식물생장조절제를 이용한 인삼 개갑률 향상 및 이후 발아와 식물체 형성에 미치는 영향
Improvement of Seed Dehiscence using Plant Growth Regulators and Its Effect on Subsequent Germination and Growth of Panax ginseng C. A. Meyer
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Plant growth regulators are frequently used to improve the dehiscence rate of seeds in Panax ginseng C. A. Meyer. However, research on the appropriate type and concentration of treatment is lacking. In addition, there is no information on the effects of plant growth regulator treatments on germination and plant formation before dehiscence. Methods and Results: Indehiscent seeds were soaked in gibberellic acid (GA ) and kinetin at con- centrations of 10 ㎎/ℓ – 300 ㎎/ℓ for 24 hours. The highest dehiscence rate was observed with the 10 ㎎/ℓ GA3 treatment. The dehiscence rate was significantly lower than that of the control, and the seed contamination rate significantly increased in the treatments containing 100 ㎎/ℓ or more GA3. GA3 treatment also affected the types of dehiscent seeds and embryo development. However, no differences were observed in the dehiscence rate or embryo development between the kinetin- treated and control groups. GA3 treatment before warm stratification affected seed germination but did not significantly affect plant development. Conclusions: In this study, we determined the type and concentration of plant growth regulators suitable for improving dehiscence and forming stable P. ginseng C. A. Meyer plants were pre- sented. These results are expected to contribute to stable ginseng seed production.
Abstract
Background: Plant growth regulators are frequently used to improve the dehiscence rate of seeds in Panax ginseng C. A. Meyer. However, research on the appropriate type and concentration of treatment is lacking. In addition, there is no information on the effects of plant growth regulator treatments on germination and plant formation before dehiscence. Methods and Results: Indehiscent seeds were soaked in gibberellic acid (GA ) and kinetin at con- centrations of 10 ㎎/ℓ – 300 ㎎/ℓ for 24 hours. The highest dehiscence rate was observed with the 10 ㎎/ℓ GA3 treatment. The dehiscence rate was significantly lower than that of the control, and the seed contamination rate significantly increased in the treatments containing 100 ㎎/ℓ or more GA3. GA3 treatment also affected the types of dehiscent seeds and embryo development. However, no differences were observed in the dehiscence rate or embryo development between the kinetin- treated and control groups. GA3 treatment before warm stratification affected seed germination but did not significantly affect plant development. Conclusions: In this study, we determined the type and concentration of plant growth regulators suitable for improving dehiscence and forming stable P. ginseng C. A. Meyer plants were pre- sented. These results are expected to contribute to stable ginseng seed production.
참고문헌 (30)
- Chen L, "Melatonin promotes seed germination under salt stress by regulating ABA and GA3 in cotton(Gossypium hirsutum L. )", Plant Physiology and Biochemistry, 162, pp.506~516 (2021)
- Chen S, "Roles of gibberellins and abscisic acid in dormancy and germination of red bayberry(Myrica rubra)seeds", Tree Physiology, 28, pp.1431~1439 (2008)
- Ge N, "Exogenous gibberellic acid shortening after-ripening process and promoting seed germination in a medicinal plant Panax notoginseng", BMC Plant Biology, 23, pp.67~ (2023)
- Groot SPC, "Gibberellins regulate seed germination in tomato by endosperm weakening : A study with gibberellin-deficient mutants", Planta, 171, pp.525~531 (1987)
- Han JW, "Seed morphology and germination characteristics of Rehmannia glutinosa", Korean Journal of Medicinal Crop Science, 30, pp.187~194 (2022)
- Hong CP, "Gibberellin signaling promotes the secondary growth of storage roots in Panax ginseng", International Journal of Molecular Sciences, 22, pp.8694~ (2021)
