Bacterial Diversity in Soil Surround Subterranean Termites-Damaged Wooden Buildings in Seonamsa Temple and Effect of the Termites on Bacterial Diversity in Humus Soil
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In order to determine the changes in microbial community due to termites, soil microorganisms surrounding the termites were investigated. First, bacterial communities from soil with termites collected at Seonamsa temple, Suncheon city, Korea were compared by next-generation sequencing (NGS, Illumina Miseq). The bacterial composition of soil from Daeungjeon without termites and the soil from Josadang, Palsangjeon, and Samjeon with termites were compared. Next, the bacterial composition of these soils was also compared with that of humus soil cultured with termites. A total high-quality sequences of 71,942 and 72,429 reads were identified in Seonamsa temple’s soil and humus soil, respectively. The dominant phyla in the collected Seonamsa temple’s soil were Proteobacteria (27%), Firmicutes (24%) and Actinobacteria (21%), whereas those in the humus soil were Bacteriodetes (56%) and Proteobacteria (37%). Using a two-dimensional plot to explain the principal coordinate analysis of operational taxonomic unit compositions of the soil samples, it was confirmed that the samples were divided into soil with and without termites, and it was especially confirmed that the Proteobacteria phylum was increased in humus soil with termites than in humus soil without termites.
Abstract
In order to determine the changes in microbial community due to termites, soil microorganisms surrounding the termites were investigated. First, bacterial communities from soil with termites collected at Seonamsa temple, Suncheon city, Korea were compared by next-generation sequencing (NGS, Illumina Miseq). The bacterial composition of soil from Daeungjeon without termites and the soil from Josadang, Palsangjeon, and Samjeon with termites were compared. Next, the bacterial composition of these soils was also compared with that of humus soil cultured with termites. A total high-quality sequences of 71,942 and 72,429 reads were identified in Seonamsa temple’s soil and humus soil, respectively. The dominant phyla in the collected Seonamsa temple’s soil were Proteobacteria (27%), Firmicutes (24%) and Actinobacteria (21%), whereas those in the humus soil were Bacteriodetes (56%) and Proteobacteria (37%). Using a two-dimensional plot to explain the principal coordinate analysis of operational taxonomic unit compositions of the soil samples, it was confirmed that the samples were divided into soil with and without termites, and it was especially confirmed that the Proteobacteria phylum was increased in humus soil with termites than in humus soil without termites.
참고문헌 (13)
- Caporaso, J. G., "Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms", Journal of the International Society for Microbial Ecology, 6, pp.1621~1624 (2012)
- Egert, M., "Microbial community structure in midgut and hindgut of the humus-feeding larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae)", Applied and Environmental Microbiology, 69(11), pp.6659~6668 (2003)
- Fall, S., "Differences between bacterial communities in the gut of a soil-feeding termite (Cubitermes niokoloensis) and its mounds", Applied and Environmental Microbiology, 73(16), pp.5199~5208 (2007)
- Grieco, M. B., "Metagenomic analysis of the whole gut microbiota in Brazilian Termitidae termites Cornitermes cumulans, Cyrilliotermes strictinasus, Syntermes dirus, Nasutitermes jaraguae, Nasutitermes aquilinus, Grigiotermes bequaerti, and Orthognathotermes mirim", Current Microbiology, 76(6), pp.687~697 (2019)
- Husseneder, C., "Comparison of the bacterial symbiont composition of the Formosan subterranean termite from its native and introduced range", The Open Microbiology Journal, 4, pp.53~66 (2010)
- 김영희, "탐지견 반응 및 환경 인자 분석을 통한 목조 건축물의 흰개미 피해 조사", 보존과학회지, 35(6), pp.641~651 (2019), 한국문화재보존과학회[DOI]
- "Land information platform" (2021)[URL]
- 임보아, "선암사 조사당의 흰개미 피해 및 환경 특성 통계 분석", 보존과학회지, 35(3), pp.197~208 (2019), 한국문화재보존과학회[DOI]
- Manjula, A., "Comparative analysis of microbial diversity in termite gut and termite nest using ion sequencing", Current Microbiology, 72, pp.267~275 (2016)
- Manjula, A., "Microbial diversity in termite nest", Current Science, 106(10), pp.1430~1434 (2014)
- Ohkuma, M., "Phylogenetic diversity of the intestinal bacterial community in the termite Reticulitermes speratus", Applied and Environmental Microbiology, 62(2), pp.461~468 (1996)
- Rossmassler, K., "Metagenomic analysis of the microbiota in the highly compartmented hindguts of six wood- or soil-feeding higher termites", Microbiome, 3(56), pp.1~6 (2015)
- Thongaram, T., "Comparison of bacterial communities in the alkaline gut segment among various species of higher termites", Extremophiles, 9(3), pp.229~238 (2005)