HPLC 분석에서 컬럼유속과 이동상 용매강도의 변화에 따른 카보닐 표준시료의 검출특성
Detection Characteristics of a HPLC Method in Carbonyl-DNPH Derivatives in Relation with Column Flow and with Solvent Stenegth of Mobile Phase
1
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
A number of carbonyl compounds in the atmosphere such as ketones and aldehydes can harmfully affect the human health as primary and secondary pollutants, respectively. In order to analyze these compounds, one of the most reliable methods include the analysis of their 2, 4-dinitrophenylhydrazine (DNPH) derivatives through an application a reversed-phase High Performance Liquid Chromatographic (RP-HPLC) technique. The main purposes of this study are to examine the detection characteristics of the HPLC method against carbonyl-DNPH derivatives in relation with column flow and solvent strength of mobile phase. The overall results of our study indicate that changes in column flow sensitively affect the detectability, while solvent strength influences more on their resolution.
참고문헌 (13)
- Müller, K, "제목없음", Chemosphere, 35(9), pp.2093~2106 (1997)
- 여현구, "제목없음", 대기환경공학회, 24(10), pp.1711~1721 (2002)
- 정지연, "제목없음", 한국산업위생학회지, 10(1), pp.126~146 (2000)
- Kuwata, K, "제목없음", Journal of Chromatography A,, 211(3), pp.378~382 (1979)
- G.Andersson, "제목없음", Chemosphere, 8(10), pp.823~827 (1979)
- R. K.Beasley, "제목없음", Anal. Chem., 52(7), pp.1110~1114 (1980)
- "Method for the determination of aldehyde and ketone in ambient air using high performance liquid chromatography (HPLC), method TO-5.", Environmental Protection Agency (EPA), ~ (1984)
- "Method for the determination of formaldehyde in ambient air using adsorbent cartridge followed by high performance liquid chromatography (HPLC), method TO-11.", Environmental Protection Agency (EPA), ~ (1987)
- "Method for the determination of formaldehyde in ambient air using adsorbent cartridge followed by high performance liquid chromatography (HPLC) method TO-11A (Active Sampling Methodology).", Environmental Protection Agency (EPA), ~ (1999)
- 최영욱, "제목없음", 대한화학회지, 43(4), pp.438~446 (1999)
- 문경환, "실내 공기 중 일부 알데하이드류에 대한 위해도 평가-일부 주택 및 아토피 환자 주택을 대상으로", 한국환경보건학회지, 32(1), pp.19~26 (2006), 한국환경보건학회
- Grandy K, "제목없음", Atmo. Environ., 31(10), pp.1403~1415 (1997)
- 김기현, "대기 중 카보닐 계열 성분의 분석기법의 연구: 포름알데하이드와 DNPH의 반응 특성을 중심으로", 분석과학, 18(1), pp.43~50 (2005), 한국분석과학회