100, pp.94~101 (2008)
  36. Head E, "Oxidative damage and cognitive dysfunction : antioxidant treatments to promote healthy brain aging", Neurochem Res, 34, pp.670~678 (2009)
  37. Wattanathorn J, "Zingiber officinale mitigates brain damage and improves memory impairment in focal cerebral ischemic rat", Evid Based Complement Alternat Med, pp.429~505 (2011)
  38. Calapai G, "Neuroprotective effects of Ginkgo biloba extract in brain ischemia are mediated by inhibition of nitric oxide synthesis", Life Sci, 67, pp.2673~2683 (2000)
  39. Suk K, "Neuroprotection by methanol extract of Uncaria rhynchophylla against global cerebral ischemia in rats", Life Sci, 70, pp.2467~2480 (2002)
  40. Koo BS, "Protective effects of alpinae oxyphyllae fructus(Alpinia oxyphylla MIQ)water-extracts on neurons from ischemic damage and neuronal cell toxicity", Phytother Res, 18, pp.142~148 (2004)
  41. Zhang JB, "Zhange Jingyue", pp.640~ (1999), Jeongdam publisher
  42. "Herbal medical prof. co-editor. Herbal medicine", pp.588~622 (1994), Younglimsa
  43. Kwan-Ho Jang, "Neuroprotective Effect of Ginseng radix on ICH - induced Rats", 대한한의학회지, 26(4), pp.87~97 (2005), 대한한의학회
  44. 조익현, "Effects of Panax ginseng in Neurodegenerative Diseases", Journal of Ginseng Research, 36(4), pp.342~353 (2012), 고려인삼학회
  45. 이태희, "구속 스트레스로 유발된 기억 손상에 대한 麥門冬의 신경보호 효능", 대한본초학회지, 21(2), pp.63~75 (2006), 대한본초학회
  46. 문정민, "五味子 추출물의 인간 전립선암 세포주 PC-3에 대한 성장 억제 효과", 대한본초학회지, 27(4), pp.17~23 (2012), 대한본초학회
  47. In-hwan Son, "Protective Effects of Phenolic-rich Fraction(PRF) from Fructus Schisandrae on H2O2-induced Apoptosis of SH-SY5Y Cells", 대한한방내과학회지, 28(2), pp.230~241 (2007), 대한한방내과학회
  48. juyeonban, "Methanol Extract of Polygalae Radix Protects Excitotoxicity in Cultured Neuronal Cells", 한국약용작물학회지, 11(4), pp.298~305 (2003), 한국약용작물학회
  49. Young-Giun Kim, "Neuroprotective Effect of Aqueous Extract of Polygala tenuifolia Willdenow on Nitric Oxide-induced Apoptosis in SK-N-MC Cells", 대한한의학회지, 31(3), pp.55~65 (2010), 대한한의학회
  50. 신길조, "흰쥐 대뇌세포의 저산소증 모델에서 석창포(石菖蒲 Acori graminei rhizoma. AGR)에 의한 유전자 표현 변화의 microarray 분석", 생명과학회지, 17(2), pp.150~161 (2007), 한국생명과학회
  51. 이성률, "遠志와 石菖蒲 混合抽出液의 pCT105로 유도된 신경세포암 세포주에 대한 항치매 효과", 동의생리병리학회지, 17(4), pp.1037~1049 (2003), 한의병리학회
  52. Hwang YK, "Effect of the neuroprotetion and anti-Alzheimer's disease in CT99-induced Neuro 2A cells by Ikgiansintang water extract", Herbal formula science, 13, pp.103~121 (2005)
  53. Bederson JB, "Rat middle cerebral artery occlusion : evaluation of the model and development of a neurologic examination", Stroke, 17, pp.472~476 (1986)
  54. Yamamoto M, "Behavioral changes after focal cerebral ischemia by left middle cerebral artery occlusion in rats", Brain Res, 452, pp.323~328 (1988)
  55. Roof RL, "A comparison of long-term functional outcome after 2 middle cerebral artery occlusion models in rats", Stroke, 32, pp.2648~2657 (2001)
  56. Menniti FS, "Phosphodiesterase 5A inhibitors improve functional recovery after stroke in rats : optimized dosing regimen with implications for mechanism", J Pharmacol Exp Ther, 331, pp.842~850 (2009)
  57. Hunter AJ, "To what extent have functional studies of ischaemia in animals been useful in the assessment of potential neuroprotective agents", Trends Pharmacol Sci, 19, pp.59~66 (1998)
  58. Nishino H, "Pathophysiological process after transient ischemia of the middle cerebral artery in the rat", Brain Res Bull, 35, pp.51~56 (1994)
  59. Witte OW, "Functional differentiation of multiple perilesional zones after focal cerebral ischemia", J Cereb Blood Flow Metab, 20, pp.1149~1165 (2000)
  60. Umegaki H, "Discontinuation of donepezil for the treatment of Alzheimer's disease in geriatric practice", Int Psychogeriatr, 20, pp.800~806 (2008)
  61. Schöller K, "Neuroprotection in ischemic stroke-combination drug therapy and mild hypothermia in a rat model of permanent focal cerebral ischemia", Brain Res, 1023, pp.272~278 (2004)
  62. Wang Z, "Safety of low-frequency transcranial ultrasound in permanent middle cerebral artery occlusion in spontaneously hypertensive rats", Cerebrovasc Dis, 33, pp.23~29 (2012)
  63. Kufemann IL, "Principles of neural science", pp.806~815 (1986), Elservier
  64. Seo JH, "Simultaneous Stain with Neutral Red and Tetrazolium Salt for Experimental Cerebral Infarction Morphometry", Journal of Korean Neurosurgical Society, 21(8), pp.990~998 (1992)
  65. Jaspers RM, "Spatial learning is affected by transient occlusion of common carotid arteries(2VO) : comparison of behavioural and histopathological changes after ‘2VO’ and ‘four-vesselocclusion’in rats", Neurosci Lett, 117, pp.149~153 (1990)
  66. Yamaguchi T, "YM796, a novel muscarinic agonist, improves the impairment of learning behavior in a rat model of chronic focal cerebral ischemia", Brain Res, 669, pp.107~114 (1995)
  67. Colbourne F, "Delayed and prolonged post-ischemic hypothermia is neuroprotective in the gerbil", Brain Res, 654, pp.265~272 (1994)
  68. Kawamata T, "Intracisternal basic fibroblast growth factor(bFGF)enhances behavioral recovery following focal cerebral infarction in the rat", J Cereb Blood Flow Metab, 16, pp.542~547 (1996)
  69. Ritz MF, "Chronic treatment with red wine polyphenol compounds mediates neuroprotection in a rat model of ischemic cerebral stroke", J Nutr, 138, pp.519~525 (2008)
  70. Bednar MM, "The role of sildenafil in the treatment of stroke", Curr Opin Investig Drugs, 9, pp.754~759 (2008)

