접골목 추출물에 의한 항산화 활성이 정상 간세포의t-BHP 유발 산화스트레스에 미치는 영향
Antioxidant Activity and Protective Effects of Extracts from Sambucus williamsii var. coreana on t-BHP Induced Oxidative Stress in Chang cells
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In the present study, antioxidant activity and protective effect of extracts from Sambucus williamsii var. coreanastems (SWC) were evaluated on tert-butyl hydroperoxide (t-BHP) induced oxidative stress in human liver (Chang)cells. Antioxidant activities of the SWC extracts were determined by various radical scavenging activities, suchas DPPH, ferric reducing antioxidant power (FRAP), 2,2’-azinobis-(3-ethybenzothiazoline-6-sulfonic acid)(ABTS) radical scavenging activity and oxygen radical absorbance capacity (ORAC) assay. SWC extracts showedstrong antioxidant effect on various assay. To determine the hepatoprotective effects of SWC on t-BHP inducedoxidative damage, cell viability was measured using MTT assay. Pretreatment of SWC extracts showed increasingcell viability, decreasing ROS and restoring mitochondria membrane potential on t-BHP induced oxidative stressin Chang cells. Our findings suggest that SWC extracts may be considered a potential agent for therapeuticprotective effect from oxidative stress through its antioxidant activity.
Abstract
In the present study, antioxidant activity and protective effect of extracts from Sambucus williamsii var. coreanastems (SWC) were evaluated on tert-butyl hydroperoxide (t-BHP) induced oxidative stress in human liver (Chang)cells. Antioxidant activities of the SWC extracts were determined by various radical scavenging activities, suchas DPPH, ferric reducing antioxidant power (FRAP), 2,2’-azinobis-(3-ethybenzothiazoline-6-sulfonic acid)(ABTS) radical scavenging activity and oxygen radical absorbance capacity (ORAC) assay. SWC extracts showedstrong antioxidant effect on various assay. To determine the hepatoprotective effects of SWC on t-BHP inducedoxidative damage, cell viability was measured using MTT assay. Pretreatment of SWC extracts showed increasingcell viability, decreasing ROS and restoring mitochondria membrane potential on t-BHP induced oxidative stressin Chang cells. Our findings suggest that SWC extracts may be considered a potential agent for therapeuticprotective effect from oxidative stress through its antioxidant activity.
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