국내 인체피부모델을 이용한 광독성 동물대체시험법 개발 연구
Development of an In vitro Phototoxicity Test Using the Human Reconstituted Epidermis Model Developed in Korea
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Abstract
Human reconstituted epidermis models have been widely used in evaluating phototoxicity. However, human reconstituted epidermis models made in Korea have not been employed to assess phototoxicity. In this study, we conducted an in vitro phototoxicity test with Neoderm® -E, human reconstituted epidermis model from Korea, in order to see if the model could be used for the evaluation of phototoxicity. Firstly, we performed an UVA sensitivity test and selected 6 J/cm^2 for an UVA irradiation dose. Chlorpromazine as a positive control was then tested to confirm that the substance induces phototoxicity in this model. Afterwards, 3 phototoxins and 2 non-phototoxins were tested using Neoderm® -E and Epiderm TM , a generally used phototoxicity test model, to compare prediction results for each chemical. The results indicated that all those 5 chemicals were correctly predicted with Neoderm® -E, which was accordant with the phototoxic information on them. However, only 3 chemicals were correctly predicted with Epiderm TM . Consequently, the predictive capacity of the test with Neoderm® -E could be higher than that using Epiderm TM . Overall, we reached the conclusion that Neoderm® -E could be used in evaluating phototoxicity.
참고문헌 (29)
- 김민지, "인공피부모델 KeraSkinTM을 이용한 삼황사심탕의 피부자극 평가", 한국동물실험대체법학회지, 7, pp.5~9 (2013)
- 문지영, "인공피부모델 KeraSkinTM 피부자극성 시험 대체시험법의 실험실간 검증", 한국동물실험대체법학회지, 4, pp.5~13 (2010)
- 박양희, "인공피부모델을 이용한 트라넥삼산 제형의 피부투과도 및 자극평가", 한국동물실험대체법학회지, 6, pp.27~29 (2012)
- 박정은, "한국형 인공피부조직을 이용한 탐색적 피부과민성 대체시험법 개발", 한국동물실험대체법학회지, 9, pp.39~43 (2015)
- 이내경, "인공 피부, 인공 각막 모델을 활용한 동물실험 대체법 연구의 최근 동향", 한국동물실험대체법학회지, 8, pp.29~36 (2014)
- 장원희, "인공피부모델을 이용한 보습파우더의 보습 효능 평가", 한국동물실험대체법학회지, 5, pp.57~60 (2011)
- 조지훈, "국내 개발 인공피부의 친염증성 사이토카인 발현 평가", 한국동물실험대체법학회지, 7, pp.35~38 (2013)
- 조지훈, "국내 개발 인공피부 배양액 및 조직용해액내 염증매개단백질 발현 수준 평가에 대한 초기 연구", 한국동물실험대체법학회지, 9, pp.33~37 (2015)
- Bernard, F. X., "Development of a highly sensitive in vitro phototoxicity assay using the SkinEthicTM reconstructed human epidermis", Cell biology and toxicology, 16, pp.391~400 (2001)
- Bin, B. H., "Novel inhibitory effect of N-(2-hydroxycyclohexyl)valiolamine on melanin production in a human skin model", International journal of molecular sciences, 15, pp.12188~12195 (2014)
- 조경아, "Interleukin-17 and Interleukin-22 Induced Proinflammatory Cytokine Production in Keratinocytes via Inhibitor of Nuclear FactorκB Kinase-α Expression", Annals of Dermatology, 24(4), pp.398~405 (2012), 대한피부과학회
- 최종혜, "Skin Corrosion and Irritation Test of Sunscreen Nanoparticles using Reconstructed 3D Human Skin Model", 환경독성보건학회지, 29, pp.1~10 (2014), 환경독성보건학회
- EMA, "Note for guidance on photosafety testing", EMA, ~ (2002)
- EURL ECVAM, "EURL ECVAM status report on the development, validation and regulatory acceptance of alternative methods and approaches (2015)", European Union, ~ (2015)
- Gould, J. W., "Cutaneous photosensitivity diseases induced by exogenous agents", Journal of the american academy of dermatology, 33, pp.551~573 (1995)
- ICH, "Photosafety evaluation of pharmaceuticals S10 step 4. ICH", ~ (2013)
- Jones, P. A., "In vitro testing for phototoxic potential using the epiderm 3-D reconstructed human skin model", Toxicology methods, 11, pp.1~19 (2001)
- Jung, K. M., "KeraskinTM-VM : a novel reconstructed human epidermis model for skin irritation tests", Toxicology in vitro, 28, pp.742~750 (2014)
- Lelièvre, D., "The episkin phototoxicity assay(EPA) : Development of an in vitro tiered strategy using 17 reference chemicals to predict phototoxicity potency", Toxicology in vitro 21, pp.977~995 (2007)
- Liebsch, M., "Development of a new in vitro test for dermal phototoxicity using a model of reconstituted human epidermis", Altex, 14, pp.165~174 (1997)
- Netzlaff, F., "The human epidermis models Episkin®, SkinEthic® and EpiDerm® : an evaluation of morphology and their suitability for testing phototoxicity, irritancy, corrosivity, and substance transport", European journal of pharmaceutics and biopharmaceutics, 60, pp.167~178 (2005)
- OECD, "OECD guideline for testing of chemicals, Section 4: Health effects. Test No. 432. In vitro 3T3 NRU phototoxicity test", OECD, ~ (2004)
- OECD, "OECD guideline for testing of chemicals, Section 4: Health effects. Test No. 431. In vitro skin corrosion: reconstructed human epidermis (RHE) test method", OECD, ~ (2015)
- OECD, "OECD guideline for testing of chemicals, Section 4: Health effects. Test No. 439. In vitro skin irritation: reconstructed human epidermis test method", OECD, ~ (2015)
- Onoue S., "High-throughput reactive oxygen species(ROS)assay : An enabling technology for screening the phototoxic potential of pharmaceutical substance", Journal of pharmaceutical and biomedical analysis, 46, pp.187~193 (2008)
- Portes, P., "Use of human reconstituted epidermis EpiSkin® for assessment of weak phototoxic potential of chemical compounds", Photodermalogy, photoimmunology and photomedicine, 18, pp.96~102 (2002)
- Poumay, Y., "Modelling the human epidermis in vitro : tools for basic and applied research", Archives of dermatological research, 298, pp.361~369 (2007)
- Spielmann, H., "The second ECVAM workshop on phototoxicity testing", The report and recommendations of ECVAM workshop, 42, pp.777~814 (2000)
- ZEBET, "Phototoxicity protocol for use with EpidermTM model (EPI-200) final version (5th November 1997)", ZEBET, 1997