소나무잎중 PCDD/Fs 정량시 SPMDs 와 GPC를 이용한 지질 제거법 비교

Abstract

This study is on a comparison between GPC and SPMDs methods, the popular ones for removing lipid from pine needles which have been used for bio-monitoring of atmospheric persistent organic pollutants (POPs), such as PCDD/Fs, PCBs, PAHs, PBDEs and so on, and is fatty enough to make the analysis difficult. The results are the following: 1. The average 13C PCDD/Fs recovery of SPMDs was 98.6% (81.1-127.5%), 19.1% higher than that of GPC, 79.5% (72.2-86.2%). 2. The residual lipid in samples after SPMDs was about one half as much as that after GPC. 3. During the supplementary procedure using multi-layer silica to remove the residual lipid, the samples after SPMDs consumed 12% more solvent and 47% less silica than those after GPC did. The amount of relatively expensive and toxic DCM consumed after SPMDs, however, was 31% less than that after GPC, which shows that the SPMDs method was frugal and good for safety in work. 4. It took 48 hours to dialyze during SPMDs, while it took 4-5 hours to elute during GPC. Although the SPMDs method takes longer for a single or several samples, there is a good advantage in using the SPMDs method for the large number of samples since it may dialyze several scores of samples at the same time.