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LPS로 유도한 복강대식세포에서 IκB-α 분해억제에 의한 柴梗半夏湯의 항염증효과

Shigyungbanha-tang Exhibits Anti-inflammatory Effects by Inhibiting IκB-α Degradation in LPS-stimulated Peritoneal Macrophages

이시형|신조영|이승언
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2007.09|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제28권 제3호|pp. 442-452

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Abstract

Objectives : The purpose of this study was to investigate the toll-like receptor (TLR)-4 mediated anti-inflammatory effects of extract from Shigyungbanha-tang (SBT) on the peritoneal macrophage.Methods : To evaluate of TLR-4 mediated inflammatory of SBT, we examined NO and cytokine production in TRL-4 ligand (LPS : lipopolysaccharide) induced macrophages. Furthermore, we examined its molecular mechanism using western blot.Results : Extract from SBT itself does not have any cytotoxic effect in the peritoneal macrophages. Extract from SBT reduced LPS-induced nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6 and IL-12 production in peritoneal macrophages. SBT inhibited degradation of inhibitor kappa B-alpha (IκB-α) in the TLR-4 mediated peritoneal macrophages.Conclusions : These results suggest that SBT inhibits NO and cytokines production through inhibiting nuclear factor-kappaB (NF-κB) activation in peritoneal macrophage and that SBT may be beneficial oriental medicine for inflammation

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