사과 왜성대목 M.9 및 M.26의 고온, ribavirin, 생장점 배양을 통한 바이러스 제거
Efficient virus elimination for apple dwarfing rootstock M.9 and M.26 via thermotherapy, ribavirin and apical meristem culture
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Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy (37°C, 6 weeks) and chemotherapy(RibavirinⓇ). The detection of apple viruses was accomplished by Enzyme-linked Immuno- Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR method was 10 ~ 30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30 ~ 40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.
Abstract
Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy (37°C, 6 weeks) and chemotherapy(RibavirinⓇ). The detection of apple viruses was accomplished by Enzyme-linked Immuno- Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR method was 10 ~ 30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30 ~ 40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.
참고문헌 (34)
- Campbell AI, "Apple virus inactivation by heat therapy and tip propagation", Nature, 195, pp.520~ (1962)
- Chellappan P, "Effect of temperature on geminivirus-induced RNA silencing in plants", Plant Physiology, 138, pp.1828~1841 (2005)
- 조강희, "항바이러스제 처리와 경정배양에 의한 배(Pyrus pyrifolia L.) ‘만수’의 Apple stem grooving virus 무병화", Journal of Plant Biotechnology, 43(3), pp.391~396 (2016), 한국식물생명공학회
- Cooper VC, "Thermal inactivation of Cherry leaf roll virus in tissue cultures of Nicotiana rustica raised from seeds and meristem tips", Annals of Applied Biology, 88, pp.273~278 (1978)
- Dixon-Holland DE, "Competitive direct enzyme-linked immunosorbent screening assay for the detection of sulfamethazine contamination of animal feeds", Journal Association of Analysis Chemistry International, 74, pp.784~789 (1991)
- El-Dougdoug KA, "Elimination of Hop stunt viroid (HSVd)from infected peach and pear plants", Australian Journal of Basic and Applied Science, 4, pp.54~60 (2010)
- Faccioli VC, "In plant virus disease control", pp.346~380 (1998), American Phytopathological Society
- Feng C, "Production of pathogen-free horticultural crops by cryotherapy of in vitro-Grown shoot tips", Methods in Molecular Biology, 994, pp.463~482 (2013)
- Gambino G, "A rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants", Phytochemical Analysis, 19, pp.520~525 (2008)
- Hansen A, "Elimination of apple chlorotic leaf spot virus from apple shoot cultures by ribavirin", Plant Disease, 69, pp.134~135 (1985)
- Hansen AJ, "Antiviral chemicals for plant disease control", Critical Reviews in Plant Science, 8, pp.45~88 (1989)
- Hollings M, "Disease control through virus-free stock", Annual Review of Phytopathology, 3, pp.367~396 (1965)
- Hu G, "Virus elimination from in vitro apple by thermotherapy combined with chemotherapy", Plant Cell Tissue Culture, 121, pp.435~443 (2015)
- Joung YH, "An Effective Detection of Potato Virus Y Using RT-PCR Technique", Korean Journal Plant Pathology, 13, pp.219~224 (1997)
- 김정수, "2010년 우리나라 주요 작물 바이러스병 발생 상황", 식물병 연구, 17(3), pp.334~341 (2011), 한국식물병리학회
- 김미영, "사과 품종 홍로의 휴면아 분열조직 배양을 통해 형성된 캘러스에서의 바이러스 제거효율", Journal of Plant Biotechnology, 44(4), pp.379~387 (2017), 한국식물생명공학회
- Kinard, G, "Detection of apple chlorotic leaf spot and apple stem grooving viruses using RT-PCR", Plant Disease, 80, pp.616~621 (1996)
- Knapp E, "Distribution of Apple chlorotic leaf spot virus in apple shoots cultivated in vitro", Acta Horticuture, 386, pp.187~194 (1995)
- "KOSIS(Korea statistical Information Service)", ~ (2018)
- 이건섭, "열처리와 경정배양을 이용한 바이러스 무병 사과 생산 시스템", 식물병 연구, 19(4), pp.288~293 (2013), 한국식물병리학회
- 이성희, "국내 유통 주요 사과나무 묘목의 바이러스 감염 실태", 식물병 연구, 23(4), pp.355~362 (2017), 한국식물병리학회
- Liu P, "Detection and molecular variability of Apple stem grooving virus in Shaanxi, China", Journal Phytopathology, 161, pp.445~449 (2013)
- Menzel W, "Detection of four apple viruses by multiplex RT-PCR assays with complification of plant mRNA as internal control", Journal Virological Methods, 99, pp.81~92 (2002)
- Murashige, "A revised medium for rapid growth and bioassays with tobacco tissue culture", Physiology plant, 15, pp.473~497 (1962)
- Paduch-Cichal E, "A low temperature therapy and meristem-tip culture for eliminating four viroids from infected plants", Journal Phytopathology, 118, pp.341~346 (1987)
- Paprstein F, "Results of in vitro thermotherapy of apple cultivars", Plant Cell Tissue Culture, 94, pp.347~352 (2008)
- Plopa C, "Elimination of Apple mosaic virus by tissue culture of some infected apple cultivars", Acta Horticulture, 981, pp.517~522 (2013)
- Wina Dian Savitri, "Elimination of Chrysanthemum stunt viroid (CSVd) from Meristem Tip Culture Combined with Prolonged Cold Treatment", Horticulture, Environment, and Biotechnology, 54(2), pp.177~182 (2013), 한국원예학회[DOI]
- Smith PR, "Aetiology of the rosette and decline diseases of peach and interactions between Prunus necrotic ringspot, Prune dwarf, and dark green sunken mottle viruses", Australian Journal of Agriculture Research, 23, pp.1027~1034 (1972)
- Tan R, "Enhanced efficiency of virus eradication following thermotherapy of shoot-tip cultures of pear", Plant Cell Tissue and Organ Culture, 101, pp.229~235 (2010)
- Verma N, "In vitro production of Prunus necrotic ringspot virus-free begonias through chemo-and thermotherapy", Science Horticulture, 103, pp.239~247 (2005)
- Wang L, "Effect of thermotherapy on elimination of Apple stem grooving virus and Apple chlorotic leaf spot virus for in vitro-cultured pear shoot tips", Horticulture Science, 41, pp.729~732 (2006)
- Wang QC, "Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips", Molecular Plant Pathology, 9, pp.237~250 (2008)
- Wood GA, "Virus and Virus-Like Diseases of Pome Fruits And Stone Fruits in New Zealand. No. 226", Bulletin, Department of Scientific and Industrial Research, 87~ (1979)