- Hovius MHY, "Effects of date and growth regulators on the culture of immature zygotic embryos of north american ginseng", Journal of Ginseng Research, 31, pp.14~22 (2007)
- Hu Y, "Gibberellins play an essential role in late embryogenesis of Arabidopsis", Nature Plants, 4, pp.289~298 (2018)
- Kabar K, "Comparative effects of kinetin, benzyladenine, and gibberellic acid on abscisic acid inhibited seed germination and seedling growth of red pine and arbor vitae", Turkish Journal of Botany, 22, pp.1~6 (1998)
- Karssen CM, "Key role for endogenous in the control of seed germination", Annals of Botany, 63, pp.71~80 (1989)
- Kim DH, "Flowering and fruits formation characteristics in major varieties of Panax ginseng", Korean Journal of Medicinal Crop Science, 24, pp.207~213 (2016)
- Kim MJ, "Enhancement of seed dehiscence by seed treatment with Talaromyces flavus GG01 and GG04 in ginseng(Panax ginseng)", Plant Pathology Journal, 33, pp.1~8 (2017)
- Kim YC, "Optimal harvesting time of ginseng seeds and effect of gibberellic acid(GA3)treatment for improving stratification rate of ginseng(Panax ginseng C. A. Meyer)seeds", Korean Journal of Medicinal Crop Science, 22, pp.423~428 (2014)
- Kim YH, "Regulation of endogenous and abscisic acid levels during different seed collection periods in Panax ginseng", Horticulture, Environment, and Biotechnology, 55, pp.166~174 (2014)
- Kwon WS, "Change of cytokinins and gibberellin contents during low temperature storage of dehisced ginseng seeds", Journal of the Korean Society for Horticultural Science, 38, pp.111~115 (1997)
- Lee JW, "Improvement of seed dehiscence and germination in ginseng by stratification, gibberellin, and", or kinetin treatments, 2018, pp.59, 293~301
- Lee JW, "Efficient micropropagation of genetically stable Panax ginseng Meyer by somatic embryogenesis", Agronomy, 13, pp.1139~ (2023)
- Lee JW, "Efficient somatic embryogenesis, regeneration and acclimatization of Panax ginseng Meyer : True-to-type conformity of plantlets as confirmed by ISSR analysis", Plants, 12, pp.1270~ (2023)
- Lee JW, "Effects of gibberellic acid and alternating temperature on breaking seed dormancy of Panax ginseng C. A. Meyer", Korean Journal of Medicinal Crop Science, 24, pp.284~293 (2016)
- Lee JW, "In vitro micro-propagation of commercial ginseng cultivars(Panax ginseng Meyer)via somatic embryogenesis compared to traditional seed production", Horticulturae, 9, pp.435~ (2023)
- Lim SH, "Influence of GA3 soak and seed dressing with Toros(tolclofos methyl)wp. on the dehiscence of Eleutherococcus senticosus Maxim seeds", Korean Journal of Medicinal Crop Science, 16, pp.106~111 (2008)
- Min JE, "The dehiscence process in Panax ginseng seeds and the stigmasterol biosynthesis pathway in terms of metabolomics", Journal of Ginseng Research, 46, pp.225~234 (2022)
- Park H, "Effect of gibberellin and kinetin on bud dormancy breaking and growth of Korean ginseng root(Panax ginseng C. A. meyer. )", Journal of Ginseng Research, 3, pp.105~112 (1979)
- Prasad VN, "Alleviation by gibberellic acid and kinetin of the inhibition of seed germination in Maize(Zea mays L. )under submerged conditions", Annals of Botany, 52, pp.649~652 (1983)
- Ross JD, "Studies in seed dormancy. VI. The effects of growth retardants on the gibberellin content and germination of chilled seeds of Corylus avellana L", Planta, 100, pp.303~308 (1971)
- Skoog F, "Cytokinins", Annual Review of Plant Biology, 21, pp.359~384 (1970)
- Soppe WJJ, "Seed dormancy back on track; its definition and regulation by DOG1", The New Phytologist, 228, pp.816~819 (2020)
- Suh SJ, "Effect of seed moisture content on seed storage of dehisced ginseng seeds", Korean Journal of Plant Resources, 32, pp.457~462 (2022)
- Tuan PA, "A shift in abscisic acid", gibberellin balance underlies retention of dormancy induced by seed development temperature, 2021, pp.44, 2230~2244
- Yang L, "The role of light in regulating seed dormancy and germination", Journal of Integrative Plant Biology, 62, pp.1310~1326 (2020)