The effects of Cudrania tricuspidata extract on bone metabolism in ovariectomized rats
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The effects of Cudrania tricuspidata (CT) extract on markers of osteoporosis were examined in ovariectomized rats. We classified 26 rats into five groups and provided a pellet chow diet and tap water throughout the 27-wk experimental period. During the last 15 wk, we added oral injections to each group as follows: sham-operated (SHAM, n=4) and ovariectomized-control (OVX, n=5) with distilled water, alendronate with 10 mg/kg/d of alendronate sodium (ALEN, n=5), CT (CT100, n=6) with 100 mg/kg/d of CT, and CT (CT300, n=6) with 300 mg/kg/d of CT. After the experimental period, blood, urine, and micro-CT images were assessed. The CT100 and OVX groups did not show any significant differences in urinary n-terminal telopeptide (NTx) (p<0.05 ), but with increases in CT concentration, the NTx level was slightly reduced. Serum osteocalcin was significantly higher in the CT groups than in all other groups (p<0.05 ). Notably, the serum calcium levels of all groups were within the normal range, but urinary calcium levels in the CT groups were significantly lower than the OVX group (p<0.05 ). In addition, the CT groups exhibited higher trabecular BMD than the OVX groups while showing similar BMD to the ALEN group (p<0.05 ). The Tb.Th of the ALEN group was lower than all other groups. Based on the overall analysis of results, CT prevented bone loss by inhibiting bone resorption and enhancing bone formation. Although alendronate showed a similar effect in preventing bone loss, it did so by solely inhibiting bone resorption, and its long-term use reportedly causes paradoxical effects such as hip fractures. Thus, for osteoporosis induced by ovariectomy, we conclude that CT extract is an effective natural treatment without severe side effects.
Abstract
The effects of Cudrania tricuspidata (CT) extract on markers of osteoporosis were examined in ovariectomized rats. We classified 26 rats into five groups and provided a pellet chow diet and tap water throughout the 27-wk experimental period. During the last 15 wk, we added oral injections to each group as follows: sham-operated (SHAM, n=4) and ovariectomized-control (OVX, n=5) with distilled water, alendronate with 10 mg/kg/d of alendronate sodium (ALEN, n=5), CT (CT100, n=6) with 100 mg/kg/d of CT, and CT (CT300, n=6) with 300 mg/kg/d of CT. After the experimental period, blood, urine, and micro-CT images were assessed. The CT100 and OVX groups did not show any significant differences in urinary n-terminal telopeptide (NTx) (p<0.05 ), but with increases in CT concentration, the NTx level was slightly reduced. Serum osteocalcin was significantly higher in the CT groups than in all other groups (p<0.05 ). Notably, the serum calcium levels of all groups were within the normal range, but urinary calcium levels in the CT groups were significantly lower than the OVX group (p<0.05 ). In addition, the CT groups exhibited higher trabecular BMD than the OVX groups while showing similar BMD to the ALEN group (p<0.05 ). The Tb.Th of the ALEN group was lower than all other groups. Based on the overall analysis of results, CT prevented bone loss by inhibiting bone resorption and enhancing bone formation. Although alendronate showed a similar effect in preventing bone loss, it did so by solely inhibiting bone resorption, and its long-term use reportedly causes paradoxical effects such as hip fractures. Thus, for osteoporosis induced by ovariectomy, we conclude that CT extract is an effective natural treatment without severe side effects.
