Line Probe Assay 를 이용한 Rifampicin 내성 결핵균의 신속한 검출

Abstract

Control of tuberculosis are compromised by the increased frequency of multidrug - resistant strains of Mycobacterium tuberculosis(M. tuberculosis). Rifampicin is a key compo nent of therapeutic regimens. Resistance to rifampicin results from the substitution of a limited number of highly conserved amino acids encoded by rpo β the gene for the βsubunit of the RNA polymerase. In present study, nested - PCR coupled line probe assay methods were used for rapid detection of rifampicin resistant strain in PCR positive samples with M. tuberculosis. In a total of 10 nested - PCR M. tuberculosis positive samples, including 8 sputums, 1 pleural fluid and 1 urine, three rifampicin resistant strains (30%) were detected. Among these, one mutation was found in Sl locus of rpo ß gene, and other two mutations were observed in coinfection state of S2/R5 loci and S4/R4a loci, respectively. This study shows that nested - PCR coupled line probe assay is a relatively simple, fast and practical tool for the detection rifampicin resistant M. tuberculosis strains in the routine clinical laboratory work.