Taurine effects on cell proliferation and gene expression of apoptosis and oxidative stress in broiler satellite cells under heat stress
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This study aimed to investigate the effects of taurine on broiler embryonic muscle-derived satellite cells under heat stress. Satellite cells were extracted from the leg muscle of 18-day-old broiler embryos and cultured under two different temperature (37℃ and 42℃), with or without taurine addition. The experiment comprised four treatment groups: 37℃ without taurine, 37℃ with taurine, 42℃ without taurine, and 42℃ with taurine. Cell survival rates and the expression of genes related to heat shock proteins (HSPs), cell cycle, oxidative stress, and MAPK/ERK-Nrf2 were analyzed, considering the effects of taurine and culture temperature and their interaction. The results showed higher cell survival rates at 42℃, with increased expression of satellite cell markers pax7 and MyoD in all treatment groups. HSP90 and HSP40 gene expressions were significantly upregulated at 42℃, and taurine treatment also led to a significant increase. However, HSP70 expression showed the opposite pattern. In cell cycle-related genes, expect for bcl2, significant differences were observed due to taurine and culture temperature, with an interaction effect observed for p53. Regarding antioxidant-related gene expression, superoxide dismutase was significantly upregulated at 42℃ and also in the taurine treatment. extracellular signal-regulated kinase and nuclear factor erythroid 2-related factor 2 showed significant increases and decreases due to 42℃ and taurine, respectively, while c-Jun N-terminal kinase and p38 displayed opposing results. These diverse findings suggest that broiler satellite cells can undergo proliferation and growth at 42℃, and taurine treatment can enhance their resilience to heat stress, potentially maintaining cellular health even at 37℃.
Abstract
This study aimed to investigate the effects of taurine on broiler embryonic muscle-derived satellite cells under heat stress. Satellite cells were extracted from the leg muscle of 18-day-old broiler embryos and cultured under two different temperature (37℃ and 42℃), with or without taurine addition. The experiment comprised four treatment groups: 37℃ without taurine, 37℃ with taurine, 42℃ without taurine, and 42℃ with taurine. Cell survival rates and the expression of genes related to heat shock proteins (HSPs), cell cycle, oxidative stress, and MAPK/ERK-Nrf2 were analyzed, considering the effects of taurine and culture temperature and their interaction. The results showed higher cell survival rates at 42℃, with increased expression of satellite cell markers pax7 and MyoD in all treatment groups. HSP90 and HSP40 gene expressions were significantly upregulated at 42℃, and taurine treatment also led to a significant increase. However, HSP70 expression showed the opposite pattern. In cell cycle-related genes, expect for bcl2, significant differences were observed due to taurine and culture temperature, with an interaction effect observed for p53. Regarding antioxidant-related gene expression, superoxide dismutase was significantly upregulated at 42℃ and also in the taurine treatment. extracellular signal-regulated kinase and nuclear factor erythroid 2-related factor 2 showed significant increases and decreases due to 42℃ and taurine, respectively, while c-Jun N-terminal kinase and p38 displayed opposing results. These diverse findings suggest that broiler satellite cells can undergo proliferation and growth at 42℃, and taurine treatment can enhance their resilience to heat stress, potentially maintaining cellular health even at 37℃.