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조골세포 세포사멸의 Estrogen 조절에 대한 Hsp27의 영향에 관한 연구

Hsp27 contributes to estrogen regulation of osteoblast apoptosis

최철민|윤정주|임재석|권종진|장현석
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2004.08|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제30권 제4호|pp. 323-330

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Abstract

Estrogen may promote osteoblast/osteocyte viability by limiting apoptotic cell death. We hypothesize that hsp27 is an estrogen- regulated protein that can promote osteoblast viability by increasing osteoblast resistance to apoptosis. The purpose of this study was to determine the effect of estrogen treatment and heat shock on TNF –induced apoptosis in the MC3T3-E1 cell line. Cells were treated with 0 – 100 nM 17 estradiol (or ICI 182780) for 0 – 24 hours before heat shock. After recovery, apoptosis was induced by treatment with 0 – 10 ng/ml TNF. Hsp levels were evaluated by Northern and Western analysis using hsp27, hsp47, hsp70c and hsp70i – specific reagents. Apoptosis was revealed by in situ labeling with Terminal Deoxyribonucleotide Transferase (TUNEL). A 5 – fold increase in hsp27 protein and mRNA was noted after 5 hours of treatment with 10–20 nM 17 estradiol prior to heat shock. Increased abundance of hsp47, hsp70c or hsp70i was not observed. TUNEL indicated that estrogen treatment also reduced (50%) MC3T3-E1 cell susceptibility to TNF – induced apoptosis. Treatment with hsp27-specific antisense oligonucleotides prevented hsp27 protein expression and abolished the protective effects of heat shock and estrogen treatment on TNF – induced apoptosis. Hsp27 is a determinant of osteoblast apoptosis, and estrogen treatment increases hsp27 levels in cultured osteoblastic cells. Hsp27 contributes to the control of osteoblast apoptosis and may be manipulated by estrogenic or alternative pathways for the improvement of bone mass.

참고문헌 (34)

  1. "Advances in apoptosis research.", 94, pp.12736~12737 (1997)
  2. "Heat shock proteins delivered with a virus vector can protect cardiac cells against apoptosis as well as against thermal or hypoxic stress.", 31, pp.135~146 (1999)
  3. "Role of the human heat shock protein hsp70 in protection against stress-induced apoptosis.", 17, pp.317~327 (1997)
  4. "Thermal preconditioning protects rat cardiac muscle cells from doxorubicin-induced apoptosis.", 64, pp.755~761 (1999)
  5. "Pyrrolidine dithiocarbamate activates the heat shock response and thereby induces apoptosis in primed endothelial cells.", 10, pp.1~6 (1998)
  6. "Delivery of a constitutively active form of the heat shock factor using a virus vector protects neuronal cells from thermal or ischaemic stress but not from apoptosis.", 10, pp.3343~3350 (1998)
  7. "Osteoblast programmed cell death apoptosis modulation by growth factors and cytokines", 13(5), pp.793~802 (1998)
  8. "The role of estrogen in the control of rat osteocyte apoptosis.", 13, pp.1243~1250 (1998)
  9. "Further observations on programmed cell death in the epiphyseal growth plate comparison of normal and dyschondroplastic epiphyses.", 12, pp.1647~1656 (1997)
  10. "Identification of apoptotic changes in osteocytes in normal and pathological human bone.", 20, pp.273~282 (1997)
  11. "Maruyama I Induction of apoptosis in murine clonal osteoblasts expressed by human T-cell leukemia virus type I tax by NF-kappa B and TNF-alpha.", 11, pp.200~210 (1996)
  12. "The death of osteocytes via apoptosis accompanies estrogen withdrawal in human bone.", 82, pp.3128~3135 (1997)
  13. "Uoshima K. Differential estrogenic regulation of small M heat shock protein expression in osteoblasts. J. Biol. Chem. 1994", pp.7869~7873
  14. "Estrogenic control of hsp 27 expression in calvarial organ culture.", 74, pp.51~ (1995)
  15. "Stein GS. Expression of heat shock genes during differentiation of mammalian osteoblasts and promyelocytic leukemia cells. J. Cell Biochem. 1992", pp.277~287
  16. "Synthesis of colony-stimulating factor and differentiation-inducing factor by osteoblastic cells, clone MC3T3-E1", 134(1), pp.400~406 (1996)
  17. "Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets", pp.4350~4354
  18. "In vivo evaluation of hsp 27 as an inhibitor of actin polymerization hsp 27 limits actin stress fiber and focal adhesion formation after heat shock.", 177, pp.575~584 (1998)
  19. "Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 1987", pp.156~159
  20. "Tumor necrosis factor-alpha confers resistance to hypoxic injury in the adult mammalian cardiac myocyte.", 97, pp.1392~1400 (1998)
  21. "Estrogen and NGF synergistically protect terminally differentiated, ERalpha- transfected PC12 cells from apoptosis", 56, pp.471~481 (1999)
  22. "Comparative study on the induction of cytostasis and apoptosis by ICI 182,780 and tamoxifen in an estrogen receptor-negative ovarian cancer cell line", 76, pp.47~54 (1998)
  23. "The estrogen receptor's involvement in osteoblasts' adaptive response to mechanical strain.", 13, pp.1275~1282 (1998)
  24. "Structural determinants affecting heat shock inducible transcription of the rat hsp27 gene.", 76, pp.49~ (1997)
  25. "Role of estrogen receptor", SP1 complexes in estrogen-induced heat shock protein 27 gene expression., 1996, pp.10, 1371~1378
  26. "Novel nuclear matrix protein HET binds to and influences activity of the HSP27 promoter in human breast cancer cells.", 67, pp.275~286 (1997)
  27. "Tissue-specific posttranslational modification of the small heat shock protein HSP27 in Drosophila.", 223, pp.1~8 (1996)
  28. "Estrogen decreases TNF gene expression by blocking JNK activity and the resulting production of c-Jun and JunD", 104, pp.503~513 (1996)
  29. "Effect of estrogen withdrawal on nitric oxide synthase expression and apoptosis in the rat vagina", 51, pp.650~656 (1998)
  30. "Effects of estrogen replacement therapy on plasma levels of nitric oxide in postmenopausal women", 180, pp.334~339 (1999)
  31. "Nitric oxide protects PC12 cells from serum deprivation-induced apoptosis by cGMP-dependent inhibition of caspase signaling", 19, pp.6740~6747 (1999)
  32. "Involvement of nitric oxide and biopterin in proinflammatory cytokine-induced apoptotic cell death in mouse osteoblastic cell line MC3T3-E1", 58, pp.649~655 (1999)
  33. "Estrogen promotes apoptosis of murine osteoclasts mediated by TGF-beta", 2, pp.1132~1136 (1996)
  34. "Increased bone formation by prevention of osteoblast apoptosis with parathyroidhormone", 104, pp.439~446 (1999)

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