HaCaT 세포와 돼지 피부를 이용한 보습효능평가 비교연구
Comparison Study of Moisturizing Efficacy in HaCaT Cell and MicropigⓇ Skin
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This study was performed to compare the moisturizing efficacy between HaCaT cell and MicropigⓇ skin. Dry skin condition was induced by SDS in HaCaT cell and MicropigⓇ skin, and retinoic acid (RA), adenosine (AD) and ceramide (CER) were used for standard substances of moisturizing evaluation. In HaCaT cells, after setting the substance concentration through MTT assay, dry skin condition was induced by treatment of sodium dedecyl sulfate (SDS). In order to confirm the improvement of dryness by the standard substances in SDS-induced HaCAT cells, the expression of hyaluronic acid (HA), aquaporin-3 (AQP3) and hyaluronic acid synthase-2 (HAS2) proteins was measured. As for MicropigⓇ skin, transepidermal water loss (TEWL) was used to establish the protective effect for dry skin induction by SDS in MicropigⓇ. The difference value before and after treatment of standard materials was compared. In conclusion, it suggested that the test methods and conditions could be set efficiently by using three standard substances suitable for moisturizing effect characteristics at the cellular and tissue level. Also it can be said that the criteria for evaluating the moisturizing effect of moisturizing ingredients have been well established.
Abstract
This study was performed to compare the moisturizing efficacy between HaCaT cell and MicropigⓇ skin. Dry skin condition was induced by SDS in HaCaT cell and MicropigⓇ skin, and retinoic acid (RA), adenosine (AD) and ceramide (CER) were used for standard substances of moisturizing evaluation. In HaCaT cells, after setting the substance concentration through MTT assay, dry skin condition was induced by treatment of sodium dedecyl sulfate (SDS). In order to confirm the improvement of dryness by the standard substances in SDS-induced HaCAT cells, the expression of hyaluronic acid (HA), aquaporin-3 (AQP3) and hyaluronic acid synthase-2 (HAS2) proteins was measured. As for MicropigⓇ skin, transepidermal water loss (TEWL) was used to establish the protective effect for dry skin induction by SDS in MicropigⓇ. The difference value before and after treatment of standard materials was compared. In conclusion, it suggested that the test methods and conditions could be set efficiently by using three standard substances suitable for moisturizing effect characteristics at the cellular and tissue level. Also it can be said that the criteria for evaluating the moisturizing effect of moisturizing ingredients have been well established.
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