Impact of Microbial Infection on Sperm Parameters of Seminal Bacteria in Asymptomatic Subfertile Males
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Purpose: This study examined the effects of asymptomatic bacteriospermia on the semen quality of subfertile males. The types of bacteria and their antibiotic susceptibility were also analyzed. Materials and Methods: Semen was collected and analyzed from 510 subfertile males. One hundred and seventy-nine males showed bacteriospermia, while 331 males did not. The bacterial species, sperm parameters, hormone levels, underlying disease, and lifestyle patterns were compared between the two study groups. Results: The bacteriospermic males showed significantly higher rates of leukocytospermia (p=0.001) and deoxyribonucleic acid (DNA) fragmentation than the non-bacteriospermic males. Sperm motility was significantly lower in the bacteriospermic males than in non-bacteriospermic males. The most common seminal bacterial species were Prevotella bivia (P. bivia, 41.3%) and Ureaplasma urealyticum (U. urealyticum, 13.4%). U. parvum showed the highest recurrence rates (31.8%) three months after the initial antibiotic treatment. Regarding the sperm parameters of bacteriospermic males, the sperm concentration, total motility, progressive motility, leukocytospermia, and DNA fragmentation were improved significantly after the initial antibiotics treatment. Multivariate logistic regression analyses revealed P. bivia, U. urealyticum, and U. parvum to be associated with the decreased motility and increased DNA fragmentation of spermatozoa. P. bivia was also associated with a decreased sperm concentration (p=0.002) and vitality (p=0.013). Conclusions: Bacteriospermia decreased the sperm concentration, motility, normal morphology, and vitality. P. bivia is the most commonly observed bacteria in subfertile males. Appropriate antibiotic therapy of seminal bacteria species had a strong positive impact on improving the semen parameters.
Abstract
Purpose: This study examined the effects of asymptomatic bacteriospermia on the semen quality of subfertile males. The types of bacteria and their antibiotic susceptibility were also analyzed. Materials and Methods: Semen was collected and analyzed from 510 subfertile males. One hundred and seventy-nine males showed bacteriospermia, while 331 males did not. The bacterial species, sperm parameters, hormone levels, underlying disease, and lifestyle patterns were compared between the two study groups. Results: The bacteriospermic males showed significantly higher rates of leukocytospermia (p=0.001) and deoxyribonucleic acid (DNA) fragmentation than the non-bacteriospermic males. Sperm motility was significantly lower in the bacteriospermic males than in non-bacteriospermic males. The most common seminal bacterial species were Prevotella bivia (P. bivia, 41.3%) and Ureaplasma urealyticum (U. urealyticum, 13.4%). U. parvum showed the highest recurrence rates (31.8%) three months after the initial antibiotic treatment. Regarding the sperm parameters of bacteriospermic males, the sperm concentration, total motility, progressive motility, leukocytospermia, and DNA fragmentation were improved significantly after the initial antibiotics treatment. Multivariate logistic regression analyses revealed P. bivia, U. urealyticum, and U. parvum to be associated with the decreased motility and increased DNA fragmentation of spermatozoa. P. bivia was also associated with a decreased sperm concentration (p=0.002) and vitality (p=0.013). Conclusions: Bacteriospermia decreased the sperm concentration, motility, normal morphology, and vitality. P. bivia is the most commonly observed bacteria in subfertile males. Appropriate antibiotic therapy of seminal bacteria species had a strong positive impact on improving the semen parameters.
참고문헌 (22)
- Gnoth C, "Definition and prevalence of subfertility and infertility", Hum Reprod, 20, pp.1144~1147 (2005)
- Agarwal A, "A unique view on male infertility around the globe", Reprod Biol Endocrinol, 13, pp.37~ (2015)
- Han H, "Aetiology of obstructive azoospermia in Chinese infertility patients", Andrologia, 48, pp.761~764 (2016)
- Lackner J, "Value of counting white blood cells(WBC)in semen samples to predict the presence of bacteria", Eur Urol, 49, pp.148~152 (2006)[DOI]
- Punab M, "The limit of leucocytospermia from the microbiological viewpoint", Andrologia, 35, pp.271~278 (2003)
- Pajovic B, "Semen analysis before and after antibiotic treatment of asymptomatic Chlamydia-and Ureaplasma-related pyospermia", Andrologia, 45, pp.266~271 (2013)
- Zhang F, "Role of Lactobacillus in female infertility via modulating sperm agglutination and immobilization", Front Cell Infect Microbiol, 10, pp.620529~ (2021)
- Fraczek M, "Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa : potential inflammatory markers in semen", Folia Histochem Cytobiol, 53, pp.201~217 (2015)
- Allow AK, "Sperm agglutination, sperm shaky head movement and sperm-cervical interaction tests could be enough for diagnosis of immunological infertility?", J Gynecol Womens Health, 3, pp.555603~ (2017)
- Volz Y, "Asymptomatic bacteriospermia and infertility-what is the connection?", Infection, 50, pp.1499~1505 (2022)
- Gimenes F, "Male infertility : a public health issue caused by sexually transmitted pathogens", Nat Rev Urol, 11, pp.672~687 (2014)
- Fraczek M, "The effect of bacteriospermia and leukocytospermia on conventional and nonconventional semen parameters in healthy young normozoospermic males", J Reprod Immunol, 118, pp.18~27 (2016)
- Wogatzky J, "The combination matters--distinct impact of lifestyle factors on sperm quality : a study on semen analysis of 1683 patients according to MSOME criteria", Reprod Biol Endocrinol, 10, pp.115~ (2012)
- Pluddemann A, "Can drinking more water prevent urinary tract infections? The evidence says yes", BMJ Evid Based Med, 24, pp.191~192 (2019)
- Eini F, "Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia", BMC Mol Cell Biol, 22, pp.42~ (2021)
- Domes T, "The incidence and effect of bacteriospermia and elevated seminal leukocytes on semen parameters", Fertil Steril, 97, pp.1050~1055 (2012)
- Machado A, "Influence of biofilm formation by Gardnerella vaginalis and other anaerobes on bacterial vaginosis", J Infect Dis, 212, pp.1856~1861 (2015)
- Farahani L, "The semen microbiome and its impact on sperm function and male fertility : a systematic review and meta-analysis", Andrology, 9, pp.115~144 (2021)
- Bai S, "Association of sexually transmitted infection with semen quality in men from couples with primary and secondary infertility", Asian J Androl, 24, pp.317~322 (2022)
- Toren P, "Effect of dutasteride on clinical progression of benign prostatic hyperplasia in asymptomatic men with enlarged prostate : a post hoc analysis of the REDUCE study", BMJ, 346, pp.f2109~ (2013)
- Said MA, "The impact of 5α-reductase inhibitor use for male pattern hair loss on men's health", Curr Urol Rep, 19, pp.65~ (2018)
- La Vignera S, "Does alcohol have any effect on male reproductive function? A review of literature", Asian J Androl, 15, pp.221~225 (2013)