액체크로마토그래피-텐덤질량분석법에 의한 하천수 및 정수 중 남조류 기원 신종 독소 BMAA(β-N-Methylamino-L-Alanine)의 정량분석
Determination of β-N-Methylamino-L-Alanine (BMAA) Levelsin Surface Water and Treated Water by Liquid Chromatography-Tandem Mass Spectrometry
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
β-Methylamino-L-alanine (BMAA) is an emerging algal toxin that has drawn attention because it can cause neurodegenerative diseases, including Parkinson's disease and Alzheimer's disease (AD). Therefore, it is necessary to develop a sensitive analytical method for BMAA, an emerging cyanobacterial toxin, to determine whether a trace amount is detected in the water resources and treated water. In this study, we developed a simple and sensitive analytical method for BMAA using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with direct injection. The method was validated for linearity of calibration, limit of detection, limit of quantitation, accuracy, and precision. The limits of detection and quantitation were in the range of 0.030 ?/L and 0.096 ?/L, respectively. The optimized method was used to measure the concentrations of BMAA in surface water from each weir point of the Geum River, Nakdong River. Sampling and analysis of drinking water treatment plants of the Geum River, Nakdong River were applied to samples for each process.
Abstract
β-Methylamino-L-alanine (BMAA) is an emerging algal toxin that has drawn attention because it can cause neurodegenerative diseases, including Parkinson's disease and Alzheimer's disease (AD). Therefore, it is necessary to develop a sensitive analytical method for BMAA, an emerging cyanobacterial toxin, to determine whether a trace amount is detected in the water resources and treated water. In this study, we developed a simple and sensitive analytical method for BMAA using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with direct injection. The method was validated for linearity of calibration, limit of detection, limit of quantitation, accuracy, and precision. The limits of detection and quantitation were in the range of 0.030 ?/L and 0.096 ?/L, respectively. The optimized method was used to measure the concentrations of BMAA in surface water from each weir point of the Geum River, Nakdong River. Sampling and analysis of drinking water treatment plants of the Geum River, Nakdong River were applied to samples for each process.
참고문헌 (17)
- J. Hu, "Exposure to Aerosolized Algal Toxins in South Florida Increases Short-and Long-Term Health Risk in Drosophila Model of Aging", Toxins, 12, pp.787~ (2020)
- J. Stanhope, "Epidemiologic features of amyotrophic lateral sclerosis and parkinsonism-dementia in Guam, mariana islands", Int. J. Epidemiol, 1, pp.199~210 (1972)
- J. F. Amanda, "The analysis of underivatized β-Methylamino-L-alanine (BMAA), BMAA, AEG & 2,4-DAB in Pteropus mariannus mariannus specimens using HILIC-LC-MS", MS, 2018, pp.152, 150~159
- Cyanotoxins: methods and approaches for their analysis and detection, "European Commission", European Commission, 8~15 (2017)
- N. Chernoff, "A critical review of the postulated role of the non-essential amino acid, β-N-methylamino-L-alanine, in neurodegenerative disease in humans", J. Toxicol. Environ. Heal. Part B Crit. Rev., 20, pp.183~229 (2017)
- E. J. Faassen, "A collaborative evaluation of LC-MS", MS based methods for BMAA analysis: soluble bound BMAA found to be an important fraction, 2016, pp.14, 45~
- E. J. Faassen, "Presence of the Neurotoxin BMAA in Aquatic Ecosystems : What Do We Really Know?", Toxins(Basel), 6, pp.1109~1138 (2014)
- E. J. Faassen, "A comparative study on three analytical methods for the determination of the neurotoxin BMAA in cyanobacteria", PLoS One, 5, pp.e36667~ (2012)
- B. Yan, "Optimization of the determination method for dissolved cyanobacterial toxin BMAA in natural water", Anal. Chem., 2017, pp.89~
- A. J. Fossa, "The analysis of underivatized β-Methylamino-L-alanine (BMAA), BAMA, AEG and 2,4-DAB in Pteropus mariannus mariannus specimens using HILIC-LC-MS", MS, 2018, pp.15, 150~159
- A. Combes, "Development of an analytical procedure for quantifying the underivatized neurotoxin β-N-methylamino-L-alanine in brain tissues", Anal. Bioanal. Chem., pp.4627~4636 (2014)
- D. G. Beach, "Differential mobility-mass spectrometry double spike isotope dilution study of release of β-methylaminoalanine and proteinogenic amino acids during biological sample hydrolysis", Sci. Rep., 8, pp.117~ (2018)
- 환경부, "조류경보제 운영 매뉴얼", ~ (2016)
- 환경부, "먹는물 수질감시항목 운영 등에 관한 고시", 108~137 (2018)