오비트랩 방식의 액체크로마토그래피 고분해능 정밀질량 분석방법을 적용한 수질 트리아진계 화합물의 미량분석방법
Analysis of Triazines in Water using Orbitrap High Resolution and Accurate Mass Spectrometer
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초록
Full scanning technology by high resolution and accurate mass spectrometer (HRAM) is one of most advanced methodology for identification and quantification of organic compounds. In this study, column switching technique based online preconcentration method was combined with high resolution full scan mass spectrometer to measure trizazine pesticides in water. After parameter optimization of online preconcentration,1 mL volume of filtered sample was used. For instrumental analysis, electrospray ionization (ESI) and orbitrap mass spectrometer with 100~600 amu scan range at 50,000 resolving power was tested. By this approach,available mass accuracy below 2.7 ppm (parts per million of monoisotopic mass), calibration linearities above 0.997, method detection limit (MDL) for 0.2~1.8 pg/mL, recoveries for 102~117% and repeatabilities below 4.3% were obtained. Method performance of online preconcentration with HRAM method is comparable to the state-of-the-art solid-phase extraction (SPE) with triple quadrupole mass spectrometer (MS/MS).
Abstract
Full scanning technology by high resolution and accurate mass spectrometer (HRAM) is one of most advanced methodology for identification and quantification of organic compounds. In this study, column switching technique based online preconcentration method was combined with high resolution full scan mass spectrometer to measure trizazine pesticides in water. After parameter optimization of online preconcentration,1 mL volume of filtered sample was used. For instrumental analysis, electrospray ionization (ESI) and orbitrap mass spectrometer with 100~600 amu scan range at 50,000 resolving power was tested. By this approach,available mass accuracy below 2.7 ppm (parts per million of monoisotopic mass), calibration linearities above 0.997, method detection limit (MDL) for 0.2~1.8 pg/mL, recoveries for 102~117% and repeatabilities below 4.3% were obtained. Method performance of online preconcentration with HRAM method is comparable to the state-of-the-art solid-phase extraction (SPE) with triple quadrupole mass spectrometer (MS/MS).
참고문헌 (23)
- M. Natangelo, , J Chromatogr A, 859, pp.193~201 (1999)
- S. Barrek, , Talanta, 79(3), pp.712~722 (2009)
- U.S. EPA, "U.S. EPA Method 527. Rei. 1.0, Determination of selected pesticides and flame retardants in drinking water by solid phase extraction and capillary column gas chromatography", mass spectrometry (GC, pp.2005, ~
- U.S. EPA, "U.S. EPA Method 536, Determination of triazine pesticides and their degradates in drinking water by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS", MS), 2007, CINCINNATI, OHIO, 2007
- U.S. EPA, "U.S. EPA Method 539, Determination of hormones in drinking water by solid phase extraction (SPE) and liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS", MS), 2010, CINCINNATI, OHIO, 2010
- A.C. Hogenboom, , Rapid Comm. Mass Spec., 13(2), pp.125~133 (2009)
- I. Bobeldijk, , J Chromatogr A, 929(1-2), pp.63~74 (2001)
- J. Hau, , Rapid Comm. Mass Spec., 15(19), pp.1840~1848 (2001)
- T. Kosjek, , J Chromatogr A, 1215(1-2), pp.57~63 (2008)
- M. Krauss, , Analytical and Bioanalytical Chemistry, 397(3), pp.943~951 (2010)
- E. van der Heeft, , JASMS, 20, pp.451~463 (2009)
- Y.Y. Zou, , 60th ASMS (2012)
- S. Reddy, , 60th ASMS (2012)
- R. Pingancos, , 60th ASMS (2012)
- T. Kosjek, , 60th ASMS (2012)
- S. Thach, , 60th ASMS (2012)
- EU SANCO, "EU SANCO Method 12495, Validation and quality control procedures for pesticide residues analysis in food and feed, Implemented document", 18~ (2012)
- G.A. Araceli, , J Chromatogr A., 1216(48), pp.8518~8527 (2009)
- P.A. Segura, , Chemosphere, 84(8), pp.1085~1094 (2011)
- P.A. Segura, , Chromatographia, 70(2), pp.239~245 (2009)
- J. Beck, , 60th ASMS (2012)
- K.P. Bateman, , J Am Soc Mass Spectrom, 20, pp.1441~1450 (2009)
- 장제헌, "온라인 시료주입과 UPLC-Orbitrap 질량분석법을 이용한 수질 조류독소의 고속분석방법 개발 및 환경시료적용", 한국물환경학회지, 28(6), pp.843~850 (2012), 한국물환경학회