DART-Orbitrap 질량분석방법 중 내부표준법에 의한수질의 atrazine, simazine의 정량 분석
Quantitation by DART-Orbitrap Mass Spectrometer: Application of Atrazine and Simazine in Water by Internal Standards
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Ambient sample ionization by direct analysis in real time (DART), with high resolution and accurate mass spectrometry (HR/AM), was applied to quantitation of atrazine and simazine in water. This system needs 0.5min for sample run in mass spectrometer without sample preparation. As an internal standard, compounds which having similar physico-chemical properties such as deuterium isotope atrazine-d5 and simazine-d10were compared. Optimal sample loading conditions such as loading volume and drying time were validated. For example, three to five μL loading volume on the disposable mesh card, and 20 min of sample drying conditions showed high sensitivities. In 1 to 50 μg/L calibration ranges, Available calibration linearity coefficients were obtained for 0.9835~0.9997. QA/QC samples using 1, 2.5 and 5 μg/L showed comparable averaged recovery rates between 94~123%. In the 1 μg/L and 2.5 μg/L samples, practical quantitation limits (PQL) were 1.2~2.7 μg/L. During experiments, bench top Orbitrap mass spectrometry showed under 3 ppm mass accuracy at the external calibration. Results of this study indicated that physico-chemical property such as vapour pressure is important factor in quantitative method of choice for ambient ionization.
Abstract
Ambient sample ionization by direct analysis in real time (DART), with high resolution and accurate mass spectrometry (HR/AM), was applied to quantitation of atrazine and simazine in water. This system needs 0.5min for sample run in mass spectrometer without sample preparation. As an internal standard, compounds which having similar physico-chemical properties such as deuterium isotope atrazine-d5 and simazine-d10were compared. Optimal sample loading conditions such as loading volume and drying time were validated. For example, three to five μL loading volume on the disposable mesh card, and 20 min of sample drying conditions showed high sensitivities. In 1 to 50 μg/L calibration ranges, Available calibration linearity coefficients were obtained for 0.9835~0.9997. QA/QC samples using 1, 2.5 and 5 μg/L showed comparable averaged recovery rates between 94~123%. In the 1 μg/L and 2.5 μg/L samples, practical quantitation limits (PQL) were 1.2~2.7 μg/L. During experiments, bench top Orbitrap mass spectrometry showed under 3 ppm mass accuracy at the external calibration. Results of this study indicated that physico-chemical property such as vapour pressure is important factor in quantitative method of choice for ambient ionization.
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