관련 논문AI추천

朱雀丸이 일시적 국소 뇌허혈 유발 白鼠에 대한 인지 및 운동기능 회복에 미치는 효과강석봉, 김보은, 정대규대한한방신경정신과학회 · 동의신경정신과학회지2013 · 제24권 제4호Neuroprotective Effects of Guh-Poong-Chung-Sim-Hwan on Focal Cerebral Ischemia in Rats김정범, 임하섭한의병리학회 · 동의생리병리학회지2005 · 제19권 제1호허혈성 뇌손상 백서에서 加減聰明湯이 인지기능에 미치는 효과김경윤, 김계엽, 김행중, 김형우, 이상영, 이석진, 정현우, 차대연한의병리학회 · 동의생리병리학회지2008 · 제22권 제3호성향정기산이 흰쥐의 MCAO에 의한 국소뇌허혈에 미치는 영향김연섭, 김효선한의병리학회 · 동의생리병리학회지2006 · 제20권 제3호뇌졸중후 인지기능저하의　한약치료에　대한　임상적　고찰권정남, 김영균, 김재규, 민성순, 손연희, 신철경, 정현윤, 허정은대한약침학회 · Journal of Pharmacopuncture2008 · 제11권 제4호淸肺瀉肝湯이 MCAO에 의한 흰쥐의 뇌허혈 손상에 미치는 영향김범회, 정광식, 황원덕대한예방한의학회 · 대한예방한의학회지2009 · 제13권 제1호

이 간행물의 다른 논문 (9건)

五行 특성을 바탕으로 한 五神의 기능에 대한 고찰박선영pp. 201-209한의사 직종에 대한 한의대생의 인식 연구 - 성별에 따른 차이를 중심으로정하룡, 이재혁pp. 211-224한국인 어지럼증 환자의 최근 4년간 한방 진료 양태김재영, 박삼민, 황동규, 고영탁, 정선영pp. 225-234한의학 칠정(七情)에 기반을 둔 핵심감정평가척도를 이용한 공황장애 환자의 특성 연구유소정, 박종민, 강형원, 류영수pp. 235-249Trends of Tuina Therapy on Depression and Its Efficacy -based on CNKI-Eun-Ji Choi, Chan-Young Kwon, 김종우, 정선용pp. 251-265
원문 보기