참고문헌 (32)
- Arslan A, "Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats", Libyan J Med, 6, pp.5965~ (2011)
- Ataoğlu MB, "A comparison of the measurements with biochemical markers of bone turnover and bone mineral density in the assessment of the efficiency of osteoporosis treatment", Eklem Hast Cerrahisi, 24, pp.82~86 (2013)
- Baeksgaard L, "Calcium and vitamin D supplementation increases spinal BMD in healthy, postmenopausal women", Osteoporosis Int, 8, pp.255~260 (1998)
- Bharadwaj S, "Milk ribonuclease-enriched lactoferrin induces positive effects on bone turnover markers in postmenopausal women", Osteoporosis Int, 20, pp.1603~1611 (2009)
- Chen WF, "Total flavonoid fraction of the Herba epimedii extract suppresses urinary calcium excretion and improves bone properties in ovariectomised mice", Brit JNutr, 105, pp.180~189 (2011)
- 최미자, "비타민 K2 첨가 식이가 난소절제쥐의 골밀도에 미치는 영향", Journal of Nutrition and Health, 44(3), pp.189~195 (2011), 한국영양학회
- 김소헌, "Association between Nutrient Intake and Obesity in Type 2 Diabetic Patients from the Korean National Diabetes Program: A Cross-Sectional Study", Journal of Korean Medical Science, 27(10), pp.1188~1195 (2012), 대한의학회
- Dandinoğlu T, "Can long-term bisphosphonate use causes low-energy fractures? A case report", Osteoporosis Int, 25, pp.773~776 (2013)
- Epstein S, "Oral ibandronate in the management of postmenopausal osteoporosis: review of upper gastrointestinal safety", Maturitas, 54, pp.1~10 (2006)
- Goffinet M, "Zoledronic acid treatment impairs protein geranyl-geranylation for biological effects in prostatic cells", BMC Cancer, 6, pp.60~ (2006)
- Green J, "Oral bisphosphonates and risk of cancer of oesophagus, stomach, and colorectum: case-control analysis within a UK primary care cohort", BMJ- Brit Med J, 341, pp.c4444~ (2010)
- Halasy-Nagy JM, "Inhibition of bone resorption by alendronate and risedronate does not require osteoclast apoptosis", Bone, 29, pp.553~559 (2001)
- Imai K, "Alendronate sodium hydrate (oral jelly) for the treatment of osteoporosis: review of a novel, easy to swallow formulation", Clin Interv Aging, 8, pp.681~688 (2013)
- Kalu DN, "The ovariectomized rat model of postmenopausal bone loss", Bone and mineral, 15, pp.175~191 (1991)
- Kanis JA, "Evaluation of the risk of hip fracture", Bone, 18, pp.S127~S132 (1996)
- Karsenty G, "Regulation of male fertility by the bonederived hormone osteocalcin", Mol Cell Endocrinol, 382, pp.521~526 (2013)
- Kennedy RL, "The vitamin K-dependent Gla proteins and risk of type 2 diabetes", Diabetologia, 56, pp.2100~2101 (2013)
- 김미숙, "꾸지뽕나무(Cudrania tricuspidata) 잎의 추출물이 대장암세포주에 미치는 영향", 대한구강악안면병리학회지, 33(1), pp.19~26 (2009), 대한구강악안면병리학회
- 권해용, "Effects of silk fibroin hydrolysate on bone metabolism in ovariectomized rats", International Journal of Industrial Entomology, 30(1), pp.17~25 (2015), 한국잠사학회
- Lauritzen JB, "Hip fractures: incidence, risk factors, energy absorption, and prevention", Bone, 18, pp.S65~S75 (1996)
- Lee NK, "Endocrine regulation of energy metabolism by the skeleton", Cell, 130, pp.456~469 (2007)
- Li N, "Naringin promotes osteoblast differentiation and effectively reverses ovariectomyassociated osteoporosis", J Orthop Sci, 18, pp.478~485 (2013)
- Lim YW, "Etiology of Osteoporosis and Prevention of Osteoporotic Fractures (in Korean)", J Kor Orthopedic Assoc, 21, pp.6~16 (2009)
- Melton LJ, "Heterogeneity of age-related fractures: implications for epidemiology", Bone and mineral, 2, pp.321~ (1987)
- Mizuguchi M, "Fourier-transform infrared spectroscopic study of Ca2+-binding to osteocalcin", Calcified Tissue Int, 69, pp.337~342 (2001)
- Mundy GR, "Inflammatory mediators and the destruction of bone", J Periodontal Res, 26, pp.213~217 (1991)
- Nordin BEC, "Calcium and osteoporosis", Nutrition, 13, pp.664~686 (1997)
- Odvina CV, "Severely suppressed bone turnover: a potential complication of alendronate therapy", J Clin Endocr Metab, 90, pp.1294~1301 (2005)
- Puchacz E, "Chromosomal localization of the human osteocalcin gene", Endocrinol, 124, pp.2648~2650 (1989)
- Rodan GA, "Mechanisms of action of bisphosphonates", Annu Rev Pharmacol Toxicol, 38, pp.375~388 (1998)
- Tranquilli AL, "Calcium, phosphorus and magnesium intakes correlate with bone mineral content in postmenopausal women", Gynecol Endocrinol, 8, pp.55~58 (1994)
- Vlasiadis KZ, "Relationship between BMD, dental panoramic radiographic findings and biochemical markers of bone turnover in diagnosis of osteoporosis", Maturitas, 59, pp.226~233 (2008)